LOCUS       sequence-241959-       10038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Localized Pan-Neuronal GCaMP6s.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Sharma AK, Randi F, Kumar S, Dvali S, Leifer AM
  TITLE     TWISP: a transgenic worm for interrogating signal propagation in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Jul 8;227(3):iyae077. doi: 10.1093/genetics/iyae077.
  PUBMED    38733622
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-07-8"; volume: "227(3):iyae077. doi"; pages: " 
            10.1093/genetics/iyae077"
COMMENT     Sequence Label: pAS1-rab-3P::his-24::GCaMP6s
FEATURES             Location/Qualifiers
     source          1..10038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     83..105
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     97..114
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     98..114
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     124..143
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        124..142
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(5230..5238,5239..5256,5257..5259,5260..5292,5293..5295,
                     5296..5319,5320..5346,5347..5403,5404..5409,5410..5679,
                     5680..5697,5698..6132,6133..6138,6139..6582)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      5326..5335
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     5616..5637
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5743..5764)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6004..6023)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(7348..7368)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7348..7366)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7387..7403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7387..7403)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7400..7422)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7411..7427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7435..7441,7442..7459,7460..7465))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7480..7501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7618..7635)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7789..8377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7869..7888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8548..9339,9340..9408))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9171..9190
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9409..9513)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9539..9994)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9676..9697)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9888..9907
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
       61 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
      121 gcgtaatacg actcactata gggcgaattg gagctccacc gcggactagt ggatcccccg
      181 ggctgcaggc gagttttgac tggctttcaa aaactgagtg attactaatt atttttgaaa
      241 aaattaacag ttgtgatatg atatttagac atttttgcaa tatataattt gtttgaaaaa
      301 aaaacatttt ttaatggtct tcgaagctat aatagttttt cgtaaaattt taaataaaac
      361 agctgacaga tatccacatt caataattga aagtatgcag ttctcttttt ttttgtcaaa
      421 tagaaaaaaa gtaacagatg gtcagatata atctaggtta atctgactct ggtcatcgat
      481 gaactcattt tcatcataat cattcttctt tcaaatggtc tgttcggaat tcgaatattc
      541 aataaatgga tatcttatta gatgctccgg atttattcca ccaaacttcc aagacgtaag
      601 ttcaatttaa acaaaattac tatagcttga aaaaatgaga aaaaacgagc agataaaaaa
      661 ataacagaaa aaatccataa aaatgacgag ggttccgtgc gaaaatcgtc attgtttttg
      721 gcttgcatca cacttttccg cgagtcagcc acactgtttt atcgattatt atttttctga
      781 tttcagaaac cggaaactta caactgagat gctttcattt tgattttttt aaatttagtt
      841 ttgatttcag cgatttgttt gaatataatt atgcatgagt tggcttttgc atttttagat
      901 aaataagttc acatgctcgg acaaattgtg tggtttaatc tgtgaaagtt ttgaatcttc
      961 tgtttgaaaa aaaaaacatt tttaatttgt atatacgagt gaattggtta ggccctggtt
     1021 acgttttcca gcgaattcat tcaaatttct tattttttct attttttcaa ttaaataatt
     1081 tagttctcta tcacatgtgt atgatcaaaa tttcattcct aatcgtaagc aaatttaggc
     1141 tcatccgtag tataattttg ttatgaaggg cttcacagta aaagctcaaa ctccaacatt
     1201 tagcaactag caaccaacaa aaaaccaaag aaatgtcagt tttgaaaagt cgttatttct
     1261 ttgtcttctc acaattcaca catcatcttc tcgttttcct ccgttttttt gtcgtgcttc
     1321 cttcttttct ttacacattt ttctaacctt tttttaagtc tatcctctcc taatcctttg
     1381 tactctctaa tcagttttgc tggttgtgct gcacaatttt tatcagattt atgcgcgaaa
     1441 caaaagcctc tcgcggagag ctccgccaaa tccggtcttc ttttttcctt gcctatcgga
     1501 tttcacaaac attcagtttg tcacttgacg atgtgttgga ggcattacta ctaggaagtg
     1561 ttattcatat tttttattcg tttctttttg gtgactaaac gaaatattcc gaacgttttc
     1621 ctgatttcag gcggcggaaa cgaagaagac cacgaataat caacaggctg ccattgcatc
     1681 cgccagaagt cgagtgagtt ctttgaaaat ctttattaga gttatttgtg acaatttgcc
