LOCUS       sequence-242531-        1600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral or plasmid based co-expression of Cre recombinase and a 
            neomycin resistance cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Jacob A, Morley M, Hawkins F, McCauley KB, Jean JC, Heins H, Na CL, 
            Weaver TE, Vedaie M, Hurley K, Hinds A, Russo SJ, Kook S, Zacharias 
            W, Ochs M, Traber K, Quinton LJ, Crane A, Davis BR, White FV, 
            Wambach J, Whitsett JA, Cole FS, Morrisey EE, Guttentag SH, Beers 
            MF, Kotton DN
  TITLE     Differentiation of Human Pluripotent Stem Cells into Functional Lung
            Alveolar Epithelial Cells.
  JOURNAL   Cell Stem Cell. 2017 Oct 5;21(4):472-488.e10. doi: 
            10.1016/j.stem.2017.08.014. Epub 2017 Sep 28.
  PUBMED    28965766
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2017.08.014"; journalName: "Cell Stem Cell"; date: 
            "2017-10-5- 5"; volume: "21"; issue: "4"; pages: "472-488.e10"
COMMENT     Sequence Label: pHAGE2-EF1aL-Cre-IRES-NeoR-W
FEATURES             Location/Qualifiers
     source          1..1600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1029
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    1033..1600
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1194..1211)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1421..1440
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat
       61 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc
      121 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg
      181 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc
      241 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg
      301 tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga
      361 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat
      421 ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg
      481 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg
      541 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa
      601 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc
      661 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg
      721 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg
      781 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag
      841 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc
      901 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc
      961 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat
     1021 ggcgattaga gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     1081 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     1141 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     1201 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     1261 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     1321 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     1381 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     1441 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     1501 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     1561 ccacggggac gtggttttcc tttgaaaaac acgatgataa
//
