LOCUS       sequence-242524-         352 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting 
            human SCGB3A2 locus at the end of the endogenous coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 352)
  AUTHORS   McCauley KB, Alysandratos KD, Jacob A, Hawkins F, Caballero IS, 
            Vedaie M, Yang W, Slovik KJ, Morley M, Carraro G, Kook S, Guttentag 
            SH, Stripp BR, Morrisey EE, Kotton DN
  TITLE     Single-Cell Transcriptomic Profiling of Pluripotent Stem 
            Cell-Derived SCGB3A2+ Airway Epithelium.
  JOURNAL   Stem Cell Reports. 2018 May 8;10(5):1579-1595. doi: 
            10.1016/j.stemcr.2018.03.013. Epub 2018 Apr 12.
  PUBMED    29657097
REFERENCE   2  (bases 1 to 352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2018.03.013"; journalName: "Stem Cell Reports"; 
            date: "2018-05-8- 8"; volume: "10"; issue: "5"; pages: "1579-1595"
COMMENT     Sequence Label: pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..352
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        271..346
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg tcaggaggcg ctatcacact gttttagagc tagaaatagc aagttaaaat
      301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tt
//
