LOCUS       sequence-242576-       11719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CA E542K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11719)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CA-E542K
FEATURES             Location/Qualifiers
     source          1..11719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6656..6680)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6733..6774
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6795..7362
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6812..7364
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6956..6973)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7183..7202
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7376..7378,7379..7381,7382..8095)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7376..7378,7379..7381,7382..8092)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7421..7442)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7682..7701)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8029..8050
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8109..8697
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8162..8182)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8580..8591)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8772..9005
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9124..9142)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9210..9314
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9315..9383,9384..10175)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9533..9552)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10346..10934
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10835..10854
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11088..11105
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11345..11366
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11381..11386,11387..11404,11405..11411)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11419..11435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11424..11446
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11443..11459
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11443..11459
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11509..11528
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg cctccaagac catcatcagg tgaactgtgg
     3481 ggcatccact tgatgccccc aagaatccta gtagaatgtt tactaccaaa tggaatgata
     3541 gtgactttag aatgcctccg tgaggctaca ttaataacca taaagcatga actatttaaa
     3601 gaagcaagaa aataccccct ccatcaactt cttcaagatg aatcttctta cattttcgta
     3661 agtgttactc aagaagcaga aagggaagaa ttttttgatg aaacaagacg actttgtgac
     3721 cttcggcttt ttcaaccctt tttaaaagta attgaaccag taggcaaccg tgaagaaaag
     3781 atcctcaatc gagaaattgg ttttgctatc ggcatgccag tgtgtgaatt tgatatggtt
     3841 aaagatccag aagtacagga cttccgaaga aatattctga acgtttgtaa agaagctgtg
     3901 gatcttaggg acctcaattc acctcatagt agagcaatgt atgtctatcc tccaaatgta
     3961 gaatcttcac cagaattgcc aaagcacata tataataaat tagataaagg gcaaataata
     4021 gtggtgatct gggtaatagt ttctccaaat aatgacaagc agaagtatac tctgaaaatc
     4081 aaccatgact gtgtaccaga acaagtaatt gctgaagcaa tcaggaaaaa aactcgaagt
     4141 atgttgctat cctctgaaca actaaaactc tgtgttttag aatatcaggg caagtatatt
     4201 ttaaaagtgt gtggatgtga tgaatacttc ctagaaaaat atcctctgag tcagtataag
     4261 tatataagaa gctgtataat gcttgggagg atgcccaatt tgatgttgat ggctaaagaa
     4321 agcctttatt ctcaactgcc aatggactgt tttacaatgc catcttattc cagacgcatt
     4381 tccacagcta caccatatat gaatggagaa acatctacaa aatccctttg ggttataaat
     4441 agtgcactca gaataaaaat tctttgtgca acctacgtga atgtaaatat tcgagacatt
     4501 gataagatct atgttcgaac aggtatctac catggaggag aacccttatg tgacaatgtg
     4561 aacactcaaa gagtaccttg ttccaatccc aggtggaatg aatggctgaa ttatgatata
     4621 tacattcctg atcttcctcg tgctgctcga ctttgccttt ccatttgctc tgttaaaggc
     4681 cgaaagggtg ctaaagagga acactgtcca ttggcatggg gaaatataaa cttgtttgat
     4741 tacacagaca ctctagtatc tggaaaaatg gctttgaatc tttggccagt acctcatgga
     4801 ttagaagatt tgctgaaccc tattggtgtt actggatcaa atccaaataa agaaactcca
     4861 tgcttagagt tggagtttga ctggttcagc agtgtggtaa agttcccaga tatgtcagtg
     4921 attgaagagc atgccaattg gtctgtatcc cgagaagcag gatttagcta ttcccacgca
     4981 ggactgagta acagactagc tagagacaat gaattaaggg aaaatgacaa agaacagctc
     5041 aaagcaattt ctacacgaga tcctctctct aaaatcactg agcaggagaa agattttcta
     5101 tggagtcaca gacactattg tgtaactatc cccgaaattc tacccaaatt gcttctgtct
     5161 gttaaatgga attctagaga tgaagtagcc cagatgtatt gcttggtaaa agattggcct
     5221 ccaatcaaac ctgaacaggc tatggaactt ctggactgta attacccaga tcctatggtt
     5281 cgaggttttg ctgttcggtg cttggaaaaa tatttaacag atgacaaact ttctcagtat
     5341 ttaattcagc tagtacaggt cctaaaatat gaacaatatt tggataactt gcttgtgaga
     5401 tttttactga agaaagcatt gactaatcaa aggattgggc actttttctt ttggcattta
     5461 aaatctgaga tgcacaataa aacagttagc cagaggtttg gcctgctttt ggagtcctat
     5521 tgtcgtgcat gtgggatgta tttgaagcac ctgaataggc aagtcgaggc aatggaaaag
     5581 ctcattaact taactgacat tctcaaacag gagaagaagg atgaaacaca aaaggtacag
     5641 atgaagtttt tagttgagca aatgaggcga ccagatttca tggatgctct acagggcttt
     5701 ctgtctcctc taaaccctgc tcatcaacta ggaaacctca ggcttgaaga gtgtcgaatt
     5761 atgtcctctg caaaaaggcc actgtggttg aattgggaga acccagacat catgtcagag
     5821 ttactgtttc agaacaatga gatcatcttt aaaaatgggg atgatttacg gcaagatatg
     5881 ctaacacttc aaattattcg tattatggaa aatatctggc aaaatcaagg tcttgatctt
     5941 cgaatgttac cttatggttg tctgtcaatc ggtgactgtg tgggacttat tgaggtggtg
     6001 cgaaattctc acactattat gcaaattcag tgcaaaggcg gcttgaaagg tgcactgcag
     6061 ttcaacagcc acacactaca tcagtggctc aaagacaaga acaaaggaga aatatatgat
     6121 gcagccattg acctgtttac acgttcatgt gctggatact gtgtagctac cttcattttg
     6181 ggaattggag atcgtcacaa tagtaacatc atggtgaaag acgatggaca actgtttcat
