LOCUS       sequence-240849-       11759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PDGFRA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PDGFRA
FEATURES             Location/Qualifiers
     source          1..11759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6719..6743)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6751..6769
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6794..6813)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6795..6813)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6819..6839)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6893..6934
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6955..7522
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6972..7524
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7116..7133)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7343..7362
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7536..8135
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7536..7555)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8032..8052
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8149..8737
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8202..8222)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8620..8631)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8812..9045
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9164..9182)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9250..9354
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9355..9423,9424..10215)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9573..9592)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10386..10974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10875..10894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11128..11145
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11385..11406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11421..11426,11427..11444,11445..11451)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11459..11475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11464..11486
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11483..11499
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11483..11499
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11549..11568
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gggacttccc atccggcgtt cctggtctta
     3481 ggctgtcttc tcacagggct gagcctaatc ctctgccagc tttcattacc ctctatcctt
     3541 ccaaatgaaa atgaaaaggt tgtgcagctg aattcatcct tttctctgag atgctttggg
     3601 gagagtgaag tgagctggca gtaccccatg tctgaagaag agagctccga tgtggaaatc
     3661 agaaatgaag aaaacaacag cggccttttt gtgacggtct tggaagtgag cagtgcctcg
     3721 gcggcccaca cagggttgta cacttgctat tacaaccaca ctcagacaga agagaatgag
     3781 cttgaaggca ggcacattta catctatgtg ccagacccag atgtagcctt tgtacctcta
     3841 ggaatgacgg attatttagt catcgtggag gatgatgatt ctgccattat accttgtcgc
     3901 acaactgatc ccgagactcc tgtaacctta cacaacagtg agggggtggt acctgcctcc
     3961 tacgacagca gacagggctt taatgggacc ttcactgtag ggccctatat ctgtgaggcc
     4021 accgtcaaag gaaagaagtt ccagaccatc ccatttaatg tttatgcttt aaaagcaaca
     4081 tcagagctgg atctagaaat ggaagctctt aaaaccgtgt ataagtcagg ggaaacgatt
     4141 gtggtcacct gtgctgtttt taacaatgag gtggttgacc ttcaatggac ttaccctgga
     4201 gaagtgaaag gcaaaggcat cacaatgctg gaagaaatca aagtcccatc catcaaattg
     4261 gtgtacactt tgacggtccc cgaggccacg gtgaaagaca gtggagatta cgaatgtgct
     4321 gcccgccagg ctaccaggga ggtcaaagaa atgaagaaag tcactatttc tgtccatgag
     4381 aaaggtttca ttgaaatcaa acccaccttc agccagttgg aagctgtcaa cctgcatgaa
     4441 gtcaaacatt ttgttgtaga ggtgcgggcc tacccacctc ccaggatatc ctggctgaaa
     4501 aacaatctga ctctgattga aaatctcact gagatcacca ctgatgtgga aaagattcag
     4561 gaaataaggt atcgaagcaa attaaagctg atccgtgcta aggaagaaga cagtggccat
     4621 tatactattg tagctcaaaa tgaagatgct gtgaagagct atacttttga actgttaact
     4681 caagttcctt catccattct ggacttggtc gatgatcacc atggctcaac tgggggacag
     4741 acggtgaggt gcacagctga aggcacgccg cttcctgata ttgagtggat gatatgcaaa
     4801 gatattaaga aatgtaataa tgaaacttcc tggactattt tggccaacaa tgtctcaaac
     4861 atcatcacgg agatccactc ccgagacagg agtaccgtgg agggccgtgt gactttcgcc
     4921 aaagtggagg agaccatcgc cgtgcgatgc ctggctaaga atctccttgg agctgagaac
     4981 cgagagctga agctggtggc tcccaccctg cgttctgaac tcacggtggc tgctgcagtc
     5041 ctggtgctgt tggtgattgt gatcatctca cttattgtcc tggttgtcat ttggaaacag
     5101 aaaccgaggt atgaaattcg ctggagggtc attgaatcaa tcagcccgga tggacatgaa
     5161 tatatttatg tggacccgat gcagctgcct tatgactcaa gatgggagtt tccaagagat
     5221 ggactagtgc ttggtcgggt cttggggtct ggagcgtttg ggaaggtggt tgaaggaaca
     5281 gcctatggat taagccggtc ccaacctgtc atgaaagttg cagtgaagat gctaaaaccc
     5341 acggccagat ccagtgaaaa acaagctctc atgtctgaac tgaagataat gactcacctg
     5401 gggccacatt tgaacattgt aaacttgctg ggagcctgca ccaagtcagg ccccatttac
     5461 atcatcacag agtattgctt ctatggagat ttggtcaact atttgcataa gaatagggat
     5521 agcttcctga gccaccaccc agagaagcca aagaaagagc tggatatctt tggattgaac
     5581 cctgctgatg aaagcacacg gagctatgtt attttatctt ttgaaaacaa tggtgactac
     5641 atggacatga agcaggctga tactacacag tatgtcccca tgctagaaag gaaagaggtt
     5701 tctaaatatt ccgacatcca gagatcactc tatgatcgtc cagcctcata taagaagaaa
     5761 tctatgttag actcagaagt caaaaacctc ctttcagatg ataactcaga aggccttact
     5821 ttattggatt tgttgagctt cacctatcaa gttgcccgag gaatggagtt tttggcttca
     5881 aaaaattgtg tccaccgtga tctggctgct cgcaacgtcc tcctggcaca aggaaaaatt
     5941 gtgaagatct gtgactttgg cctggccaga gacatcatgc atgattcgaa ctatgtgtcg
     6001 aaaggcagta cctttctgcc cgtgaagtgg atggctcctg agagcatctt tgacaacctc
     6061 tacaccacac tgagtgatgt ctggtcttat ggcattctgc tctgggagat cttttccctt
     6121 ggtggcaccc cttaccccgg catgatggtg gattctactt tctacaataa gatcaagagt
     6181 gggtaccgga tggccaagcc tgaccacgct accagtgaag tctacgagat catggtgaaa
     6241 tgctggaaca gtgagccgga gaagagaccc tccttttacc acctgagtga gattgtggag
