LOCUS       sequence-242530-        1179 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral or plasmid based co-expression of Cre recombinase and a 
            neomycin resistance cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Jacob A, Morley M, Hawkins F, McCauley KB, Jean JC, Heins H, Na CL, 
            Weaver TE, Vedaie M, Hurley K, Hinds A, Russo SJ, Kook S, Zacharias 
            W, Ochs M, Traber K, Quinton LJ, Crane A, Davis BR, White FV, 
            Wambach J, Whitsett JA, Cole FS, Morrisey EE, Guttentag SH, Beers 
            MF, Kotton DN
  TITLE     Differentiation of Human Pluripotent Stem Cells into Functional Lung
            Alveolar Epithelial Cells.
  JOURNAL   Cell Stem Cell. 2017 Oct 5;21(4):472-488.e10. doi: 
            10.1016/j.stem.2017.08.014. Epub 2017 Sep 28.
  PUBMED    28965766
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2017.08.014"; journalName: "Cell Stem Cell"; date: 
            "2017-10-5- 5"; volume: "21"; issue: "4"; pages: "472-488.e10"
COMMENT     Sequence Label: pHAGE2-EF1aL-Cre-IRES-NeoR-W
FEATURES             Location/Qualifiers
     source          1..1179
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtga
//
