LOCUS       sequence-242613-       12512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast plasmid overexpressing CPR and sLovA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12512)
  AUTHORS   Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang
            Y, Cai M
  TITLE     Engineered ethanol-driven biosynthetic system for improving 
            production of acetyl-CoA derived drugs in Crabtree-negative yeast.
  JOURNAL   Metab Eng. 2019 Jul;54:275-284. doi: 10.1016/j.ymben.2019.05.001. 
            Epub 2019 May 9.
  PUBMED    31077813
REFERENCE   2  (bases 1 to 12512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-07"; volume: "54:275-284. doi"; pages: " 
            10.1016/j.ymben.2019.05.001. Epub 2019 May 9"
COMMENT     Sequence Label: pK_lacO-cP-AOX1-sAR
FEATURES             Location/Qualifiers
     source          1..12512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    121..137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1910..1927
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2007..2253
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     protein_bind    2269..2285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4559..4576
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4656..4902
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(5321..7855)
                     /codon_start=1
                     /gene="Pichia pastoris HIS4"
                     /product="multifunctional enzyme, required for histidine 
                     biosynthesis"
                     /label=PpHIS4
                     /note="auxotrophic marker for Pichia pastoris"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE
                     LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL
                     VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII
                     DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI
                     PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER
                     TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED
                     IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP
                     KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE
                     LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP
                     GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE
                     VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA
                     LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED
                     AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV
                     QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK
                     HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
     CDS             complement(8269..9084)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8269..9084)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8992..9011
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9076..9095
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(9249..9268)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    9463..10219
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     primer_bind     complement(10254..10276)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10362..10502
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10417..10436
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10517..10534)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10688..11276)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10768..10787)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11447..12238,12239..12307))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12070..12089
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12308..12412)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12480..12498
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaattaattc tcatgtttga cagcttatca tcgataagct gactcatgtt ggtattgtga
       61 aatagacgca gatcgggaac actgaaaaat aacagttatt attcggagct ctgtgtggaa
      121 ttgtgagcgg ataacaattt cacacactaa cccctacttg acagcaatat ataaacagaa
      181 ggaagctgcc ctgtcttaaa cctttttttt tatcatcatt attagcttac tttcataatt
      241 gcgactggtt ccaattgaca agcttttgat tttaacgact tttaacgaca acttgagaag
      301 atcaaaaaac aactaattat tcgaaatgac tgttgacgct ttgactcagc cacaccactt
      361 gttgtccttg gcctggaacg atactcaaca gcacggttcc tggtttgccc ctcttgtcac
      421 tacctccgcc ggtttgttgt gtttgttgtt gtacctttgt tcctccggta gaagatccga
      481 cttgcctgtc ttcaatccta agacctggtg ggagttgacc actatgagag ctaagcgtga
      541 tttcgatgct aacgccccat cctggattga gtcctggttc tcccagaacg acaagccaat
      601 cagatttatt gttgactctg gttactgcac catcttgcct tcctctatgg ctgacgagtt
