LOCUS       sequence-241445-        1840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  green fluorescent glucose sensor for mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Mita M, Ito M, Harada K, Sugawara I, Ueda H, Tsuboi T, Kitaguchi T
  TITLE     Green Fluorescent Protein-Based Glucose Indicators Report Glucose 
            Dynamics in Living Cells.
  JOURNAL   Anal Chem. 2019 Apr 2;91(7):4821-4830. doi: 
            10.1021/acs.analchem.9b00447. Epub 2019 Mar 22.
  PUBMED    30869867
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.analchem.9b00447"; journalName: "Anal Chem"; date: 
            "2019-04-2- 2"; volume: "91"; issue: "7"; pages: "4821-4830"
COMMENT     Sequence Label: Green Glifon4000
FEATURES             Location/Qualifiers
     source          1..1840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(112..133)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(373..392)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1465..1716
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1653..1674
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1750..1767)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 attaatacga ctcactatag ggagacccaa gctggctagc gtttaaacgg gccctctaga
       61 ctcgagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      121 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      181 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      241 cccaccctcg tgaccacctt cggctacggc ctgatgtgct tcgcccgcta ccccgaccac
      301 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      361 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      421 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      481 gggcacaagc tggagtttgg tctcctggga gttgatacta gcattggtgt aacaatctat
      541 aagtacgacg ataacttgat gtctgtagtg cgcaaggcta ttgagcaaga tgcgaaagcc
      601 gcgccagatg ttcagctgct gatgaatgat tctcagaatg accagtccaa gcagaacgat
      661 cagatcgacg tattgctggc gaaaggggtg aaggcactgg caatcaacct ggttgacccg
      721 gcagctgcgg gtacggtgat tgagaaagcg cgtgggcaaa acgtgccggt ggttttcttc
      781 aacaaagaac cgtctcgtaa ggcgctggat agctacgaca aagcctacta cgttggcact
      841 gactccaaag agtccggcat tattcaaggc gatttgattg ctaaacactg ggcggcgaat
      901 cagggttggg atctgaacaa agacggtcag attcagttcg tactgctgaa aggtgaaccg
      961 ggccatccgg atgcagaagc acgtaccact tacgtgatta aagaattgaa cgataaaggc
     1021 atcaaaactg aacagttaca gttagatacc gcaatgtggg acaccgctca ggcgaaagat
     1081 aagatggacg cctggctgtc tggcccgaac gccaacaaaa tcgaagtggt tatcgccaac
     1141 aacgatgcga tggcaatggg cgcggttgaa gcgctgaaag cacacaacaa gtccagcatt
     1201 ccggtgtttg gcgtcgatgc gctgccagaa gcgctggcgc tggtgaaatc cggtgcactg
     1261 gcgggcaccg tactgaacga tgctaacaac caggcgaaag cgacctttga tctggcgaaa
     1321 aacctggccg atggtaaagg tgcggctgat ggcaccaact ggaaaatcga caacaaagtg
     1381 gtccgcgtac cttatgttgg cgtagataaa gacaacctgg ctgagttcag cgaggtggat
     1441 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1501 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1561 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc
     1621 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1681 gccgggatca ctctcggcat ggacgagctg tacaagtaat tcgaagctta agtttaaacc
     1741 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     1801 tgccttcctt gaccctggaa ggtgccactc ccactgtcct
//
