LOCUS       sequence-241914-       11024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of VLDLR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11024)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-VLDLR
FEATURES             Location/Qualifiers
     source          1..11024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5984..6008)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6016..6034
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6059..6078)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6060..6078)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6084..6104)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6158..6199
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6220..6787
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6237..6789
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6381..6398)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6608..6627
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6801..7400
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6801..6820)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7297..7317
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7414..8002
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7467..7487)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7885..7896)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8077..8310
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8429..8447)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8515..8619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8620..8688,8689..9480)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8838..8857)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9651..10239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10140..10159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10393..10410
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10650..10671
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10686..10691,10692..10709,10710..10716)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10724..10740
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10729..10751
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10748..10764
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10748..10764
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10814..10833
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcaccatg ggcacgtccg cgctctgggc gctctggctg
     3481 ctgctcgcgc tgtgctgggc gccccgggag agcggcgcca ccggaaccgg gagaaaagcc
     3541 aaatgtgaac cctcccaatt ccagtgcaca aatggtcgct gtattacgct gttgtggaaa
     3601 tgtgatgggg atgaagactg tgttgacggc agtgatgaaa agaactgtgt aaagaagacg
     3661 tgtgctgaat ctgacttcgt gtgcaacaat ggccagtgtg ttcccagccg atggaagtgt
     3721 gatggagatc ctgactgcga agatggttca gatgaaagcc cagaacagtg ccatatgaga
     3781 acatgccgca tacatgaaat cagctgtggc gcccattcta ctcagtgtat cccagtgtcc
     3841 tggagatgtg atggtgaaaa tgattgtgac agtggagaag atgaagaaaa ctgtggcaat
     3901 ataacatgta gtcccgacga gttcacctgc tccagtggcc gctgcatctc caggaacttt
     3961 gtatgcaatg gccaggatga ctgcagcgat ggcagtgatg agctggactg tgccccgcca
     4021 acctgtggcg cccatgagtt ccagtgcagc acctcctcct gcatccccat cagctgggta
     4081 tgcgacgatg atgcagactg ctccgaccaa tctgatgagt ccctggagca gtgtggccgt
     4141 cagccagtca tacacaccaa gtgtccagcc agcgaaatcc agtgcggctc tggcgagtgc
     4201 atccataaga agtggcgatg tgatggggac cctgactgca aggatggcag tgatgaggtc
     4261 aactgtccct ctcgaacttg ccgacctgac caatttgaat gtgaggatgg cagctgcatc
     4321 catggcagca ggcagtgtaa tggtatccga gactgtgtcg atggttctga tgaagtcaac
     4381 tgcaaaaatg tcaatcagtg cttgggccct ggaaaattca agtgcagaag tggagaatgc
     4441 atagatatca gcaaagtatg taaccaggag caggactgca gggactggag tgatgagccc
     4501 ctgaaagagt gtcatataaa cgaatgcttg gtaaataatg gtggatgttc tcatatctgc
     4561 aaagacctag ttataggcta cgagtgtgac tgtgcagctg ggtttgaact gatagatagg
     4621 aaaacctgtg gagatattga tgaatgccaa aatccaggaa tctgcagtca aatttgtatc
     4681 aacttaaaag gcggttacaa gtgtgaatgt agtcgtggct atcaaatgga tcttgctact
     4741 ggcgtgtgca aggcagtagg caaagagcca agtctgatct tcactaatcg aagagacatc
     4801 aggaagattg gcttagagag gaaagaatat atccaactag ttgaacagct aagaaacact
     4861 gtggctctcg atgctgacat tgctgcccag aaactattct gggccgatct aagccaaaag
     4921 gctatcttca gtgcctcaat tgatgacaag gttggtagac atgttaaaat gatcgacaat
     4981 gtctataatc ctgcagccat tgctgttgat tgggtgtaca agaccatcta ctggactgat
     5041 gcggcttcta agactatttc agtagctacc ctagatggaa ccaagaggaa gttcctgttt
     5101 aactctgact tgcgagagcc tgcctccata gctgtggacc cactgtctgg ctttgtttac
     5161 tggtcagact ggggtgaacc agctaaaata gaaaaagcag gaatgaatgg attcgataga
     5221 cgtccactgg tgacagcgga tatccagtgg cctaacggaa ttacacttga ccttataaaa
     5281 agtcgcctct attggcttga ttctaagttg cacatgttat ccagcgtgga cttgaatggc
     5341 caagatcgta ggatagtact aaagtctctg gagttcctag ctcatcctct tgcactaaca
     5401 atatttgagg atcgtgtcta ctggatagat ggggaaaatg aagcagtcta tggtgccaat
     5461 aaattcactg gatcagagct agccactcta gtcaacaacc tgaatgatgc ccaagacatc
     5521 attgtctatc atgaacttgt acagccatca ggtaaaaatt ggtgtgaaga agacatggag
     5581 aatggaggat gtgaatacct atgcctgcca gcaccacaga ttaatgatca ctctccaaaa
     5641 tatacctgtt cctgtcccag tgggtacaat gtagaggaaa atggccgaga ctgtcaaagg
     5701 atcaatgtga ccacagcagt atcagaggtc agtgttcccc caaaagggac ttctgccgca
     5761 tgggccattc ttcctctctt gctcttagtg atggcagcag taggtggcta cttgatgtgg
     5821 cggaattggc aacacaagaa catgaaaagc atgaactttg acaatcctgt gtacttgaaa
     5881 accactgaag aggacctctc catagacatt ggtagacaca gtgcttctgt tggacacacg
     5941 tacccagcaa tatcagttgt aagcacagat gatgatctag ctttgccaac tttcttgtac
     6001 aaagtggtga tatccaatta accctcacta aaggatgatg tgtttgattt gaattgaacc
     6061 ctatagtgag tcgtattacc acccaacttt tctatacaaa gtggttgata tccagcacag
     6121 tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
     6181 cctcggtctc gattctacgc gtaccggtta gtaatgagat ccctcccccc cccctaacgt
     6241 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     6301 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     6361 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     6421 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     6481 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     6541 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     6601 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     6661 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     6721 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     6781 atgataatat ggccacacat atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     6841 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     6901 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     6961 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     7021 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     7081 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     7141 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     7201 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     7261 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     7321 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     7381 cccgcaagcc cggtgcctaa atcgatagat cctaatcaac ctctggatta caaaatttgt
     7441 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7501 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7561 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7621 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7681 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7741 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7801 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     7861 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7921 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7981 tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg
     8041 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     8101 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     8161 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8221 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8281 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     8341 atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa
     8401 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     8461 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     8521 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8581 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8641 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8701 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8761 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8821 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     8881 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8941 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     9001 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     9061 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     9121 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9181 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9241 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9301 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9361 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9421 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9481 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9541 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9601 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9661 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9721 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9781 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     9841 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9901 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9961 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    10021 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    10081 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    10141 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    10201 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    10261 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    10321 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    10381 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    10441 cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt
    10501 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    10561 gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac
    10621 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
    10681 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
    10741 tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca
    10801 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    10861 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    10921 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    10981 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
