LOCUS       sequence-242677-        7569 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  7886 bases long scaffold with Zn-dependent self-cleaving DNAzyme 
            cassettes, from compatibility group 8, made by method IV, yields 
            linear 7569 b long scaffolds after Zn incubation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Engelhardt FAS, Praetorius F, Wachauf CH, Bruggenthies G, Kohler F, 
            Kick B, Kadletz KL, Pham PN, Behler KL, Gerling T, Dietz H
  TITLE     Custom-Size, Functional, and Durable DNA Origami with 
            Design-Specific Scaffolds.
  JOURNAL   ACS Nano. 2019 Apr 22. doi: 10.1021/acsnano.9b01025.
  PUBMED    30990672
REFERENCE   2  (bases 1 to 7569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Nano. 
            2019 Apr 22. doi: 10.1021/acsnano.9b01025."
COMMENT     Sequence Label: pre_IV_CS8_7569_v1
FEATURES             Location/Qualifiers
     source          1..7569
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6925..6936
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aggtgtccaa gctgtcaacg caccaagcat acaagggtgt aagctgagaa cctcataacg
       61 ctagaacatc ataagagcgc aacacgataa tccaggaacc tgacaaggta gtaaccccct
      121 aatcccacaa gagggtaaca cctcaacagg gcaactcgtg aatgtctgaa gtagataacc
      181 tgataagggg ctaagccact aagcccctaa gcgcccaatg gcgtaatgtg acaatacggt
      241 aagtcctcaa gagtccaacg tagcaaccgt cgaacacgct aacatcggaa cactacaata
      301 cgataaccca ggaacgaggg aactagcgaa cagcgtaact gtggaatcgg gcaatgcggt
      361 aatcgatcaa catggtaatc tacgaactcc acaatgcagc aacgacctaa gcagataata
      421 tgagaacgtg gcaacatctg aatggtagaa cgctgcaagg aggtaacgag ctaatccggt
      481 aaggggggaa cggaggaacc gaccaataca tgaaggatgt aatagaggaa gctgacaagg
      541 caccaagtgt cgaagatcgc aatgagggaa tatatcaatc cagtaagggc gcaagcggat
      601 aacatgcgaa cgcctgaatg gggcaaccgc gcaatcggtc aatggtcgaa cgctacaagt
      661 ggacaacagg cgaagtgctc aactgagtaa ggatcgaatc catgaagcca tgaactatct
      721 aatcccgcaa ctccataacg tcgtaagatg cgaagcgtgt aaggtgacaa tatcccaaga
      781 tagcaagtat ataagggcgg aagccaggaa gctggcaacg cgcgaatcgt gtaagtgagt
      841 aagtgcggaa gacacgaacg acacaatgtg ggaacctcag aacgctgtaa cgatccaatc
      901 tgataaccac gtaagtacct aaggcgtgaa cacccgaatg tcagaatgcc tcaatcagag
      961 aatcacctaa tctagcaatc ccccaacgcc gtaaccaccc aagccgcgaa gataccaaga
     1021 gtataatcga ggaatgcgat aacccccgaa gggtggaatc acagaactcg ctaatgctcc
     1081 aagtgcacaa ctctctaact gtgtaagctc gtaatccgcg aacatgccaa ctgtccaacc
     1141 ctccaatatc agaagaggtc aagtctctaa ccgactaacg gtacaatcat agaatgggat
     1201 aacgggctaa gggaccaagt acgtaatcgc acaatgacgt aaggggacaa tgagagaagc
