LOCUS       sequence-242004-       11199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre/lox flip-out construct for marking cell clones in Tribolium 
            castaneum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11199)
  AUTHORS   Gilles AF, Schinko JB, Schacht MI, Enjolras C, Averof M
  TITLE     Clonal analysis by tunable CRISPR-mediated excision.
  JOURNAL   Development. 2019 Jan 4;146(1). pii: dev.170969. doi: 
            10.1242/dev.170969.
  PUBMED    30552128
REFERENCE   2  (bases 1 to 11199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2019-01-4"; volume: "146(1). pii: dev.170969.
            doi"; pages: " 10.1242/dev.170969"
COMMENT     Sequence Label: pBac(3xP3-gTc'v; pUb:lox-mYFP-lox-H2BmCherry)
FEATURES             Location/Qualifiers
     source          1..11199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    88..109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(124..129,130..147,148..154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    162..178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     167..189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     186..202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     186..202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   383..417
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    complement(2766..2799)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             join(2872..2874,2875..2877,2878..3591)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2917..2938)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3178..3197)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3525..3546
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3714..3835
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3842..3875)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             4259..4966
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4404..4422)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4672..4691
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4976..5032
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    5102..5223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   8019..8081
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8785..8802)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8785..8801)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8794..8816)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9010..9029)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9129..9151
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9189..9207)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9275..9379
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9380..9448,9449..10240)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9598..9617)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10411..10999
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10900..10919
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11153..11170
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
       61 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      121 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      181 tcacacagga aacagctatg acatgattac gaattcgagc tcggtacccg gggatcctct
      241 agagtcgacg ctcgcgcgac ttggtttgcc attctttagc gcgcgtcgcg tcacacagct
      301 tggccacaat gtggtttttg tcaaacgaag attctatgac gtgtttaaag tttaggtcga
      361 gtaaagcgca aatctttttt aaccctagaa agatagtctg cgtaaaattg acgcatgcat
      421 tcttgaaata ttgctctctc tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca
      481 tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga
      541 ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg attatctttt acgtgacttt
      601 taagatttaa ctcatacgat aattatattg ttatttcatg ttctacttac gtgataactt
      661 attatatata tattttcttg ttatagatat cgtgactaat atataataaa atgggtagtt
      721 ctttagacga tgagcatatc ctctctgctc ttctgcaaag cgatgacgag cttgttggtg
      781 aggattctga cagtgaaata tcagatcacg taagtgaaga tgacgtccag agcgatacag
      841 aagaagcgtt tatagatgag gtacatgaag tgcagccaac gtcaagcggt agtgaaatat
      901 tagacgaaca aaatgttatt gaacaaccag gttcttcatt ggcttctaac agaatcttga
      961 ccttgccaca gaggactatt agaggtaaga ataaacattg ttggtcaact tcaaagtcca
     1021 cgaggcgtag ccgagtctct gcactgaaca ttgtcagatc tcaaagaggt ccgacgcgta
     1081 tgtgccgcaa tatatatgac ccacttttat gcttcaaact attttttact gatgagataa
     1141 tttcggaaat tgtaaaatgg acaaatgctg agatatcatt gaaacgtcgg gaatctatga
     1201 caggtgctac atttcgtgac acgaatgaag atgaaatcta tgctttcttt ggtattctgg
     1261 taatgacagc agtgagaaaa gataaccaca tgtccacaga tgacctcttt gatcgatctt
     1321 tgtcaatggt gtacgtctct gtaatgagtc gtgatcgttt tgattttttg atacgatgtc
     1381 ttagaatgga tgacaaaagt atacggccca cacttcgaga aaacgatgta tttactcctg
     1441 ttagaaaaat atgggatctc tttatccatc agtgcataca aaattacact ccaggggctc
     1501 atttgaccat agatgaacag ttacttggtt ttagaggacg gtgtccgttt aggatgtata
     1561 tcccaaacaa gccaagtaag tatggaataa aaatcctcat gatgtgtgac agtggtacga
     1621 agtatatgat aaatggaatg ccttatttgg gaagaggaac acagaccaac ggagtaccac
     1681 tcggtgaata ctacgtgaag gagttatcaa agcctgtgca cggtagttgt cgtaatatta
     1741 cgtgtgacaa ttggttcacc tcaatccctt tggcaaaaaa cttactacaa gaaccgtata
     1801 agttcgagat cggccggcct tttctttgtc ccaaatgacc cttttacata atttattatt
     1861 gtcgtccgta tttacatgat aaacaagact ctagaacaac aacgataagg ataattcttt
     1921 gtaaacaaat taattcgttt caaaattgaa cagacgaata tggcattcca tgcaggaatt
