LOCUS       sequence-241875-        1851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion 
            of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT
            core (aa1048-1664), and Flag tag epitope driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, 
            Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, 
            Beisel CL, Barrangou R, Gersbach CA
  TITLE     Targeted transcriptional modulation with type I CRISPR-Cas systems 
            in human cells.
  JOURNAL   Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7.
  PUBMED    31548729
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7."
COMMENT     Sequence Label: pcDNA-LmoCas6-p300
FEATURES             Location/Qualifiers
     source          1..1851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1851
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      1615..1624
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 attttcaaac cagaagaact acgacaggca ctgatgccaa ctttggaggc actttaccgt
       61 caggatccag aatcccttcc ctttcgtcaa cctgtggacc ctcagctttt aggaatccct
      121 gattactttg atattgtgaa gagccccatg gatctttcta ccattaagag gaagttagac
      181 actggacagt atcaggagcc ctggcagtat gtcgatgata tttggcttat gttcaataat
      241 gcctggttat ataaccggaa aacatcacgg gtatacaaat actgctccaa gctctctgag
      301 gtctttgaac aagaaattga cccagtgatg caaagccttg gatactgttg tggcagaaag
      361 ttggagttct ctccacagac actgtgttgc tacggcaaac agttgtgcac aatacctcgt
      421 gatgccactt attacagtta ccagaacagg tatcatttct gtgagaagtg tttcaatgag
      481 atccaagggg agagcgtttc tttgggggat gacccttccc agcctcaaac tacaataaat
      541 aaagaacaat tttccaagag aaaaaatgac acactggatc ctgaactgtt tgttgaatgt
      601 acagagtgcg gaagaaagat gcatcagatc tgtgtccttc accatgagat catctggcct
      661 gctggattcg tctgtgatgg ctgtttaaag aaaagtgcac gaactaggaa agaaaataag
      721 ttttctgcta aaaggttgcc atctaccaga cttggcacct ttctagagaa tcgtgtgaat
      781 gactttctga ggcgacagaa tcaccctgag tcaggagagg tcactgttag agtagttcat
      841 gcttctgaca aaaccgtgga agtaaaacca ggcatgaaag caaggtttgt ggacagtgga
      901 gagatggcag aatcctttcc ataccgaacc aaagccctct ttgcctttga agaaattgat
      961 ggtgttgacc tgtgcttctt tggcatgcat gttcaagagt atggctctga ctgccctcca
     1021 cccaaccaga ggagagtata catatcttac ctcgatagtg ttcatttctt ccgtcctaaa
     1081 tgcttgagga ctgcagtcta tcatgaaatc ctaattggat atttagaata tgtcaagaaa
     1141 ttaggttaca caacagggca tatttgggca tgtccaccaa gtgagggaga tgattatatc
     1201 ttccattgcc atcctcctga ccagaagata cccaagccca agcgactgca ggaatggtac
     1261 aaaaaaatgc ttgacaaggc tgtatcagag cgtattgtcc atgactacaa ggatattttt
     1321 aaacaagcta ctgaagatag attaacaagt gcaaaggaat tgccttattt cgagggtgat
     1381 ttctggccca atgttctgga agaaagcatt aaggaactgg aacaggagga agaagagaga
     1441 aaacgagagg aaaacaccag caatgaaagc acagatgtga ccaagggaga cagcaaaaat
     1501 gctaaaaaga agaataataa gaaaaccagc aaaaataaga gcagcctgag taggggcaac
     1561 aagaagaaac ccgggatgcc caatgtatct aacgacctct cacagaaact atatgccacc
     1621 atggagaagc ataaagaggt cttctttgtg atccgcctca ttgctggccc tgctgccaac
     1681 tccctgcctc ccattgttga tcctgatcct ctcatcccct gcgatctgat ggatggtcgg
     1741 gatgcgtttc tcacgctggc aagggacaag cacctggagt tctcttcact ccgaagagcc
     1801 cagtggtcca ccatgtgcat gctggtggag ctgcacacgc agagccagga c
//