     1741 actttttttt taatgtttaa tgaatctgta acgaaattaa taccaagggt gtccaacaga
     1801 actatctgag cggttattta atttttagat agtatatttc ttccgatcgt aacatacata
     1861 ttttcaagca tagtactgtg atttaagtta cagttttcca atcagtcttg attttaataa
     1921 aaacttttca actgcaaaaa tgcctctgaa gtatttttct atatttttat agttgaataa
     1981 ttttttgatt ggcacaaaga ccgccgagat atgagccgtt aaagtaaagc tgcatggatt
     2041 caatatggtt tgtatttgat tggagtcact tacatggaaa tatttgtatt taaatctcat
     2101 attttaaaac aagacaaaca ccaaattaaa tttttattaa tctgaacatt tttcaatcga
     2161 tttggtaaac aaattattag atgtttccct caaaaattgt aacaattcct aaattgtggc
     2221 acttataata acatcgtcct gaattctcaa aaaaaaccga atgctaagag agatttcgtt
     2281 taactgggac ctgtaactga attttcaaat attggcagtt tttgataaac tgggagggaa
     2341 atcaaaaacc aaaactacag tacccctcgt gttataataa attgtcattc actccttttt
     2401 tgttcccaac atttgctccc atctcctgac gagaaagaac aattttatga gcacgggaac
     2461 ccgtgggcgt ccatccgttc ctcctgtgca atttcagccc ccttcctcta cattctctgt
     2521 gttcccgttt ttatcgtttt atgggtatcg ggaacggaca gatcgcttcc atttgcttct
     2581 attccgtcct cacaagacgt cccactaact gattttcttc tgatttcttt tcactgaaaa
     2641 tgggaaagaa agaaaaagac gatgaatcga cgacttcttc tcaggagaag gtgaagaaga
     2701 aggataagga gaaagttcat ctaaggctga aagataaagt aaaaaaactt agaaaatcta
     2761 agtcgattgc agtatcttct ccagctgatt atatgacttg tcagtgtgaa ccatgcaaaa
     2821 aagtagctct cgaggtaata atttttaaac tcaatacgta gttaagtttg aactcctttt
     2881 aatttactta tcatctaatt taccgtcaca atagtacttc cattagcaat tattttaaaa
     2941 ttacatgcat agcttcaatg ttcgtttttt tgcgttctca tatcgtatgt tgtctgcctc
     3001 tcgaatactg actgtccgac tagaatgtcc cggtaggatt actgtacggt cactgggaat
     3061 aaactgtttc tgaaatcaat ctgaaattcc gtagatcttc ttggttcaat ttcttattcc
     3121 tcaatctttg gacaacgtca ccttcagagt cgtcgttgag tatcccatca ttaactagtc
     3181 aatctttctc tctttttcat ttcccccaac ttttctgcat attttttgac gacgacgacc
     3241 tcgacggcaa cgagaataca tttatctatt ttttagtata gcatatatac attttctact
     3301 tttctctaac ccatttcaga cgtatagatt ttggttttcg aatgcaactt tgagattttt
     3361 caaagatctt cagatgggag cagtggactg tctatttgat attcttaaaa tgtactctat
     3421 aaaagatgaa actgtaatga ccaattccct acataaatat ttttaactaa ttggtcggat
     3481 atttggggat cagagaagta cttgtttttg cgatattaat aatgatatat ttttctagta
     3541 aaaaaatgtt aagcgatttg tgaataatta agttggcttg aaaaagattt tttgatagct
     3601 atgtattttg ttaatttaaa tcagatttta aaactttatt attgactatt tatatatatt
     3661 atatattata tattttcatg gtttaatcaa aatctattaa attatttcta agatctctaa
     3721 atatcgaatt ctctaaaaag cttcatcaac aaaagcattt caattatcta acacaatgag
     3781 aataattcaa gaactgaaat agaaagaaat ctagaaaaaa aagagagtga ggtgccctat
     3841 taaatcggtg ctctcctctc taacagaagc gcctacacgc acccaagaga gctctttttc
     3901 tttctttttt cggagcacac atacacgcat acactcctca gaagtgtata gagaaaatgg
     3961 gagaagaagc gaaagaagac gaagaagcgg ttgtgatgta tgcatcgggc gtttctatcc
     4021 ctctccgtga gcaacgagct agtcaaccca tcgatccatt tttgtgagca cacacagaga
     4081 gagactcaaa cacactcaca gcttcacact gagctccaag agctctgtag agagaagcca
     4141 gagtatgctt tcctcgattt tcctaacgtc ttcttcttct gcttttttct ttttcccaac
     4201 gagttgtagt ccactaactt caatttttcc tccagagaaa gagttttaga gagacagctg
     4261 ataaatacac tagagaaata aaaagaatga taaaaatttg ctattttaat cagttccctc
     4321 tcgtttctct aagttttgtt gttgggttgg gaaattaaaa ttggaaaatg gaaataacgt
     4381 ttttttctgt ctaatcttga attctccaga catttcttat ttcctttttt gttgcaagtt
     4441 tttcttcctt ctctaatttt taatcaaaga tatctcgcta tgaactttga atttttgata
     4501 ttttgattct ataaatacat aaagtaatct aaatgctctt ttaaaataaa tctacagtag
     4561 ccctattttc agcctaggat gtctgattcc gctgttgttg ccgccgctgt cgagccaaag
     4621 gtcccaaagg ctaaggccgc caaggccgcc aagccagccg ccaaggccgc caagccaacc
     4681 aaagttgcca aggccaaggc tccagtcgct cacccaccat acatcaacat gatcaaggag
     4741 gccatcaagc agctcaagga ccgcaaagga gcttccaagc aggcaatcct caagttcatc
     4801 tcccaaaact acaagctcgg agacaatgtc atccagatca atgctcatct ccgtcaggct
     4861 ctcaagcgtg gtgtcaccag caaggccctt gttcaagccg ccggatccgg agccaacgga
     4921 cgtttccgtg tgccagagaa ggccgccgcc gccaagaagc cagcagcagc caagaagcca
     4981 gcagcagcca agaaaccagc agccgccaag aaagccactg gagagaagaa ggcaaagaag
     5041 ccagccgctg ccaagccaaa gaaggccgcc accggagata agaaggtcaa gaaggccaag
     5101 agcccaaaga aggttgccaa gccagctgcc aagaaggtcg ccaagtctcc agccaagaag
     5161 gccgctccaa agaagatcgc caagccagct gccaagaagg ccgccaagcc agccgccaag
     5221 gccgcatgca tgggttctca tcatcatcat catcatggta tggctagcat gactggtgga
     5281 cagcaaatgg gtcgggatct gtacgacgat gacgataagg atctcgccac catggtcgac
     5341 tcatcacgtc gtaagtggaa taagacaggt cacgcagtca gagctatagg tcggctgagc
     5401 tcactcgaga acgtctatat caaggccgac aagcagaaga acggcatcaa ggcgaacttc
     5461 cacatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta ccagcagaac