     1981 aatgggtggt ttatttgaaa cgtttgaaaa gcataaattg ggaaataaat taaaatttga
     2041 aaaataaaaa agggcgtaat tccaaaacaa agaaaacagc tgatcgataa agctgacgac
     2101 gtagataaaa atcagctgac atgtcaccaa caactgctgg ccaaagtccc taaaatcttg
     2161 ttcaatcaaa agcaattttc ttaatacctg aaacattgtt tagaccatga ttaaccgaat
     2221 tggcattaaa ctaacagcgg ctaacgtttg tgcataaatt tttgattaaa gaggacgttt
     2281 tttggataat tactcgaaaa ttctagaaag tgtactttgg gttcgagatt aaaaaggagt
     2341 gggggaagcc gtccgtataa aagcggttcc acagctgcat tcgccatatt gtctgagcaa
     2401 aagtggtgaa atacaaaagc gatttaatta aaattgtgaa ttttcctagc cgtaaaggta
     2461 tgtattttaa tttcatcatg aattattcga ttgcaagaag cgcagaaagt cgcaattttg
     2521 tgaatttttt cgcatgacgc aataacattt gtttgaaaaa acatcgccct agtgacgtgt
     2581 tttttgttta aattttaaca aaattaattt tgtaaatttg tcgatctgtt caaaaccaaa
     2641 ccacatagtg agtaatgtgt tttttcggtt tattcagtgc gtaattcttt gttcaacgaa
     2701 atccaatatg gcggcctgga aatttgcttt gtcacaaaag taattttttg tgtcgttgca
     2761 gatctataac ttcgtatagt ataccttata cgaagttatt gcgatcgcaa aatgctgtgc
     2821 tgtatgagaa gaaccaaaca ggttgaaaag aatgatgagg accaaaagat catggtgagc
     2881 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2941 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     3001 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     3061 accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     3121 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     3181 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     3241 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     3301 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     3361 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     3421 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     3481 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3541 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc
     3601 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     3661 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     3721 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3781 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct
     3841 tataacttcg tatagtatac cttatacgaa gttattacgt aaaatggctc cgaaaactag
     3901 tggaaaggca gccaagaagg ctggcaaggc tcagaagaac atcaccaaga ccgacaagaa
     3961 aaagaagcgc aaaaggaagg agagctatgc catctacatt tacaaggttc tcaagcaggt
     4021 ccatcctgac accggaattt cgtcgaaggc gatgagcata atgaacagct ttgtaaatga
     4081 tattttcgag cgaattgctg ccgaagcgtc tcgtctagct cactacaaca agcgctcgac
     4141 catcaccagt cgggagatcc aaacggctgt tcgcctgctt ttgcctggag agttggccaa
     4201 gcatgctgtc agtgagggaa ccaaggctgt caccaagtac accagctcta aactcgagat
     4261 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     4321 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     4381 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     4441 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     4501 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     4561 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaagacgg
     4621 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     4681 gcagaagaag acgatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     4741 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     4801 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     4861 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     4921 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggat ccggagccac
     4981 gaacttctct ctgttaaagc aggcaggaga tgtggaagaa aaccccggcc ctgccatggt
     5041 taattaacct gcaggctaga tcataaactc atcggcgcgc cgtacgcgta tcgataagct
     5101 ttaagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     5161 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     5221 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     5281 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct agagtcgcgg
     5341 ccgccttaac gaaataatta attgtttcca ttcgacacgt tattggtgct attcgccgac
     5401 ccccaattgc acaaatgact tctcatagac gtagacaagg gggggatgta ggaccgtggt
     5461 atcaaaaacg tcgataaatt gaaaaggtcc acgaaaactt tataccgatc gctgaaaaac
     5521 aaaatcaaac attgcaaaaa ccgtgttgaa atcaatttca ccttaacgtt gaccttaaat
     5581 actgataccc cgaagagccc cctgttccta actgcgaaga accaatcatt ctttgcacca
     5641 tcaaaacatg attatctgaa attataaaat ttaaacaaaa acaattgacg tacggattta
     5701 ctaacaccgc catttcgtaa tcagcgagtc tatgtccatc aaaagtgtta gaatttggtg
     5761 gggctgactg aacctgggtt cgtccctgta aaaggtgatc atgatagccc cttgcagggc
     5821 tttgtgtgag aagcgcctct cgccgcgcga cattagagcc tcatggacct caactttaaa
     5881 aatcgactcg taaacctcgc gtctcttctc caaatcgttc aatttgtaac gctttaaagt
     5941 ctccgcctcc tctttctccg ccagttcttt ttgctcggtt aaaagcttct cgaccgcttt
     6001 ttggtacttc ccccagaaat taaacccctc gagctccagc cccggcgttc gctccagcca
     6061 ccgctggacc aaatcggtca aagacggctc tttctccgac ttggcgattt gctcgagcgc
     6121 tttttcgtcg tttccgaaca ctttcgagta attctggttg tatttgacgc ggttttcctg
     6181 gcgcaccccg agcttatttt ccaggagcct gaactgcagg ctctggaacc cggaagcggg
     6241 ccgcagataa caccggaagt ccatgaaatc gagcggtgtc atcgtttcca gaatcattac
     6301 ttggtccacc aaaacctaca attgcgacag ttttatctcg tgaccgcaat ttgttgcgaa
     6361 attgtgaacc ttgaggatca aaacaacacg attgaggcgt ttgaggattt ccaaagtttg
     6421 cgactcttct aaaacgtcgc tgaaaatgtt gcggatcgag tccagctcgt agattatttg
     6481 tttgaaccat aactcatacg ctggaattac ttgttatagt taattacggt taagtgggtt
