LOCUS       sequence-241283-        4092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate (MoClo; CDS1) compatible luciferase gene from 
            Photinuspyralis with an intron for transient and stable expression 
            in plants .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, 
            Lee-Parsons CWT
  TITLE     EASI Transformation: An Efficient Transient Expression Method for 
            Analyzing Gene Function in Catharanthus roseus Seedlings.
  JOURNAL   Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. 
            eCollection 2019.
  PUBMED    31263474
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-06-11"; volume: "10:755. doi"; pages: " 
            10.3389/fpls.2019.00755. eCollection 2019"
COMMENT     Sequence Label: pSB63 - pL0_fLUC-I (CDS1)
FEATURES             Location/Qualifiers
     source          1..4092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(116..134)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          194..382
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     primer_bind     complement(1931..1949)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2017..2121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2343..3134
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3305..3893
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3794..3813
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4047..4064
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatcactctg tggtctcaaa tggaggacgc
       61 caaaaacata aagaaaggcc cggcgccatt ctatcctcta gaggatggaa ccgctggaga
      121 gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga
      181 tgcacatatc gaggtaagtt tctgcttcta cctttgatat atatataata attatcatta
      241 attagtagta atataatatt tcaaatattt ttttcaaaat aaaagaatgt agtatatagc
      301 aattgctttt ctgtagttta taagtgtgta tattttaatt tataactttt ctaatatatg
      361 accaaaattt gttgatgtgc aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt
      421 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
      481 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
      541 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgaacatttc
      601 gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
      661 aaaattacca ataatccaga aaattattat catggattct aaaacggatt accagggatt
      721 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
      781 tgtaccagag tcctttgatc gtgacaaaac aattgcactg ataatgaatt cctctggatc
      841 tactgggtta cctaagggtg tggcccttcc gcatagaact gcctgcgtca gattctcgca
      901 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt
      961 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg
     1021 agtcgtctta atgtatagat ttgaagaaga gctgttttta cgatcccttc aggattacaa
     1081 aattcaaagt gcgttgctag taccaaccct attttcattc ttcgccaaaa gcactctgat
     1141 tgacaaatac gatttatcta atttacacga aattgcttct gggggcgcac ctctttcgaa
     1201 agaagtcggg gaagcggttg caaaacgctt ccatcttcca gggatacgac aaggatatgg
     1261 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc
     1321 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac
     1381 gctgggcgtt aatcagagag gcgaattatg tgtcagagga cctatgatta tgtccggtta
     1441 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg
     1501 agacatagct tactgggacg aggacgaaca cttcttcata gttgaccgct tgaagtcttt
     1561 aattaaatac aaaggatatc aggtggcccc cgctgaattg gaatcgatat tgttacaaca
     1621 ccccaacatc ttcgacgcgg gcgtggcagg tctgcccgac gatgacgccg gtgaacttcc
     1681 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta
     1741 cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
     1801 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
     1861 ggccaagaag ggcggaaagt ccaaattgta agctttgaga ccacgaagtg gctcttcagt
     1921 ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1981 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2041 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2101 taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     2161 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttttgggg
     2221 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     2281 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     2341 tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     2401 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     2461 gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta aggcttgatg
     2521 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     2581 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2641 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2701 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2761 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2821 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2881 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2941 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     3001 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     3061 cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag atcaccaagg
     3121 tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     3181 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3241 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3301 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3361 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3421 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3481 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3541 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3601 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3661 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3721 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3781 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3841 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3901 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3961 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4021 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4081 caatacgcaa ac
//