     5521 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cgtgcagtcc
     5581 aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     5641 gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg
     5701 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     5761 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc cacctacggc
     5821 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     5881 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     5941 cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac catcttcttc
     6001 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     6061 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     6121 ctggagtaca acctgccgga ccaactgact gaagagcaga tcgcagaatt taaagaggct
     6181 ttctccctat ttgacaagga cggggatggg acaataacaa ccaaggagct ggggacggtg
     6241 atgcggtctc tggggcagaa ccccacagaa gcagagctgc aggacatgat caatgaagta
     6301 gatgccgacg gtgacggcac aatcgacttc cctgagttcc tgacaatgat ggcaagaaaa
     6361 atgaaataca gggacacgga agaagaaatt agagaagcgt tcggtgtgtt tgataaggat
     6421 ggcaatggct acatcagtgc agcagagctt cgccacgtga tgacaaacct tggagagaag
     6481 ttaacagatg aagaggttga tgaaatgatc agggaagcag acatcgatgg ggatggtcag
     6541 gtaaactacg aagagtttgt acaaatgatg acagcgaagt gaaagctttg gaattcatct
     6601 cgcgcccgtg cctctgactt ctaagtccaa ttactcttca acatccctac atgctctttc
     6661 tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt
     6721 tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt
     6781 aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat
     6841 cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt
     6901 tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt
     6961 tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc
     7021 atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat
     7081 taattttcct gcttttgctt tttggggttt cccctattgt ttgtcaagat ttcgaggacg
     7141 gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa
     7201 ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag
     7261 tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca aacataactg
     7321 tttgggcccg gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     7381 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7441 acgagccggg agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7501 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7561 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7621 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7681 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7741 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7801 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7861 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7921 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7981 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8041 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8101 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8161 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8221 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8281 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8341 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8401 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8461 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8521 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8581 agcgatctgc ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8641 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8701 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8761 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8821 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8881 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8941 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9001 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9061 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9121 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9181 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9241 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9301 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9361 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9421 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9481 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
     9541 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     9601 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     9661 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     9721 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     9781 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     9841 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     9901 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     9961 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
    10021 tgttgggaag ggcgatcg
//
