LOCUS       sequence-240660-        1860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS495
FEATURES             Location/Qualifiers
     source          1..1860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(227..261)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(332..991)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     906..925
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1173..1761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1662..1681
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc attgatatat atagcggtag tgtttgcgcg ttaccatcat cttctggatc
       61 tatctattgt tcttttcctc atcactttcc cctttttcgc tcttcttctt gtcttttatt
      121 tctttctttt ttttaattgt tccctcgatt ggctatctac caaagaatcc aaacttaata
      181 cacgtattta tttgtccaat taccagatct atgtgagacc agaccaataa aaaacgcccg
      241 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
      301 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt
      361 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
      421 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
      481 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
      541 cccagggatt ggctgaaacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
      601 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
      661 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
      721 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
      781 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
      841 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
      901 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
      961 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     1021 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     1081 ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1141 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1201 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1261 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1321 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1381 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1441 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1501 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     1561 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1621 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1681 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1741 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     1801 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
//