     6301 aatctgctgc ctggacaata taaaaagagt tatgaaaaaa ttcacctgga cttcctgaag
     6361 agtgaccatc ctgctgtggc acgcatgcgt gtggactcag acaatgcata cattggtgtc
     6421 acctacaaaa acgaggaaga caagctgaag gactgggagg gtggtctgga tgagcagaga
     6481 ctgagcgctg acagtggcta catcattcct ctgcctgaca ttgaccctgt ccctgaggag
     6541 gaggacctgg gcaagaggaa cagacacagc tcgcagacct ctgaagagag tgccattgag
     6601 acgggttcca gcagttccac cttcatcaag agagaggacg agaccattga agacatcgac
     6661 atgatggacg acatcggcat agactcttca gacctggtgg aagacagctt cctgtagaac
     6721 ccagctttct tgtacaaagt ggtgatatcc aattaaccct cactaaagga ttgatttgtt
     6781 gaaataagtg ttgccctata gtgagtcgta ttaccaccca acttttctat acaaagtggt
     6841 tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc
     6901 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagatccctc
     6961 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     7021 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     7081 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     7141 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     7201 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     7261 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     7321 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     7381 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     7441 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     7501 cctttgaaaa acacgatgat aatatggcca cacatatgac cgagtacaag cccacggtgc
     7561 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     7621 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     7681 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     7741 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     7801 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     7861 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     7921 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     7981 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     8041 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     8101 gcacctggtg catgacccgc aagcccggtg cctaaatcga tagatcctaa tcaacctctg
     8161 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     8221 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     8281 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     8341 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     8401 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     8461 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8521 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     8581 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8641 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8701 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga
     8761 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8821 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     8881 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8941 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9001 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     9061 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg
     9121 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta
     9181 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     9241 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     9301 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     9361 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     9421 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     9481 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     9541 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     9601 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     9661 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     9721 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     9781 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     9841 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     9901 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     9961 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    10021 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    10081 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    10141 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    10201 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    10261 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    10321 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10381 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    10441 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    10501 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    10561 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    10621 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10681 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    10741 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    10801 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    10861 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10921 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10981 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    11041 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    11101 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    11161 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg
    11221 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    11281 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac
    11341 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    11401 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    11461 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat
    11521 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    11581 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    11641 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    11701 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
