LOCUS       sequence-241987-        3847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for sense in situ probe in vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Castro JP, Yancoskie MN, Marchini M, Belohlavy S, Hiramatsu L, Kucka
            M, Beluch WH, Naumann R, Skuplik I, Cobb J, Barton NH, Rolian C, 
            Chan YF
  TITLE     An integrative genomic analysis of the Longshanks selection 
            experiment for longer limbs in mice.
  JOURNAL   Elife. 2019 Jun 6;8:e42014. doi: 10.7554/eLife.42014.
  PUBMED    31169497
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-06-6"; volume: "8:e42014. doi"; pages: " 
            10.7554/eLife.42014"
COMMENT     Sequence Label: pJET1.2_Bod1l_Sense
FEATURES             Location/Qualifiers
     source          1..3847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    88..109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(124..129,130..147,148..154)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(1509..1528)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1510..1528)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1834..1852)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1920..2024
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2025..2093,2094..2885)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2243..2262)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3056..3644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3545..3564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3798..3815
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
       61 agcaattggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      121 ggctttacac tttatgcttc cggctcgtat aatgtgtgga attatgagcg gataataatt
      181 tcacacagga ggtttaaact ttaaacatgt caaaagagac gtcttttgtt aagaatgctg
      241 aggaacttgc aaagcaaaaa atggatgcta ttaaccctga actttcttca aaatttaaat
      301 ttttaataaa attcctgtct cagtttcctg aagcttgctc taaacctcgt tcaaaaaaaa
      361 tgcagaataa agttggtcaa gaggaacata ttgaatattt agctcgtagt tttcatgaga
      421 gtcgattgcc aagaaaaccc acgccaccta caacggttcc tgatgaggtg gttagcatag
      481 ttcttaatat aagttttaat atacagcctg aaaatcttga gagaataaaa gaagaacatc
      541 gattttccat ggcagctgag aatattgtag gagatcttct agaaagatca ctgtgagttc
      601 gtcatcagaa tcactgtagt actttgagta aagatacggt ttgtgaacat aagcatgccg
      661 tacgcctctg gcttttccat cactagaatc accaggttga acttttgctt tattctgctt
      721 gtttttctct tcatcctctg atgtaatctc tccttcttcc atgctctcag acacgacaac
      781 ttcctcctct gtgtcttctt caaaggacga aaggtcactg gtgtggacag agctgactgt
      841 gatatcagtg agaccatcca catcagaatc taacaaagaa caatcttctt ttgtcccctc
      901 agcagccctg gctctgctgt tgctcttttc agaaggaagc tttgtgctct ccacttcttt
      961 gtcctttgct tgtctctcat ctttcacttt ctgtgtatct tcattccttt tagagtgatc
     1021 aatttttttt tcagtctttt ccttcttctc tttctttctc tctcctttct cattaccatc
     1081 gggtttcttt tctcctttgt ccattgattt aatttttggg tcagtgcttt cctgctgagc
     1141 atccttactt agtaacagta agctattatt ctctttcata tgatttttag tttcttccac
     1201 tggacaaggg agatcactgg gttcttctga cttgagagca gcttctagcc catctcctcc
     1261 agaatcagag gtagcctttt ctctctcagt cgcatcttct gagcctctct ccttgtctgt
     1321 gctaacgtca gtgcttggct gagatgagag ttttctggat gttctctcac tgaccttggc
     1381 atttgacatt tctgttgaag ctctggcagc gttagcttct tggttaagag aagttatggt
     1441 ttccaagatt gacatggcat catcttgctg aaaaactcga gccatccgga agatctggcg
     1501 gccgctctcc ctatagtgag tcgtattacg ccggatggat atggtgttca ggcacaagtg
     1561 ttaaagcagt tgattttatt cactatgatg aaaaaaacaa tgaatggaac ctgctccaag
     1621 ttaaaaatag agataatacc gaaaactcat cgagtagtaa gattagagat aatacaacaa
     1681 taaaaaaatg gtttagaact tactcacagc gtgatgctac taattgggac aattttccag
     1741 atgaagtatc atctaagaat ttaaatgaag aagacttcag agcttttgtt aaaaattatt
     1801 tggcaaaaat aatataattc ggctgcaggg gcggcctcgt gatacgccta tttttatagg
     1861 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     1921 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1981 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2041 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2101 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2161 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2221 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     2281 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     2341 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2401 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2461 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2521 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     2581 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2641 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2701 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2761 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2821 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2881 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2941 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3001 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3061 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3721 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3781 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3841 aaccgcc
//
