LOCUS       sequence-241491-       12428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of RAD50.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12428)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-RAD50
FEATURES             Location/Qualifiers
     source          1..12428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7388..7412)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7420..7438
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7463..7482)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7464..7482)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7488..7508)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7562..7603
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7624..8191
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7641..8193
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7785..7802)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8012..8031
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8205..8804
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8205..8224)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8701..8721
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8818..9406
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8871..8891)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9289..9300)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9481..9714
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9833..9851)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9919..10023
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10024..10092,10093..10884)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10242..10261)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11055..11643
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11544..11563
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11797..11814
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12054..12075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12090..12095,12096..12113,12114..12120)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12128..12144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12133..12155
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12152..12168
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12152..12168
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12218..12237
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcttcatg tcccggatcg aaaagatgag cattctgggc
     3481 gtgcggagtt ttggaataga ggacaaagat aagcaaatta tcactttctt cagccccctt
     3541 acaattttgg ttggacccaa tggggcggga aagacgacca tcattgaatg tctaaaatat
     3601 atttgtactg gagatttccc tcctggaacc aaaggaaata catttgtaca cgatcccaag
     3661 gttgctcaag aaacagatgt gagagcccag attcgtctgc aatttcgtga tgtcaatgga
     3721 gaacttatag ctgtgcaaag atctatggtg tgtactcaga aaagcaaaaa gacagaattt
     3781 aaaactctgg aaggagtcat tactagaaca aagcatggtg aaaaggtcag tctgagctct
     3841 aagtgtgcag aaattgaccg agaaatgatc agttctcttg gggtttccaa ggctgtgcta
     3901 aataatgtca ttttctgtca tcaagaagat tctaattggc ctttaagtga aggaaaggct
     3961 ttgaagcaaa agtttgatga gattttttca gcaacaagat acattaaagc cttagaaaca
     4021 cttcggcagg tacgtcagac acaaggtcag aaagtaaaag aatatcaaat ggaactaaaa
     4081 tatctgaagc aatataagga aaaagcttgt gagattcgtg atcagattac aagtaaggaa
     4141 gcccagttaa catcttcaaa ggaaattgtc aaatcctatg agaatgaact tgatccattg
     4201 aagaatcgtc taaaagaaat tgaacataat ctctctaaaa taatgaaact tgacaatgaa
     4261 attaaagcct tggatagccg aaagaagcaa atggagaaag ataatagtga actggaagag
     4321 aaaatggaaa aggtttttca agggactgat gagcaactaa atgacttata tcacaatcac
     4381 cagagaacag taagggagaa agaaaggaaa ttggtagact gtcatcgtga actggaaaaa
     4441 ctaaataaag aatctaggct tctcaatcag gaaaaatcag aactgcttgt tgaacagggt
     4501 cgtctacagc tgcaagcaga tcgccatcaa gaacatatcc gagctagaga ttcattaatt
     4561 cagtctttgg caacacagct agaattggat ggctttgagc gtggaccatt cagtgaaaga
     4621 cagattaaaa attttcacaa acttgtgaga gagagacaag aaggggaagc aaaaactgcc
     4681 aaccaactga tgaatgactt tgcagaaaaa gagactctga aacaaaaaca gatagatgag
     4741 ataagagata agaaaactgg actgggaaga ataattgagt taaaatcaga aatcctaagt
     4801 aagaagcaga atgagctgaa aaatgtgaag tatgaattac agcagttgga aggatcttca
     4861 gacaggattc ttgaactgga ccaggagctc ataaaagctg aacgtgagtt aagcaaggct
     4921 gagaaaaaca gcaatgtaga aaccttaaaa atggaagtaa taagtctcca aaatgaaaaa
     4981 gcagacttag acaggaccct gcgtaaactt gaccaggaga tggagcagtt aaaccatcat
     5041 acaacaacac gtacccaaat ggagatgctg accaaagaca aagctgacaa agatgaacaa
     5101 atcagaaaaa taaaatctag gcacagtgat gaattaacct cactgttggg atattttccc
     5161 aacaaaaaac agcttgaaga ctggctacat agtaaatcaa aagaaattaa tcagaccagg
     5221 gacagacttg ccaaattgaa caaggaacta gcttcatctg agcagaataa aaatcatata
     5281 aataatgaac taaaaagaaa ggaagagcag ttgtccagtt acgaagacaa gctgtttgat
     5341 gtttgtggta gccaggattt tgaaagtgat ttagacaggc ttaaagagga aattgaaaaa
     5401 tcatcaaaac agcgagccat gctggctgga gccacagcag tttactccca gttcattact
     5461 cagctaacag acgaaaacca gtcatgttgc cccgtttgtc agagagtttt tcagacagag
     5521 gctgagttac aagaagtcat cagtgatttg cagtctaaac tgcgacttgc tccagataaa
     5581 ctcaagtcaa cagaatcaga gctaaaaaaa aaggaaaagc ggcgtgatga aatgctggga
     5641 cttgtgccca tgaggcaaag cataattgat ttgaaggaga aggaaatacc agaattaaga
     5701 aacaaactgc agaatgtcaa tagagacata cagcgcctaa agaacgacat agaagaacaa
     5761 gaaacactct tgggtacaat aatgcctgaa gaagaaagtg ccaaagtatg cctgacagat
     5821 gttacaatta tggagaggtt ccagatggaa cttaaagatg ttgaaagaaa aattgcacaa
     5881 caagcagcta agctacaagg aatagactta gatcgaactg tccaacaagt caaccaggag
     5941 aaacaagaga aacagcacaa gttagacaca gtttctagta agattgaatt gaatcgtaag
     6001 cttatacagg accagcagga acagattcaa catctaaaaa gtacaacaaa tgagctaaaa
     6061 tctgagaaac ttcagatatc cactaatttg caacgtcgtc agcaactgga ggagcagact
     6121 gtggaattat ccactgaagt tcagtctttg tacagagaga taaaggatgc taaagagcag
     6181 gtaagccctt tggaaacaac attggaaaag ttccagcaag aaaaagaaga attaatcaac
     6241 aaaaaaaata caagcaacaa aatagcacag gataaactga atgatattaa agagaaggtt
     6301 aaaaatattc atggctatat gaaagacatt gagaattata ttcaagatgg gaaagacgac
     6361 tataagaagc aaaaagaaac tgaacttaat aaagtaatag ctcaactaag tgaatgcgag
     6421 aaacacaaag aaaagataaa tgaagatatg agactcatga gacaagatat tgatacacag
     6481 aagatacaag aaaggtggct acaagataac cttactttaa gaaaaagaaa tgaggaacta
     6541 aaagaagttg aagaagaaag aaaacaacat ttgaaggaaa tgggtcaaat gcaggttttg