     1261 cgtcaagcct gcaatcggcg aacctaccaa gagatgaact ggggaacgat cgaatgtacc
     1321 aacactataa ggcctgaatg tcccaagcgc ataacacagc aagtgcgtaa tacgcgaact
     1381 gtacaaggcc ggaactctgg aaccaggtaa ccatcgaatg ctgcaacagt agaagctgcc
     1441 aagctgggaa cacgggaagg agataatatg tgaatgagat aacagtccaa gtgatgaagt
     1501 agccaaggcc gcaactctac aagtcagcaa tggcggaaca ccctaactac ccaactcact
     1561 aacccgagaa gggggcaagc ccataacgga gtaatcggag aatctctgaa ccacagaata
     1621 gcctaatatc tcaacacgcg aatgccagaa gagcacaagc atgcaatcga ctaaggacgt
     1681 aacactgtaa tgaggcaatg ccgtaagacc ccaaggccgt aatacgagaa cagggtaact
     1741 gtctaacgag acaagcgcag aactcaccaa gtgtctaagc atggaatatc ataagacctg
     1801 aaggcgcgaa ccagcgaacc atccaacatc gcaacgtgat aatacagtaa ctctccaact
     1861 accgaatctg gcaatcgacg aatcgcgtaa tgacgcaacc gggtaaggtc agaagatgac
     1921 aagctcccaa tgatacaagc agtgaacgag ataagggtcg aatcggctaa caggtgaacc
     1981 atggaactag ggaatggtac aagtcgcgaa gtactgaacg caggaagtga gcaacccgac
     2041 aacatcacaa tcgtctaagg ctcgaatgga tcaatcgctg aactgctcaa gcgccgaatg
     2101 catcaacgac gtaacgggat aatgtgcgaa gcggacaata gtctaatccg agaatcagcg
     2161 aacgccacaa tagctgaaga tggtaagggt acaatctcgt aagtagctaa cgtgtcaacg
     2221 cgataatgag gtaagacgag aacaggacaa taccataata gtgcaatacc acaaggcgac
     2281 aatgtactaa gacggtaagt gtataatcag ctaagcgcgg aacctaggaa tggcctaatg
     2341 tggtaagaca tgaagtatgc aagcgctcaa ggtaccaaga cgataatcga ccaaggtcgt
     2401 aacagcccaa tccctcaacc gctcaaccag agaacatgct aactggcgaa gcccgcaact
     2461 catcaaggtg ccaatgatat aatcgcctaa catggcaacc ggcgaatata gtaacagagt
     2521 aactgcccaa ccggggaagt caccaagcag ggaaggcacg aagtatggaa cgggcgaatc
     2581 ccgtaatagg tcaatctgcc aagagtagaa gcactcaaga cccgaacgtc ataactcgcg
     2641 aatatctgaa cggcacaata ggataaggat ccaaggggag aatacctcaa cgcagcaacc
     2701 gcgtaagtct cgaagacatc aagtaggcaa gcagctaagc gcacaaccta gcaaggatac
     2761 aatgtctcaa ctagctaacg aggtaacgcc ataatcaggt aacccgataa ctggtcaacc
     2821 ccccaagacg cgaagcatct aatgtcggaa cgcgggaatc ggccaaccta tcaatgccgg
     2881 aatgcacgaa cgcgtgaagc tacgaatagg ggaatctcgc aagtggtcaa tactccaacg
     2941 gtctaatact cgaacatagc aatgccgcaa ctagtcaata taccaactga tcaacatctc
     3001 aatgggcgaa gatgtgaacc ccacaatccg ccaagatagt aagtggcgaa ctcaggaatg
     3061 gagtaaggtg cgaatagagt aactccgtaa cgagcgaaga cgggaacccc ataatcgtcg
     3121 aaggcatcaa tcggtgaagg ggccaaggcg gtaagcacac aacaggggaa ggacctaaga
     3181 ggtgaagagc cgaacggtgc aagtgacgaa catgtcaagc acgcaacgct ctaagcgggg
     3241 aatgcggcaa cccagcaacc ctacaagact ccaacacgag aagctgtgaa ggagagaagc
     3301 gatgaactga ccaagtctcc aaccacctaa cgactgaata tactaatgag tcaagcacag