     6541 ttttaccttg atgggtcaca ataaataagt gctcgtcgtg cacgggttgg ttattttgct
     6601 cgctgagtag tctttgggct tctaaaatct tgtccaacat caagtattca ccgtaaagca
     6661 tcccacattc ttcgctcagt tggtcgcctt cttgggcttc acttaattta cgattaattg
     6721 taaatggaat aattaaatta aatcatactt acgagggtct cagtgggcaa ctcattacga
     6781 ccgcggtacc gtcgactcta gcggtacccc gattgtttag cttgttcagc tgcgcttgtt
     6841 tatttgctta gctttcgctt agcgacgtgt tcactttgct tgtttgaatt gaattgtcgc
     6901 tccgtagacg aagcgcctct atttatactc cggcggtcga gggttcgaaa tcgataagct
     6961 tggatcctaa ttgaattagc tctaattgaa ttagtctcta attgaattag atccccgggc
     7021 gagctcgaat taaccattgt gggaaccgtg cgatcaaaca aacgcgagat accggaagta
     7081 ctgaaaaaca gtcgctccag gccagtggga acatcgatgt tttgttttga cggacccctt
     7141 actctcgtct catataaacc gaagccagct aagatggtat acttattatc atcttgtgat
     7201 gaggatgctt ctatcaacga aagtaccggt aaaccgcaaa tggttatgta ttataatcaa
     7261 actaaaggcg gagtggacac gctagaccaa atgtgttctg tgatgacctg cagtaggaag
     7321 acgaataggt ggcctatggc attattgtac ggaatgataa acattgcctg cataaattct
     7381 tttattatat acagccataa tgtcagtagc aagggagaaa aggttcaaag tcgcaaaaaa
     7441 tttatgagaa acctttacat gagcctgacg tcatcgttta tgcgtaagcg tttagaagct
     7501 cctactttga agagatattt gcgcgataat atctctaata ttttgccaaa tgaagtgcct
     7561 ggtacatcag atgacagtac tgaagagcca gtaatgaaaa aacgtactta ctgtacttac
     7621 tgcccctcta aaataaggcg aaaggcaaat gcatcgtgca aaaaatgcaa aaaagttatt
     7681 tgtcgagagc ataatattga tatgtgccaa agttgtttct gactgactaa taagtataat
     7741 ttgtttctat tatgtataag ttaagctaat tacttatttt ataatacaac atgactgttt
     7801 ttaaagtaca aaataagttt atttttgtaa aagagagaat gtttaaaagt tttgttactt
     7861 tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat aaataaattg
     7921 tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat atctattcaa
     7981 attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt ttacgcatga
     8041 ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaaataat agtttctaat
     8101 ttttttatta ttcagcctgc tgtcgtgaat accgtatatc tcaacgctgt ctgtgagatt
     8161 gtcgtattct agccttttta gtttttcgct catcgacttg atattgtccg acacattttc
     8221 gtcgatttgc gttttgatca aagacttgag cagagacacg ttaatcaact gttcaaattg
     8281 atccatatta acgatatcaa cccgatgcgt atatggtgcg taaaatatat tttttaaccc
     8341 tcttatactt tgcactctgc gttaatacgc gttcgtgtac agacgtaatc atgttttctt
     8401 ttttggataa aactcctact gagtttgacc tcatattaga ccctcacaag ttgcaaaacg
     8461 tggcattttt taccaatgaa gaatttaaag ttattttaaa aaatttcatc acagatttaa
     8521 agaagaacca aaaattaaat tatttcaaca gtttaatcga ccagttaatc aacgtgtaca
     8581 cagacgcgtc ggcaaaaaac acgcagcccg acgtgttggc taaaattatt aaatcaactt
     8641 gtgttatagt cacggatttg ccgtccaacg tgttcctcaa aaagttgaag accaacaagt
     8701 ttacggacac tattaattat ttgattttgc cccacttcat tttgtgggat cacaattttg
     8761 ttatattttt aaacaaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     8821 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     8881 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     8941 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     9001 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     9061 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     9121 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     9181 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     9241 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     9301 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     9361 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     9421 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     9481 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     9541 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     9601 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     9661 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     9721 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     9781 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     9841 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     9901 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     9961 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    10021 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    10081 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    10141 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    10201 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    10261 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    10321 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    10381 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    10441 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    10501 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    10561 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    10621 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    10681 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    10741 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    10801 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    10861 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    10921 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    10981 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    11041 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    11101 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    11161 agtgagcgag gaagcggaag agcgcccaat acgcaaacc
//
