LOCUS       sequence-24132-e        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme K48-[HP] WT*
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    731..747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(755..761,762..777,778..783))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    826..842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(850..856,857..872,873..878))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     gap             1121..1130
                     /estimated_length=10
ORIGIN
        1 gctantggaa aagcccgctc attaggcggg ctcagatctg ctcatgtttg acagcttatc
       61 atcgataagc ttcggcaata aattcttgat atgttttcat atgtttcctt taaatatttt
      121 aattagtaat tgtctattca agtaattgtg aatatactat cacaattcca agagaaagta
      181 aacagcttta tagattttta tacgcgtccc aagtgccagt tctaaacgtt gtaatgactc
      241 gttttgcgcg attaggtgtt tgattatacc atctactttt agctaagtta actgctgctt
      301 catcccagcg tttttgttga agcatacgta aagagttagt aaatcctgcc acaccggttt
      361 ctcccatttg gaaaaccata ttaatcaatg cagcgcgacg aaccgcatca agagaatcat
      421 aaaccggttt taatttagca tttctcagaa ttccgcgaac agcagcatca acatcctgat
      481 taaagagttt ttcagcctca tcttttgtaa ttacaccatt agtattacgc ccaatagctg
      541 ggtgtttatc taattcagat ttagcagcat taagtgatgg actttttgta agcaaatgac
      601 cgatgccaat agtgtaatag ccttctgtgt ctttatagat tttaagtcta agaccttcat
      661 ctatacgtaa catttcaaat atattcataa tacctcctaa gcatcgatgg atcctgtttc
      721 ctgtgtgaaa ttgttatccg ctcacaattc cacacattat acgagccgat gattaattgt
      781 caacaggggg atggggagta agctgatcct gtttcctgtg tgaaattgtt atccgctcac
      841 aattccacac attatacgag ccgatganta attgtcaaca gggggatggg gagtaagctc
      901 atcgatggat cgatccggnt tnccngngng aaantggnan ccgcncncaa ttccccncnt
      961 tanncnancc ggaancnnan ngggnaaanc ccgggggggc cnanngnngg nncnaanntn
     1021 cnnnnnntng gnnngnnnnn cnnggnccnn nnttnccnnn ngggaaaccg gncngggcnn
     1081 ggnnnccnnn nnnnnggggg nnaanccntt nnggnnnngg nnnnnnnnnn ccc
//
