LOCUS       sequence-241234-         466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-30d-3p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-30d-3p
FEATURES             Location/Qualifiers
     source          1..466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(57..81)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(305..324)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    370..425
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(424..443)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 cagatactgc gacctcccta gcaaactggg gcacaaactt aattgagaat tctaccaccc
       61 agctttcttg tacaaagtgg tacgtctcaa gaccagaggt ctctgcttga cgcggcgcag
      121 caaacatctg actgaaaggc agcaaacatc tgactgaaag gcagcaaaca tctgactgaa
      181 aggcagcaaa catctgactg aaagcaacca gaagagacct gtgcttcact cctcaggtgc
      241 aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact
      301 gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga
      361 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
      421 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatca
//
