LOCUS       sequence-240718-        6734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression for funtional studies of cell growth promotion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa 
            J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M
  TITLE     Identification, basic characterization and evolutionary analysis of 
            differentially spliced mRNA isoforms of human YAP1 gene.
  JOURNAL   Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. 
            Epub 2012 Aug 24.
  PUBMED    22939869
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gene.2012.08.025"; journalName: "Gene"; date: 
            "2012-11-10- 10"; volume: "509"; issue: "2"; pages: "215-22"
COMMENT     Sequence Label: pBABE (hygro) hYAP 1-2delta
FEATURES             Location/Qualifiers
     source          1..6734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1573..1593)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1578..1907
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1758..1893
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1981..3006
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1981..3006
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     3523..3542
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             3804..4273
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(4388..5179,5180..5248))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5011..5030
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5249..5353)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5384..5853
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5918..6117
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             6318..6734
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6664..6686
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6702..6718
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 ggccggatcc cagtgtggtg gtacgtagga attcatggat cccgggcagc agccgccgcc
       61 tcaaccggcc ccccagggcc aagggcagcc gccttcgcag cccccgcagg ggcagggccc
      121 gccgtccgga cccgggcaac cggcacccgc ggcgacccag gcggcgccgc aggcaccccc
      181 cgccgggcat cagatcgtgc acgtccgcgg ggactcggag accgacctgg aggcgctctt
      241 caacgccgtc atgaacccca agacggccaa cgtgccccag accgtgccca tgaggctccg
      301 gaagctgccc gactccttct tcaagccgcc ggagcccaaa tcccactccc gacaggccag
      361 tactgatgca ggcactgcag gagccctgac tccacagcat gttcgagctc attcctctcc
      421 agcttctctg cagttgggag ctgtttctcc tgggacactg acccccactg gagtagtctc
      481 tggcccagca gctacaccca cagctcagca tcttcgacag tcttcttttg agatacctga
      541 tgatgtacct ctgccagcag gttgggagat ggcaaagaca tcttctggtc agagatactt
      601 cttaaatcac atcgatcaga caacaacatg gcaggacccc aggaaggcca tgctgtccca
      661 gatgaacgtc acagccccca ccagtccacc agtgcagcag aatatgatga actcggcttc
      721 aggtcctctt cctgatggat gggaacaagc catgactcag gatggagaaa tttactatat
      781 aaaccataag aacaagacca cctcttggct agacccaagg cttgaccctc gttttgccat
      841 gaaccagaga atcagtcaga gtgctccagt gaaacagcca ccacccctgg ctccccagag
      901 cccacaggga ggcgtcatgg gtggcagcaa ctccaaccag cagcaacaga tgcgactgca
      961 gcaactgcag atggagaagg agaggctgcg gctgaaacag caagaactgc ttcggcaggt
     1021 gaggccacag gcaatgcgga atatcaatcc cagcacagca aattctccaa aatgtcagga
     1081 gttagccctg cgtagccagt taccaacact ggagcaggat ggtgggactc aaaatccagt
     1141 gtcttctccc gggatgtctc aggaattgag aacaatgacg accaatagct cagatccttt
     1201 ccttaacagt ggcacctatc actctcgaga tgagagtaca gacagtggac taagcatgag
     1261 cagctacagt gtccctcgaa ccccagatga cttcctgaac agtgtggatg agatggatac
     1321 aggtgatact atcaaccaaa gcaccctgcc ctcacagcag aaccgtttcc cagactacct
     1381 tgaagccatt cctgggacaa atgtggacct tggaacactg gaaggagatg gaatgaacat
     1441 agaaggagag gagctgatgc caagtctgca ggaagctttg agttctgaca tccttaatga
     1501 catggagtct gttttggctg ccaccaagct agataaagaa agctttctta catggttata
     1561 ggtcgaccct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     1621 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     1681 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     1741 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     1801 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     1861 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttgccagct
     1921 ggggcgccct ctggtaaggt tgggaagccc tgcagatctg atcccggggg gcaatgagat
     1981 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     2041 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     2101 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     2161 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     2221 ggggaattta gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     2281 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     2341 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     2401 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     2461 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     2521 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     2581 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     2641 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     2701 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     2761 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     2821 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     2881 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     2941 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     3001 gaatagagta gatgccgacc gggatctatc gatgcggcgg gtgtggtggt tacgcgtaat
     3061 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3121 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3181 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3241 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3301 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3361 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3421 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3481 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3541 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3601 aggggggcgg agcctatgga aaaacgccag caacgcatcg ataaaataaa agattttatt
     3661 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta
     3721 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc
     3781 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     3841 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
     3901 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
     3961 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
     4021 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
     4081 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
     4141 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
     4201 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
     4261 cgggggtctt tcacatgcag catgtatcaa aattaatttg gttttttttc ttaagtattt
     4321 acattaaatg gccatagtgc ggccgcaaat caatctaaag tatatatgag taaacttggt
     4381 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4441 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4501 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4561 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4621 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4681 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4741 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4801 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4861 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4921 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4981 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5041 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5101 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5161 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5221 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5281 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5341 taggggttcc gcgcacattt ccccgaaaag tgccacctgc agcctgaata tgggccaaac
     5401 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
     5461 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     5521 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
     5581 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     5641 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     5701 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
     5761 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
     5821 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
     5881 cccctgccca gggaccaccg acccaccacc gggaggcaag ctggccagca acttatctgt
     5941 gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag
     6001 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga acacccggcc
     6061 gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg acctgaggaa
     6121 gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga gacgagaacc
     6181 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga agccgcgcgt
     6241 cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt
     6301 atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc ttaggtcact
     6361 ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg
     6421 ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct
     6481 ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac
     6541 acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct
     6601 gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc
     6661 tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc
     6721 cttctctagg cgcc
//