     3361 aagactgtaa ctctgtaagc tagcaactcg gtaatcgggg aacccagtaa tagatgaatg
     3421 agccaaccgc agaatacagg aacctcccaa gcctccaacg ccgcaaggtg gtaacggggc
     3481 aatagtggaa gaccacaacg ccccaacgta tcaaccgtct aatgtacgaa gaccataatc
     3541 gtgcaacagt ataatcatcg aacactccaa ctatcgaagc tgataagacc ggaagtatgt
     3601 aaggcagcaa gtgcgcaatg tgctaaccat ctaagcgtcc aaggctggaa ccggtgaagg
     3661 ccccaacgcg agaaccccgc aacctgctaa caccggaatg atgtaacgcc ctaaggtgtg
     3721 aagatcgtaa gacggcaatg tcgtaacagg tcaagtcggg aacgcggtaa gcggctaact
     3781 gtataacctc cgaatgatgc aactcacgaa cggactaacc ccagaatgga ctaacgtacg
     3841 aaggatctaa gagggcaagg ccagaagggc ataagctgct aagagagtaa gacgccaatc
     3901 tgtgaactga cgaatcatgg aagctggtaa tccggcaagg agcgaacata ctaatcgcgc
     3961 aagagtcgaa gccgacaagt gatcaacgac ggaagggact aactcccgaa ggtcgcaatg
     4021 acagaagcgg cgaataggac aacctggtaa catgagaaga ccagaatagt acaacctagt
     4081 aacactctaa cctatgaata gcataatgcg tcaatacccc aagatcccaa tggagcaaca
     4141 ccagaagatc agaagcaggc aatgatctaa tgactcaacg ctcgaacctg agaagtggcc
     4201 aatatagcaa cgtatgaatc atataatagt agaatagatc aacgatacaa gactgcaatc
     4261 gtagaatcgc ataacaccat aagcacggaa gtgctgaaca caggaatacg gcaacccgcg
     4321 aatcggataa cacacgaaca gctcaagctc ggaagtcgcc aacctctgaa gcagacaagg
     4381 cactaatcag ggaacgcctc aagggaggaa cggggtaaga cctcaacctg tgaacccctg
     4441 aacccgccaa cgatctaacc gacgaagatg ctaagtcacg aatatgctaa ggtctgaatc
     4501 cgacaatgag ctaacagagg aacaggctaa catgataacg cgctaatcct ccaatacgtg
     4561 aatgccataa cgtctgaatg ccccaagctc cgaatctgac aaggacgcaa tctctcaaga
     4621 caccaagtcc cgaatacgtc aagtccacaa ggccctaagt ggtgaatgac ggaatggtgt
     4681 aatacaccaa ccatgcaatg cgcgaatgct acaagggcac aagtacccaa cggatgaact
     4741 aggcaacagc agaacctgcg aagatgggaa ccacacaagt gaggaactcg agaatgctgg
     4801 aacgacataa tgtatgaacc cactaactca gcaacaggcc aagccctgaa gatggcaacg
     4861 tgctaagtgc cgaatccctg aagagcataa tgagcgaagg caggaatcac gtaactagat
     4921 aactcgataa gggatgaact gatgaagcga ctaatcagac aacgcgtcaa cgtcggaatc
     4981 gtccaaccct gtaagtgcct aacaccgcaa catcagaact acctaagata ctaacggtat
     5041 aagacgacaa tggtataata ccagaactgc cgaagctgcg aaggagtgaa tagggcaacc
     5101 actcaacaga gcaaggacac aagcatccaa gccggcaagc agccaatgga cgaatcgcag
     5161 aacggcagaa gctcataagt cggtaatgcc cgaactccct aacggcataa cacgtgaaga
     5221 tcacaatgtg gcaaggcctc aacaccccaa tgctgtaacg cactaagcat gtaagagtgt
     5281 aatgtgtgaa gcgagcaagc ccccaaggta tcaatagtat aactggagaa gcctgtaacc
     5341 gagtaatata tgaatcagtc aagagtctaa tagtcgaagc gagtaacgta ctaagtagcg
     5401 aactacataa tgcgctaatc tgagaatcgc cgaatacatc aacgtcccaa ccactgaacg