      661 ccgtaagatg aaggagttgt gcatgtacaa gttcttgggt actgacttcc actcccacct
      721 tccaggtttt gatggattta aagaggtcac cagagacgcc catttgatca ccaaggttgt
      781 catgaaccag ttccagaccc aggccccaaa gtatgtcaag ccattggcca acgaggcctc
      841 cggaattatc accgacatct tcggtgactc taacgagtgg cataccgtcc cagtctacaa
      901 ccagtgcttg gacttggtca ctcgtaccgt caccttcatt atggtcggat ctaagttggc
      961 tcacaacgaa gagtggttgg acatcgccaa gcatcacgcc gttaccatgg ctatccaggc
     1021 cagacagttg cgtttgtggc cagtcatttt gcgtccattg gttcactggc ttgaacctca
     1081 aggagccaag ttgagagctc aagttagaag agctagacaa ttgttggatc caattattca
     1141 agaacgtaga gctgagcgtg atgcttgcag agctaagggt attgagccac ctagatacgt
     1201 cgattccatt cagtggttcg aggacactgc caagggaaaa tggtacgacg ctgctggagc
     1261 tcaattggct atggatttcg ccggaatcta cggtacctct gacttgttga tcggtggatt
     1321 ggttgatatc gttcgtcacc ctcacttgtt ggagccattg cgtgacgaga tccgtaccgt
     1381 tattggtcag ggtggttgga ccccagcctc tttgtacaag ttgaagttgc ttgactcctg
     1441 tcttaaggag tctcagcgtg ttaagccagt cgagtgtgcc accatgagat cttacgcttt
     1501 gcaggacgtc accttctcca acggtacctt catccctaag ggagaattgg tcgccgttgc
     1561 cgccgacaga atgtccaatc ctgaggtctg gccagaacca gccaagtacg acccttaccg
     1621 ttacatgaga ttgcgtgagg acccagctaa ggccttctct gcccagttgg agaacaccaa
     1681 cggtgaccat atcggtttcg gttggcatcc tagagcttgt cctggtcgtt tcttcgcctc
     1741 taaggagatc aaaatgatgc ttgcctattt gttgattaga tacgactgga aggtcgtccc
     1801 agacgaacca ttgcagtact acagacactc cttttccgtc cgtatccatc ctaccaccaa
     1861 gttgatgatg cgtcgtcgtg acgaggacat tagattgcca ggttctttgc atcatcatca
     1921 tcatcattga gtttgtagcc ttagacatga ctgttcctca gttcaagttg ggcacttacg
     1981 agaagaccgg tcttgctaga ttctaatcaa gaggatgtca gaatgccatt tgcctgagag
     2041 atgcaggctt catttttgat acttttttat ttgtaaccta tatagtatag gatttttttt
     2101 gtcattttgt ttcttctcgt acgagcttgc tcctgatcag cctatctcgc agctgatgaa
     2161 tatcttgtgg taggggtttg ggaaaatcat tcgagtttga tgtttttctt ggtatttccc
     2221 actcctcttc agagtacaga agattaagtg agaagatctt gtgtggaatt gtgagcggat
     2281 aacaatttca cacactaacc cctacttgac agcaatatat aaacagaagg aagctgccct
     2341 gtcttaaacc ttttttttta tcatcattat tagcttactt tcataattgc gactggttcc
     2401 aattgacaag cttttgattt taacgacttt taacgacaac ttgagaagat caaaaaacaa
     2461 ctaattattc gaaatggctc aactcgacac tctcgacctg gtggtcctgg tggtgctttt
     2521 ggtgggtagc gccgcctact tcaccaaggg cacctactgg gccgttccca aggacccgta
     2581 tgccgcctcc ggtcccgcca tgaatggtgg cgccaaggcg ggcaaatcca gggacatcat
     2641 tgagaaaatg gaagagactg gcaagaactg tgtgattttc tacggctcgc agaccggtac
     2701 cgccgaggat tatgcgtcgc gcctggccaa ggaaggctcc cagcgtttcg gcctcaagac
     2761 catggtcgca gatctggaag actacgatta tgagaacctg gacaagttcc ccgaggacaa
     2821 ggttgccttc ttcgtcatgg ccacctatgg tgagggtgaa cccaccgaca acgccgtcga
     2881 gttctaccag ttcatctcgg gtgaggacgt cgcgttcgag agcggcgcct ctgccgacga
     2941 caagcccctg tcctccctca agtatgtcac tttcggtctc ggtaacaaca cctatgagca
     3001 ctatcaggct atggttcgca atctggatgc cgctctcacc aagctgggtg cgcagcgcat
     3061 tggagatgct ggtgaaggtg atgacggcgc tggcaccatg gaagaagatt tcctggcctg
     3121 gaaagagccc atgtggactg ccctgtccga ggccatgaac cttcaggagc gcgaagccgt
     3181 ctatgagccg gtgttctcgg tcacggaaga tgaatccctg tcccccgaag acgaagccgt
     3241 ctacctcggt gagccgacca agggtcaccg tgacggcacc cccagtggcc cgtattccgc
     3301 tcacaacccc ttcatcgccc ccatcgtcga gtctcgtgaa ctgttcaacg tcaaggaccg
     3361 taattgtctg cacatggaga tcagcatcgc tggtagcaac ctttcttacc agactggtga
     3421 tcacatcgcg atttggccca cgaacgctgg tgccgaggtg gaccggttcc tccaggtgtt
     3481 tggtcttgag aacaagcgtc attccgtcat caacatcaag ggtatcgatg tgaccgccaa
     3541 ggttcccatt ccgactccca ccacgtatga tgctgctgtt cgctactata tggaaattgc
     3601 tgcgcccgtc tcccgtcagt ttgtggctac cctggctgcg tttgctcccg atgaggagac
     3661 taaggcggaa atcgtgcgtt tgggtagcga caaggactac ttccacgaga aaatcagcaa
     3721 ccagtgcttc accatcgctc aggctcttca gagtgtcacc tccaagccct tctcggctgt
     3781 cccgttctct ctgcttatcg agggtctcaa taagctccag ccccgttact actccatctc
     3841 ttcctcctcc atggtccaga aggataagat cagcattact gccgtcgtgg aatccactcg
     3901 cttgcctggt gccgcccacc ttgtcaaggg tgtcacgacc aactatctcc ttgccctgaa
     3961 gcaaaagcag aatggggatc cgtctcccga ccctcacggc ttaacttata ctatcactgg
     4021 gccccgtaac aagtacgacg gaatccacgt tcccgttcac gtccgccact ccaatttcaa
     4081 gctcccctct gatccctctc ggcccattat catggttggc cctggtaccg gtgtggctcc
     4141 cttccgtgga ttcatccagg agcgtgccgc cttggccgcc aagggtgaga acgtcggtcc
     4201 caccgtgttg ttctttggat gccgcaggcg cgatgaggac tttatgtacg cagatgaatt
     4261 caagacctac caggaacagc ttggggacaa gcttcagatc attactgcgt tttctcgtga
     4321 aacttcccag aaggtgtatg ttcagcacag actgcgtgaa cactccgatc tggtgagcag
     4381 cctcctgaag cagaaggcta acttttacgt ctgcggtgac gccgccaaca tggcgcgtga