     6601 caaatgaaaa gtgaacatca gaagttggaa gagaacatag acaatataaa aagaaatcat
     6661 aatttggcat tagggcgaca gaaaggttat gaagaagaaa ttattcattt taagaaagaa
     6721 cttcgagaac cacaatttcg ggatgctgag gaaaagtata gagaaatgat gattgttatg
     6781 aggacaacag aacttgtgaa caaggatctg gatatttatt ataagactct tgaccaagca
     6841 ataatgaaat ttcacagtat gaaaatggaa gaaatcaata aaattatacg tgacctgtgg
     6901 cgaagtacct atcgtggaca agatattgaa tacatagaaa tacggtctga tgccgatgaa
     6961 aatgtatcag cttctgataa aaggcggaat tataactacc gagtggtgat gctgaaggga
     7021 gacacagcct tggatatgcg aggacgatgc agtgctggac aaaaggtatt agcctcactc
     7081 atcattcgcc tggccctggc tgaaacgttc tgcctcaact gtggcatcat tgccttggat
     7141 gagccaacaa caaatcttga ccgagaaaac attgaatctc ttgcacatgc tctggttgag
     7201 ataataaaaa gtcgctcaca gcagcgtaac ttccagcttc tggtaatcac tcatgatgaa
     7261 gattttgtgg agcttttagg acgttctgaa tatgtggaga aattctacag gattaaaaag
     7321 aacatcgatc agtgctcaga gattgtgaaa tgcagtgtta gctccctggg attcaatgtt
     7381 cattaagacc cagctttctt gtacaaagtg gtgatatcca attaaccctc actaaaggtg
     7441 agtaaatagt ttatgagtag taccctatag tgagtcgtat taccacccaa cttttctata
     7501 caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga
     7561 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg
     7621 agatccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     7681 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     7741 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     7801 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     7861 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     7921 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     7981 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     8041 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     8101 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     8161 cgtggttttc ctttgaaaaa cacgatgata atatggccac acatatgacc gagtacaagc
     8221 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     8281 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     8341 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     8401 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     8461 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     8521 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     8581 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     8641 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     8701 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     8761 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctaaatcgat agatcctaat
     8821 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8881 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8941 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     9001 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     9061 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     9121 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     9181 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     9241 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     9301 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     9361 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa
     9421 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     9481 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     9541 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     9601 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     9661 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     9721 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     9781 agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg
     9841 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     9901 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     9961 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    10021 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    10081 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    10141 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    10201 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    10261 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    10321 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    10381 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    10441 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    10501 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    10561 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    10621 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    10681 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    10741 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    10801 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    10861 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    10921 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    10981 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    11041 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    11101 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    11161 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    11221 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    11281 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    11341 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    11401 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    11461 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    11521 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    11581 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    11641 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    11701 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    11761 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    11821 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    11881 aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    11941 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
    12001 ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    12061 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    12121 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
    12181 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    12241 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
    12301 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
    12361 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
    12421 ttagtagt
//