     5461 tcgcaatatg cgaatacccg aatcaccgaa cacggcaaca ctcgaatctg cgaagcggtc
     5521 aaggtgataa ctcgtcaaca ggataagcgc ctaagctcct aagacgctaa gataggaatg
     5581 cctgaatagc agaatcgcgg aagcctacaa tgacccaagg cggcaatcgg gtaatcccgg
     5641 aaccggagaa tgctataagt ctacaagtag gtaatctgct aacacagtaa tgcatgaagt
     5701 atacaaggag ggaaggacag aatgggacaa ccgatgaagc tagtaagcga ggaatgcagt
     5761 aagctctcaa gtgtagaact agccaagtcc gcaactctgc aagatatgaa gaccgcaatg
     5821 gcgcaacgtg acaatggctg aactatgcaa cgcgccaacc ctctaagagc ccaacggcgg
     5881 aatatcctaa ccgtataatc cgataagcgt agaactgcag aatctcataa cgtggtaacc
     5941 gcctaagtgg agaatactgt aactagagaa ccaccgaatg gcataatcct ctaactaggt
     6001 aaggcgctaa ctgaggaacc ccgtaaggtg gcaagcagcg aatctagtaa ggtcctaaga
     6061 gaggaacggc cgaactgact aactagacaa cagcgcaagt cggcaagcag tcaagggctc
     6121 aataggctaa tatgggaagc ccggaagtga ccaagatctc aatccgtgaa ccatacaact
     6181 gcataatggc cgaagtccgg aacgccggaa gaggctaagc atagaatacg ccaatgtgag
     6241 aaggtagcaa ccatataacc gcccaaccgt acaactcatg aatgccacaa catgacaagg
     6301 actcaaccta cgaactactc aagtccataa ccgaggaacg gatcaaggga gcaataggcg
     6361 aaggtgtcaa cgtgtgaatg tagcaagtac gcaagatcgg aatctcggaa cagactaagg
     6421 catgaagatg agaatgggtc aagactggaa taccggaacg agccaaccgt gtaagcgtat
     6481 aatgtgataa ctgtagaacg cggcaatgag acaaccagat aatgcaggaa gctatcaagc
     6541 tcagaagagg cgaatgcacc aagctactaa tctccgaaca ccgtaacgtg ggaataccgc
     6601 aacccgctaa tgcactaagc acctaagagg ataacccctc aatacgacaa gtagacaagc
     6661 taggaatctc ccaagcctct aatagggtaa gactcgaact acggaagccc tcaagtccct
     6721 aacgagtgaa cagtcgaagt acggaacgct ccaatgagtg aatccactaa cccacgaagt
     6781 atctaaggac ataatatgcc aatgcgccaa ctgcctaatg tagtaacgac ccaacatcct
     6841 aagtcagtaa gtgtacaaga tgtcaatcca gcaacacggt aagagcgtaa cggctcaaga
     6901 gcggaaggcc acaacatacc aaccatagaa ggtcggaaga gagcaagcga tcaagacgtc
     6961 aacatcgtaa tcatgtaaga gatcaactgg gtaagtccag aaccctcgaa tcgtggaact
     7021 cggcaacacc tgaatgggct aacccatcaa gagtggaagt caggaagatc cgaacgtgag
     7081 aatatcgtaa gagctgaagc accgaaggcg ataatatcgg aaggcgggaa gcgacgaaga
     7141 cactaagtgc ataatcgtat aactatgtaa gatatcaagg cgccaagtgg gtaacggtgt
     7201 aagtagtgaa ccggacaatg atagaactac agaagtgcag aactatagaa tcgctcaacc
     7261 gctgaatacc tgaagccggg aagctcacaa gggtctaagc gggcaactga gcaagtcatg
     7321 aagcgggtaa cgatagaaga gactaaccag ccaacgtccg aagtgtgtaa taccctaata
     7381 ggtgaaggac ccaactagtg aacggcccaa cagcctaacc tggcaacgtg ccaaggtact
     7441 aagtgactaa tggtctaagc gcgtaagtct agaatctggt aagtacagaa gcacccaacc
     7501 ggtcaacata tgaagtctgc aaggatggaa ccggccaacc tcacaactgt gcaatcactg
     7561 aaacgttga
//
