LOCUS       sequence-241742-       12367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of IKBKB__ANK1 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12367)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-IKBKB__ANK1
FEATURES             Location/Qualifiers
     source          1..12367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3550..3574)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7427..7447)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7501..7542
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7563..8130
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7580..8132
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7724..7741)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7951..7970
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8144..8743
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8144..8163)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8640..8660
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8757..9345
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8810..8830)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9228..9239)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9420..9653
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9772..9790)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9858..9962
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9963..10031,10032..10823)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10181..10200)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10994..11582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11483..11502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11736..11753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11993..12014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12029..12034,12035..12052,12053..12059)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12067..12083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12072..12094
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12091..12107
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12091..12107
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12157..12176
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatgagc tggtcacctt ccctgacaac gcagacatgt
     3481 ggggcctggg aaatgaaaga gcgccttggg acagggggat ttggaaatgt catccgatgg
     3541 cacaatcagt cccagctttc ttgtacaaag tggttcaatg gctacacccc tttgcacatc
     3601 gctgccaagc agaaccaggt ggaggtggcc cgtagtctgc tgcagtatgg gggctcagca
     3661 aacgccgagt cggtgcaagg tgtgacgccc cttcacctgg ccgcccagga gggccacgca
     3721 gagatggtgg ctctgctgct ctcgaaacaa gccaatggca acctggggaa caagagcgga
     3781 ctcactcccc tccatctggt agcacaagaa ggccacgttc cagtggcaga tgtgctgatc
     3841 aaacacggcg tcatggtgga cgccaccacc cggatgggct acactcccct ccatgtggcc
     3901 agtcactatg gaaacatcaa gctggtgaag tttctgctgc agcaccaggc agatgtcaat
     3961 gccaagacca agctaggata cagccccctg caccaggcag cccagcaggg acacacagac
     4021 atcgtgactc tgcttctgaa aaacggtgct tccccaaacg aggtcagctc ggatggaacc
     4081 acacctctgg ccatagccaa gcgcttgggc tacatttctg tcaccgacgt gctcaaggtc
     4141 gtcacggatg aaaccagttt cgtgttagtc agtgataagc atcgaatgag tttccctgag
     4201 acagttgatg agatcctgga tgtctcggaa gatgaagggg aagaactcat cagcttcaag
     4261 gctgagaggc gggattccag ggatgttgat gaagagaagg agctgctgga ttttgtgccg
     4321 aagctagacc aagtggtgga atctccagcc atccccagga ttccctgtgc catgcctgag
     4381 acagtggtga tcaggtcaga agagcaggag caggcatcta aagagtatga tgaggactcc
     4441 ctcatcccca gcagcccggc caccgagacc tcagacaaca tcagcccggt ggccagcccg
     4501 gtgcatacag ggtttctggt gagcttcatg gttgacgccc ggggtggttc catgagagga
     4561 agtcgccaca acggcctgcg agtggtgatc ccgccacgga cgtgcgcagc gcccacccgc
     4621 atcacctgcc gcctggtcaa gccccagaag ctcagcacgc cgcccccact ggccgaggag
     4681 gagggcctgg ccagcaggat catagcactg gggcccacgg gggcacagtt cctgagccct
     4741 gtaatcgtgg agatcccgca ctttgcctcc catggccgtg gagaccgcga gctcgtggtt
     4801 ctgaggagcg aaaacggctc cgtgtggaag gagcacagga gccgctatgg agagagctac
     4861 ctggatcaga tcctcaacgg gatggacgaa gagctgggga gcctggagga gctagagaag
     4921 aagagggtgt gccgaatcat caccaccgac ttcccgctgt acttcgtgat catgtcacgg
     4981 ctctgccagg actacgacac catcggtccc gaagggggct ccctgaagag caagctggtg
     5041 cccctggtac aggcaacgtt cccggagaat gccgtcacca agagagtgaa gctggctctg
     5101 caggcccagc ctgtcccgga tgagcttgtc actaagctcc tgggcaacca ggccacattc
     5161 agccccattg tcaccgtgga gccccggcgc cggaagttcc accgccccat tgggcttcgg
     5221 atcccactac ctccttcctg gaccgacaac ccgagggaca gcggggaggg agacaccacc
     5281 agcctgcgcc tgctttgcag cgtcattgga ggaacagacc aagcccagtg ggaagacata
     5341 acaggaacca ccaaacttgt atatgccaac gagtgcgcca acttcaccac caatgtctct
     5401 gccaggtttt ggctgtcgga ctgtcctcgg actgctgagg ctgtgaactt tgccaccctg
     5461 ctgtacaaag agctcactgc agtgccctac atggccaaat tcgtcatctt tgccaagatg
     5521 aatgaccccc gagaggggcg cctgcgctgc tactgcatga cagatgataa agtggacaag
     5581 accctggagc agcatgagaa cttcgtggag gtggcccgga gcagggacat agaggtgttg
     5641 gaaggaatgt ccctgtttgc agaactctct gggaacctgg tgcctgtgaa gaaagctgcc
     5701 cagcagcgga gcttccactt ccagtcattt cgggagaacc gtctggccat gcctgtaaag
     5761 gtgagggaca gcagtcgaga gccgggaggg tccctgtcgt ttctgcgcaa ggcgatgaag
     5821 tacgaggaca cccagcacat tctctgccac ctgaacatca ccatgccccc ctgcgccaag
     5881 ggaagtggag ccgaagatag gagaaggacc ccgacgcccc tggccctgcg atacagcatt
     5941 ctcagtgagt ccacaccagg ttctctcagt gggacagagc aggcagagat gaagatggct
     6001 gttatctcag agcacctcgg tctcagctgg gcagagttgg cccgggagct gcagttcagt
     6061 gtggaagaca tcaacaggat ccgagtggaa aatcccaact ccctgttgga gcagagtgtg
     6121 gccttgctga acctctgggt catccgtgaa ggccaaaacg caaacatgga gaatctgtac
     6181 acagccctgc agagcattga ccgtggcgag atcgtgaaca tgctggaggg ttccggccga
     6241 cagagccgca acttgaagcc agacaggcgg cacaccgacc gcgactactc gctgtcaccc
     6301 tcccagatga atggttactc ctcactgcag gacgagctgc tgtcccctgc ctccctgggc
     6361 tgtgcacttt cctctccgct acgtgcagac cagtactgga atgaggtggc cgtcctagac
     6421 gccatcccct tggcggccac ggagcatgac accatgctgg agatgtctga catgcaggtg
     6481 tggtctgcgg gcctcacgcc ttctctggtc actgctgagg actcctctct ggagtgtagc
     6541 aaggctgagg actctgatgc cacaggtcac gagtggaagt tggagggggc actctcagag