     6241 atagattttg gacacttttt ggatcacaag aagaaaaaat ttggttataa acgagaacgt
     6301 gtgccatttg ttttgacaca ggatttctta atagtgatta gtaaaggagc ccaagaatgc
     6361 acaaagacaa gagaatttga gaggtttcag gagatgtgtt acaaggctta tctagctatt
     6421 cgacagcatg ccaatctctt cataaatctt ttctcaatga tgcttggctc tggaatgcca
     6481 gaactacaat cttttgatga cattgcatac attcgaaaga ccctagcctt agataaaact
     6541 gagcaagagg ctttggagta tttcatgaaa caaatgaatg atgcacatca tggtggctgg
     6601 acaacaaaaa tggattggat cttccacaca attaaacagc atgcattgaa ctagaaccca
     6661 gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc
     6721 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
     6781 ggttagtaat gagatccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     6841 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     6901 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     6961 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     7021 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     7081 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     7141 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     7201 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     7261 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     7321 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca cacatatggt
     7381 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     7441 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     7501 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     7561 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     7621 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     7681 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     7741 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     7801 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     7861 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     7921 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     7981 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     8041 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaatcga
     8101 tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     8161 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     8221 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     8281 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     8341 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     8401 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     8461 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     8521 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     8581 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     8641 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     8701 agatccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     8761 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     8821 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8881 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8941 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9001 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     9061 atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa
     9121 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     9181 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9241 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     9301 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     9361 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     9421 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     9481 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     9541 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     9601 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     9661 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     9721 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     9781 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     9841 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     9901 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     9961 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    10021 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10081 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10141 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    10201 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    10261 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10321 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    10381 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    10441 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    10501 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    10561 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    10621 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    10681 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    10741 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    10801 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    10861 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    10921 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10981 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    11041 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    11101 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    11161 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    11221 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
    11281 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    11341 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    11401 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
    11461 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    11521 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    11581 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
    11641 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    11701 gtactacaaa cttagtagt
//
