LOCUS       sequence-24103-e        1109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1109)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme S44A WT*
FEATURES             Location/Qualifiers
     source          1..1109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    723..739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(747..753,754..769,770..775))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    818..834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(842..848,849..864,865..870))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 tncgaaaaag cccgctcatt aggcgggctc agatctgctc atgttttgac agcttatcat
       61 cgataagctt cggcaataaa ttcttgatat gttttcatat gtttccttta aatattttaa
      121 ttagtaattg tctattcaag taattgtgaa tatactatca caattccaag agaaagtaaa
      181 cagctttata gatttttata cgcgtcccaa gtgccagttc taaacgttgt aatgactcgt
      241 tttgcgcgat taggtgtttg attataccat ctacttttag ctaagttaac tgctgcttca
      301 tcccagcgtt tttgttgaag catacgtaaa gagttagtaa atcctgccac accggtttct
      361 cccatttgga aaaccatatt aatcaatgca gcgcgacgaa ccgcatcaag agaatcataa
      421 accggtttta atttagcatt tctcagaatt ccgcgaacag cagcatcaac atcctgatta
      481 aagagttttt cagcctcatc ttttgtaatt acaccattag tattacgccc aatagcttta
      541 tctaattccg ctttagcagc attaagtgat ggactttttg taagcaaatg accgatgcca
      601 atagtgtaat agccttctgt gtctttatag attttaagtc taagaccttc atctatacgt
      661 aacatttcaa atatattcat aatacctcct aagcatcgat ggatcctgtt tcctgtgtga
      721 aattgttatc cgctcacaat tccacacatt atacgagccg atgattaatt gtcaacaggg
      781 ggatggggag taagctgatc ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      841 acattatacg agccgatgat taattgtcaa caggggggat gggggagtaa gctcatcgat
      901 ggatcgatcn gggttncnng tgtgaaattg ntatccgctc ncaattccnc acattanacg
      961 aggccgggaa gccntaaagt gnaaagccng gggggngccc aaaggngtga gcctaactta
     1021 cattnaattg nngttggcgg ctcaccngcc cgctttccaa tcgggnaaac ctggtcgggg
     1081 ccaggnaccc catcgnaagg gggncnaaa
//