     4441 agtcaacctt gtgcttggcc agatcatcgc gcaacagcgg ggtctcccgg ctgaacgggc
     4501 cgaggaaatg gtgaagcaca tgcgcagcag cggcagctac caggaggacg tgtggtcaca
     4561 tcatcatcat catcattgag tttgtagcct tagacatgac tgttcctcag ttcaagttgg
     4621 gcacttacga gaagaccggt cttgctagat tctaatcaag aggatgtcag aatgccattt
     4681 gcctgagaga tgcaggcttc atttttgata cttttttatt tgtaacctat atagtatagg
     4741 attttttttg tcattttgtt tcttctcgta cgagcttgct cctgatcagc ctatctcgca
     4801 gctgatgaat atcttgtggt aggggtttgg gaaaatcatt cgagtttgat gtttttcttg
     4861 gtatttccca ctcctcttca gagtacagaa gattaagtga gaactagtag ttcgtttgtg
     4921 caagcttatc gataagcttt aatgcggtag tttatcacag ttaaattgct aacgcagtca
     4981 ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga
     5041 tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca
     5101 ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt
     5161 tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct
     5221 cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg
     5281 gatctatcga atctaaatgt aagttaaaat ctctaaataa ttaaataagt cccagtttct
     5341 ccatacgaac cttaacagca ttgcggtgag catctagacc ttcaacagca gccagatcca
     5401 tcactgcttg gccaatatgt ttcagtccct caggagttac gtcttgtgaa gtgatgaact
     5461 tctggaaggt tgcagtgtta actccgctgt attgacgggc atatccgtac gttggcaaag
     5521 tgtggttggt accggaggag taatctccac aactctctgg agagtaggca ccaacaaaca
     5581 cagatccagc gtgttgtact tgatcaacat aagaagaagc attctcgatt tgcaggatca
     5641 agtgttcagg agcgtactga ttggacattt ccaaagcctg ctcgtaggtt gcaaccgata
     5701 gggttgtaga gtgtgcaata cacttgcgta caatttcaac ccttggcaac tgcacagctt
     5761 ggttgtgaac agcatcttca attctggcaa gctccttgtc tgtcatatcg acagccaaca
     5821 gaatcacctg ggaatcaata ccatgttcag cttgagacag aaggtctgag gcaacgaaat
     5881 ctggatcagc gtatttatca gcaataacta gaacttcaga aggcccagca ggcatgtcaa
     5941 tactacacag ggctgatgtg tcattttgaa ccatcatctt ggcagcagta acgaactggt
     6001 ttcctggacc aaatattttg tcacacttag gaacagtttc tgttccgtaa gccatagcag
     6061 ctactgcctg ggcgcctcct gctagcacga tacacttagc accaaccttg tgggcaacgt
     6121 agatgacttc tggggtaagg gtaccatcct tcttaggtgg agatgcaaaa acaatttctt
     6181 tgcaaccagc aactttggca ggaacaccca gcatcaggga agtggaaggc agaattgcgg
     6241 ttccaccagg aatatagagg ccaactttct caataggtct tgcaaaacga gagcagacta
     6301 caccagggca agtctcaact tgcaacgtct ccgttagttg agcttcatgg aatttcctga
     6361 cgttatctat agagagatca atggctctct taacgttatc tggcaattgc ataagttcct
     6421 ctgggaaagg agcttctaac acaggtgtct tcaaagcgac tccatcaaac ttggcagtta
     6481 gttctaaaag ggctttgtca ccattttgac gaacattgtc gacaattggt ttgactaatt
     6541 ccataatctg ttccgttttc tggataggac gacgaagggc atcttcaatt tcttgtgagg
     6601 aggccttaga aacgtcaatt ttgcacaatt caatacgacc ttcagaaggg acttctttag
     6661 gtttggattc ttctttaggt tgttccttgg tgtatcctgg cttggcatct cctttccttc
     6721 tagtgacctt tagggacttc atatccaggt ttctctccac ctcgtccaac gtcacaccgt
     6781 acttggcaca tctaactaat gcaaaataaa ataagtcagc acattcccag gctatatctt
     6841 ccttggattt agcttctgca agttcatcag cttcctccct aattttagcg ttcaacaaaa
     6901 cttcgtcgtc aaataaccgt ttggtataag aaccttctgg agcattgctc ttacgatccc
     6961 acaaggtggc ttccatggct ctaagaccct ttgattggcc aaaacaggaa gtgcgttcca
     7021 agtgacagaa accaacacct gtttgttcaa ccacaaattt caagcagtct ccatcacaat
     7081 ccaattcgat acccagcaac ttttgagttg ctccagatgt agcaccttta taccacaaac
     7141 cgtgacgacg agattggtag actccagttt gtgtccttat agcctccgga atagactttt
     7201 tggacgagta caccaggccc aacgagtaat tagaagagtc agccaccaaa gtagtgaata
     7261 gaccatcggg gcggtcagta gtcaaagacg ccaacaaaat ttcactgaca gggaactttt
     7321 tgacatcttc agaaagttcg tattcagtag tcaattgccg agcatcaata atggggatta
     7381 taccagaagc aacagtggaa gtcacatcta ccaactttgc ggtctcagaa aaagcataaa
     7441 cagttctact accgccatta gtgaaacttt tcaaatcgcc cagtggagaa gaaaaaggca
     7501 cagcgatact agcattagcg ggcaaggatg caactttatc aaccagggtc ctatagataa
     7561 ccctagcgcc tgggatcatc ctttggacaa ctctttctgc caaatctagg tccaaaatca
     7621 cttcattgat accattattg tacaacttga gcaagttgtc gatcagctcc tcaaattggt
     7681 cctctgtaac ggatgactca acttgcacat taacttgaag ctcagtcgat tgagtgaact
     7741 tgatcaggtt gtgcagctgg tcagcagcat agggaaacac ggcttttcct accaaactca
     7801 aggaattatc aaactctgca acacttgcgt atgcaggtag caagggaaat gtcatacttg
     7861 aagtcggaca gtgagtgtag tcttgagaaa ttctgaagcc gtatttttat tatcagtgag
     7921 tcagtcatca ggagatcctc tacgccggac gcatcgtggc cgacctgcag gggggggggg
     7981 ggcgctgagg tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc
     8041 catcatccag ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc
     8101 agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg
     8161 tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagccgc cgtcccgtca
