LOCUS       sequence-241886-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B 
            Flag-Cas6 driven by hUbC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, 
            Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, 
            Beisel CL, Barrangou R, Gersbach CA
  TITLE     Targeted transcriptional modulation with type I CRISPR-Cas systems 
            in human cells.
  JOURNAL   Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7.
  PUBMED    31548729
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7."
COMMENT     Sequence Label: pLenti-LmoCas6
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             34..54
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggactaca aggacgacga cgataagatg gcgccaaaga aaaagcgcaa ggtccgcgga
       61 atgcggctga agattaactg cgacttcgac agcaagatca tccccaagga cttccagtcc
      121 aaggtcgtgt ccctgttcaa agccggaatt atgaaaagct ccccggagag atacgagaac
      181 ctgtttggtg gaaacaagca caagcagtac actttctcgg tgtatctccc aaaacctcag
      241 aacaagggcg ccgaaattca gctgaacgag gcgaactgta tcatcaactt ctccaccggc
      301 gatgctgaaa ccggtattat cttctacaac gccctgatta gcctgagagg ctccaaggtc
      361 ctgtttgggg ccggaaatca catcaccgtg aagaacatcc agatcgtgcc tgagaagaag
      421 atcatcggga agcaaaccat tctgcggacc ctttcgccgg tggtgtcgag ggatcacaac
      481 cgggaaacct tcaagaattg gttctactca ttcgaggacg aagaattcga gcctactttg
      541 aagcggaaca tgctgccgta cctcatcgac gcctttggag aacaggctcg ctacgatctg
      601 gaaaagctga agatcacgcc catttccatg aagaaagtcg tggtgtactg ccatgagatc
      661 cacatcgaga gctccgtggg catcttcgag ctggaagccg aggcatacct ccaaaagtac
      721 ctcgtggaaa acggaatcgg cactatgact ggctcagggt tcggaatgat tgaacagttc
      781 tga
//
