LOCUS       sequence-242114-        8328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector (pRRL) containing KRAS G12V specific T cell 
            receptor, clone 9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Veatch JR, Jesernig BL, Kargl J, Fitzgibbon M, Lee SM, Baik C, 
            Martins R, Houghton AM, Riddell SR
  TITLE     Endogenous CD4(+) T Cells Recognize Neoantigens in Lung Cancer 
            Patients, Including Recurrent Oncogenic KRAS and ERBB2 (Her2) Driver
            Mutations.
  JOURNAL   Cancer Immunol Res. 2019 Jun;7(6):910-922. doi: 
            10.1158/2326-6066.CIR-18-0402. Epub 2019 May 1.
  PUBMED    31043415
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2326-6066.CIR-18-0402"; journalName: "Cancer Immunol Res"; 
            date: "2019-06"; volume: "7"; issue: "6"; pages: "910-922"
COMMENT     Sequence Label: pJV136
FEATURES             Location/Qualifiers
     source          1..8328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      386..395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1340..1396
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    2234..2822
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2287..2307)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2705..2716)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2909..3142
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3214..3335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3251..3270)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3305..3324
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3375..3510
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3530..3549)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3531..3549)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3559..3576)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3559..3575)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3568..3590)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3717..4172
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3804..3823)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4014..4035
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4198..4302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4303..4371,4372..5163)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4521..4540)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5334..5922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5823..5842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6076..6093
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6210..6231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6246..6251,6252..6269,6270..6276)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6284..6300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6289..6311
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6308..6324
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6308..6324
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6343..6363
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6345..6363
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6391..6617
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6618..6798
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6845..6970
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7463..7696
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7881..7925
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8192..8309
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcgagggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
       61 gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga
      121 gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
      181 gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat
      241 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
      301 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
      361 ccacaacccc tcactcggcg cgccggccac catgggatct tggacactgt gttgcgtgtc
      421 cctgtgcatc ctggtggcca agcacacaga tgccggcgtg atccagtctc ctagacacga
      481 agtgaccgag atgggccaag aagtgaccct gcgctgcaag cctatcagcg gccacgatta
      541 cctgttctgg tacagacaga ccatgatgag aggcctggaa ctgctgatct acttcaacaa
      601 caacgtgccc atcgacgaca gcggcatgcc cgaggataga ttcagcgcca agatgcccaa
      661 cgccagcttc agcaccctga agatccagcc tagcgagccc agagatagcg ccgtgtactt
      721 ttgtgccagc agcctgggac tgcccggcac cgatacacag tattttggcc ctggcaccag
      781 actgaccgtg ctggaagatc tgaagaacgt gttcccacct gaggtggccg tgttcgagcc
      841 ttctgaggcc gagatcagcc acacacagaa agccacactc gtgtgtctgg ccaccggctt
      901 ctatcccgat cacgtggaac tgtcttggtg ggtcaacggc aaagaggtgc acagcggcgt
      961 ctgtaccgat cctcagcctc tgaaagagca gcccgctctg aacgacagca gatactgcct
     1021 gagcagcaga ctgagagtgt ccgccacctt ctggcagaac cccagaaacc acttcagatg
     1081 ccaggtgcag ttctacggcc tgagcgagaa cgatgagtgg acccaggata gagccaagcc
     1141 tgtgacacag atcgtgtctg ccgaagcctg gggcagagcc gattgtggct ttaccagcga
     1201 gagctaccag cagggcgtgc tgtctgccac aatcctgtac gagatcctgc tgggcaaagc
     1261 cactctgtac gccgtgctgg tgtctgccct ggtgctgatg gccatggtca agcggaagga
     1321 tagcagaggc ggaagcggcg ccacaaactt ctcactgctg aaacaggccg gcgacgtgga
     1381 agagaaccct ggacctatgc tgctcctgct gatccctgtg ctgggcatga tcttcgccct
     1441 gagagatgct agagcccagt ccgtgtctca gcacaaccac catgtgatcc tgagcgaagc
     1501 cgcctctctg gaactgggct gcaattacag ctacggcggc accgtgaatc tgttttggta
     1561 cgtgcagtac cccggccagc atctccagct gctgctgaag tactttagcg gcgaccctct
     1621 ggtcaagggc atcaagggat tcgaggccga gttcatcaag agcaagttca gcttcaacct
     1681 gcggaagccc agcgtgcagt ggagcgatac agccgagtat ttctgcgccg tgaccgtggt
     1741 caacgccggc aacaacagaa agctgatctg gggcctgggc accagcctgg ctgtgaaccc
     1801 caatattcag aaccccgatc ctgcagtgta ccagctgcgg gacagcaaga gcagcgacaa
     1861 gagcgtgtgc ctgttcaccg acttcgacag ccagaccaac gtgtcccaga gcaaggacag
     1921 cgacgtgtac atcaccgata agtgcgtgct ggacatgcgg agcatggact tcaagagcaa
     1981 cagcgccgtg gcctggtcca acaagagcga cttcgcctgc gccaacgcct tcaacaacag
     2041 cattatcccc gaggacacat tcttcccaag ccccgagagc agctgcgacg tgaagctggt
     2101 ggaaaagagc ttcgagacag acaccaacct gaacttccag aacctcagcg tgatcggctt
     2161 ccggatcctg ctgctgaagg tggccggctt caacctgctg atgaccctgc ggctgtggtc
     2221 cagctgagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     2281 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     2341 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     2401 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     2461 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     2521 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     2581 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     2641 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     2701 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     2761 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     2821 gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     2881 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     2941 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     3001 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3061 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     3121 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     3181 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     3241 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3301 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     3361 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     3421 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     3481 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     3541 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     3601 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     3661 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     3721 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     3781 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     3841 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     3901 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     3961 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4021 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4081 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4141 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     4201 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4261 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4321 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4381 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4441 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4501 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4561 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4621 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4681 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4741 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4801 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4861 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4921 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4981 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5041 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5101 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5161 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5221 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5281 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5341 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5401 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5461 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     5521 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5581 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5641 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5701 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5761 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5821 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5881 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5941 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6001 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6061 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6121 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     6181 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     6241 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     6301 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     6361 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     6421 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     6481 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     6541 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     6601 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6661 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6721 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6781 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     6841 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     6901 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     6961 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     7021 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     7081 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     7141 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     7201 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     7261 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     7321 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     7381 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     7441 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     7501 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     7561 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     7621 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     7681 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     7741 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     7801 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     7861 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     7921 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     7981 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     8041 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     8101 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     8161 tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt
     8221 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     8281 aaaaacaaat tacaaaaatt caaaatttta tcgatcacga gactagcc
//