     8221 agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc
     8281 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     8341 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     8401 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     8461 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     8521 gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc
     8581 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag
     8641 acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
     8701 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     8761 ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
     8821 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     8881 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     8941 atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
     9001 ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga
     9061 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
     9121 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
     9181 cacaacgtgg ctttcccccc cccccctgca ggtcggcatc accggcgcca caggtgcggt
     9241 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     9301 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
     9361 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     9421 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgagtat ctatgattgg
     9481 aagtatggga atggtgatac ccgcattctt cagtgtcttg aggtctccta tcagattatg
     9541 cccaactaaa gcaaccggag gaggagattt catggtaaat ttctctgact tttggtcatc
     9601 agtagactcg aactgtgaga ctatctcggt tatgacagca gaaatgtcct tcttggagac
     9661 agtaaatgaa gtcccaccaa taaagaaatc cttgttatca ggaacaaact tcttgtttcg
     9721 aactttttcg gtgccttgaa ctataaaatg tagagtggat atgtcgggta ggaatggagc
     9781 gggcaaatgc ttaccttctg gaccttcaag aggtatgtag ggtttgtaga tactgatgcc
     9841 aacttcagtg acaacgttgc tatttcgttc aaaccattcc gaatccagag aaatcaaagt
     9901 tgtttgtcta ctattgatcc aagccagtgc ggtcttgaaa ctgacaatag tgtgctcgtg
     9961 ttttgaggtc atctttgtat gaataaatct agtctttgat ctaaataatc ttgacgagcc
    10021 aaggcgataa atacccaaat ctaaaactct tttaaaacgt taaaaggaca agtatgtctg
    10081 cctgtattaa accccaaatc agctcgtagt ctgatcctca tcaacttgag gggcactatc
    10141 ttgttttaga gaaatttgcg gagatgcgat atcgagaaaa aggtacgctg attttaaacg
    10201 tgaaatttat ctcaagatct ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
    10261 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    10321 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
    10381 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
    10441 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    10501 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    10561 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    10621 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    10681 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    10741 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    10801 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    10861 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
    10921 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    10981 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    11041 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    11101 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    11161 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    11221 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    11281 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    11341 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    11401 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    11461 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    11521 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    11581 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    11641 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    11701 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    11761 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    11821 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    11881 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    11941 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    12001 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    12061 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    12121 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    12181 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    12241 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    12301 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    12361 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    12421 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    12481 ataggcgtat cacgaggccc tttcgtcttc aa
//