     6601 gaaccgcggg gccccgagtt gggctctctg gaacttgtgg aggacgacac agtggattca
     6661 gatgccacaa atggccttat cgatttgctt gaacaggagg aaggtcagag gtcagaagag
     6721 aagctgccag gttctaagag gcaggatgac gcgacaggtg cagggcagga ctcagagaat
     6781 gaagtgtctc ttgtttcagg ccatcagagg gggcaagccc gaatcacaca ttcccccacc
     6841 gtgagtcagg tgacggagag gagtcaggac agactgcagg actgggatgc agacggctcg
     6901 attgtctcat acctgcaaga tgctgcacaa ggttcctggc aagaggaggt cacgcaaggt
     6961 ccacactcat tccagggaac aagtaccatg actgaagggc tagagcccgg tggatctcag
     7021 gagtacgaga aggtcctggt gtctgtaagt gagcacacgt ggacagaaca gcccgaggct
     7081 gagagctccc aggccgacag ggaccggagg cagcaaggcc aagaagagca ggtgcaggag
     7141 gccaagaaca ccttcaccca agtggtgcag gggaatgagt ttcagaatat tccaggggag
     7201 caggtgacag aggagcaatt cacggatgag cagggcaaca ttgtcaccaa gaagatcatt
     7261 cgcaaggtgg ttcgacagat agacttgtcc agcgccgatg ccgcccagga gcacgaggag
     7321 gtgactgtag aggggcccct ggaggatccc agtgagctgg aggtcgatat tgattacttt
     7381 atgaaacact ccaaggatca cacctcgaca cccaacccct gacacccaac ttttctatac
     7441 aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa
     7501 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga
     7561 gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     7621 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     7681 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     7741 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     7801 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     7861 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     7921 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     7981 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     8041 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     8101 gtggttttcc tttgaaaaac acgatgataa tatggccaca catatgaccg agtacaagcc
     8161 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     8221 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     8281 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     8341 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     8401 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     8461 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     8521 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     8581 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     8641 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     8701 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc taaatcgata gatcctaatc
     8761 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     8821 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     8881 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     8941 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     9001 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     9061 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     9121 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     9181 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     9241 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     9301 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag
     9361 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     9421 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     9481 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     9541 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9601 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     9661 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     9721 gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga
     9781 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     9841 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9901 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9961 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    10021 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    10081 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    10141 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    10201 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    10261 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    10321 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    10381 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    10441 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    10501 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    10561 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    10621 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10681 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10741 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10801 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    10861 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    10921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    11041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    11101 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    11161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    11221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    11281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    11341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    11401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    11461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    11521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    11581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    11641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    11701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    11761 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca
    11821 agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    11881 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
    11941 cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    12001 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    12061 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt
    12121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    12181 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
    12241 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
    12301 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
    12361 tagtagt
//
