LOCUS       sequence-240699-        7238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression for funtional studies of cell growth promotion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa 
            J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M
  TITLE     Identification, basic characterization and evolutionary analysis of 
            differentially spliced mRNA isoforms of human YAP1 gene.
  JOURNAL   Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. 
            Epub 2012 Aug 24.
  PUBMED    22939869
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gene.2012.08.025"; journalName: "Gene"; date: 
            "2012-11-10- 10"; volume: "509"; issue: "2"; pages: "215-22"
COMMENT     Sequence Label: pBABE (hygro) mEOS2 hYAP 1-1beta
FEATURES             Location/Qualifiers
     source          1..7238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             434..1111
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     primer_bind     complement(2494..2514)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2499..2828
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2679..2814
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2902..3927
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2902..3927
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     4444..4463
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4725..5194
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(5309..6100,6101..6169))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5932..5951
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6170..6274)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             6305..6774
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6839..7038
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
       61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc
      421 cggatccgcc accatgagtg cgattaagcc agacatgaag atcaaactcc gtatggaagg
      481 caacgtaaac gggcaccact ttgtgatcga cggagatggt acaggcaagc cttttgaggg
      541 aaaacagagt atggatcttg aagtcaaaga gggcggacct ctgccttttg cctttgatat
      601 cctgaccact gcattccatt acggcaacag ggtattcgcc aaatatccag acaacataca
      661 agactatttt aagcagtcgt ttcctaaggg gtattcgtgg gaacgaagct tgactttcga
      721 agacgggggc atttgcattg ccagaaacga cataacaatg gaaggggaca ctttctataa
      781 taaagttcga ttttatggta ccaactttcc cgccaatggt ccagttatgc agaagaagac
      841 gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt gatggagtgc tgacgggtga
      901 tattcatatg gctttgttgc ttgaaggaaa tgcccattac cgatgtgact tcagaactac
      961 ttacaaagct aaggagaagg gtgtcaagtt accaggctac cactttgtgg accactgcat
     1021 tgagatttta agccatgaca aagattacaa caaggttaag ctgtatgagc atgctgttgc
     1081 tcattctgga ttgcctgaca atgccagacg agaattcatg gatcccgggc agcagccgcc
     1141 gcctcaaccg gccccccagg gccaagggca gccgccttcg cagcccccgc aggggcaggg
     1201 cccgccgtcc ggacccgggc aaccggcacc cgcggcgacc caggcggcgc cgcaggcacc
     1261 ccccgccggg catcagatcg tgcacgtccg cggggactcg gagaccgacc tggaggcgct
     1321 cttcaacgcc gtcatgaacc ccaagacggc caacgtgccc cagaccgtgc ccatgaggct
     1381 ccggaagctg cccgactcct tcttcaagcc gccggagccc aaatcccact cccgacaggc
     1441 cagtactgat gcaggcactg caggagccct gactccacag catgttcgag ctcattcctc
     1501 tccagcttct ctgcagttgg gagctgtttc tcctgggaca ctgaccccca ctggagtagt
     1561 ctctggccca gcagctacac ccacagctca gcatcttcga cagtcttctt ttgagatacc
     1621 tgatgatgta cctctgccag caggttggga gatggcaaag acatcttctg gtcagagata
     1681 cttcttaaat cacatcgatc agacaacaac atggcaggac cccaggaagg ccatgctgtc
     1741 ccagatgaac gtcacagccc ccaccagtcc accagtgcag cagaatatga tgaactcggc
     1801 ttcagccatg aaccagagaa tcagtcagag tgctccagtg aaacagccac cacccctggc
     1861 tccccagagc ccacagggag gcgtcatggg tggcagcaac tccaaccagc agcaacagat
     1921 gcgactgcag caactgcaga tggagaagga gaggctgcgg ctgaaacagc aagaactgct
     1981 tcggcaggtg aggccacagg agttagccct gcgtagccag ttaccaacac tggagcagga
     2041 tggtgggact caaaatccag tgtcttctcc cgggatgtct caggaattga gaacaatgac
     2101 gaccaatagc tcagatcctt tccttaacag tggcacctat cactctcgag atgagagtac
     2161 agacagtgga ctaagcatga gcagctacag tgtccctcga accccagatg acttcctgaa
     2221 cagtgtggat gagatggata caggtgatac tatcaaccaa agcaccctgc cctcacagca
     2281 gaaccgtttc ccagactacc ttgaagccat tcctgggaca aatgtggacc ttggaacact
     2341 ggaaggagat ggaatgaaca tagaaggaga ggagctgatg ccaagtctgc aggaagcttt
     2401 gagttctgac atccttaatg acatggagtc tgttttggct gccaccaagc tagataaaga
     2461 aagctttctt acatggttat aggtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa
     2521 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     2581 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     2641 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     2701 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     2761 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     2821 tttgcaaaaa gcttgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcagatct
     2881 gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     2941 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     3001 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     3061 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     3121 attccggaag tgcttgacat tggggaattt agcgagagcc tgacctattg catctcccgc
     3181 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     3241 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     3301 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     3361 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     3421 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     3481 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     3541 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     3601 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     3661 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     3721 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     3781 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     3841 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     3901 agcactcgtc cgagggcaaa ggaatagagt agatgccgac cgggatctat cgatgcggcg
     3961 ggtgtggtgg ttacgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4021 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4081 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4141 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4201 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4261 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4321 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4381 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4441 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4501 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcatc
     4561 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
     4621 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact
     4681 gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa
     4741 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc
     4801 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     4861 aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt
     4921 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     4981 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     5041 cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat
     5101 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
     5161 gagtgattga ctacccgtca gcgggggtct ttcacatgca gcatgtatca aaattaattt
     5221 ggtttttttt cttaagtatt tacattaaat ggccatagtg cggccgcaaa tcaatctaaa
     5281 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     5341 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     5401 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     5461 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     5521 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5581 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5641 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5701 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5761 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5821 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5881 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5941 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6001 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6061 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6121 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6181 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     6241 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     6301 cagcctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     6361 ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
     6421 tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt
     6481 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
     6541 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
     6601 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
     6661 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
     6721 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg
     6781 ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggcaa
     6841 gctggccagc aacttatctg tgtctgtccg attgtctagt gtctatgact gattttatgc
     6901 gcctgcgtcg gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg
     6961 acgagttctg aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt
     7021 tttgtggccc gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg
     7081 ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg
     7141 tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc
     7201 tctgtctgac tgtgtttctg tatttgtctg aaaattag
//
