LOCUS       sequence-241457-        4301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector Backbone for his-24 fusion and unc-54 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4301)
  TITLE     Leifer Lab worm expression plasmids
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAS1-his-24
FEATURES             Location/Qualifiers
     source          1..4301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..68
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     60..77
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     61..77
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     87..106
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        87..105
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1574..1594)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1574..1592)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1613..1629)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1613..1629)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1626..1648)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1637..1653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1661..1667,1668..1685,1686..1691))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1706..1727
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1844..1861)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2015..2603)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2095..2114)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2774..3565,3566..3634))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3397..3416
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3635..3739)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3765..4220)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3902..3923)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4114..4133
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
       61 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggagctcc
      121 accgcggact agtggatccc ccgggctgca gcctaggatg tctgattccg ctgttgttgc
      181 cgccgctgtc gagccaaagg tcccaaaggc taaggccgcc aaggccgcca agccagccgc
      241 caaggccgcc aagccaacca aagttgccaa ggccaaggct ccagtcgctc acccaccata
      301 catcaacatg atcaaggagg ccatcaagca gctcaaggac cgcaaaggag cttccaagca
      361 ggcaatcctc aagttcatct cccaaaacta caagctcgga gacaatgtca tccagatcaa
      421 tgctcatctc cgtcaggctc tcaagcgtgg tgtcaccagc aaggcccttg ttcaagccgc
      481 cggatccgga gccaacggac gtttccgtgt gccagagaag gccgccgccg ccaagaagcc
      541 agcagcagcc aagaagccag cagcagccaa gaaaccagca gccgccaaga aagccactgg
      601 agagaagaag gcaaagaagc cagccgctgc caagccaaag aaggccgcca ccggagataa
      661 gaaggtcaag aaggccaaga gcccaaagaa ggttgccaag ccagctgcca agaaggtcgc
      721 caagtctcca gccaagaagg ccgctccaaa gaagatcgcc aagccagctg ccaagaaggc
      781 cgccaagcca gccgccaagg ccgcatgcaa gctttggaat tcatctcgcg cccgtgcctc
      841 tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca
      901 ccccctattt ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt
      961 taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa
     1021 agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac
     1081 aatgttctgt gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata
     1141 gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt
     1201 ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt
     1261 ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt
     1321 ttgctttttg gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct
     1381 aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag
     1441 gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt
     1501 ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttg ggcccggtac
     1561 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     1621 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccgggagca
     1681 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     1741 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1801 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1861 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1921 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1981 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2041 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2101 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2161 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2221 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2281 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2341 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2401 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2461 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2521 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2581 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2641 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2701 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2761 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgcctat
     2821 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2881 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     2941 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3001 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3061 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3121 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3181 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3241 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3301 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3361 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3421 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3481 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3541 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3601 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3661 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3721 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
     3781 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     3841 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     3901 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     3961 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     4021 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     4081 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     4141 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     4201 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
     4261 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa g
//
