LOCUS       sequence-242771-        8363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Moon SH, Lin L, Zhang X, Nguyen TA, Darlington Y, Waldman AS, Lu X, 
            Donehower LA
  TITLE     Wild-type p53-induced phosphatase 1 dephosphorylates histone variant
            gamma-H2AX and suppresses DNA double strand break repair.
  JOURNAL   J Biol Chem. 2010 Apr 23. 285(17):12935-47.
  PUBMED    20118229
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Apr 23. 285(17):12935-47."
COMMENT     Sequence Label: hWIP1 FLAG
FEATURES             Location/Qualifiers
     source          1..8363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(41..60)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     651..670
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(975..994)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1019..1066
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1359..1738
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1739..1942
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1893..1913
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1939..1963
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2054..2626
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             4521..4544
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4643..4662)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4708..4763
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4762..4781)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5121..5137)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5121..5137)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5134..5156)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5145..5161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5169..5175,5176..5193,5194..5199))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5214..5235
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5352..5369)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5523..6111)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5603..5622)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6282..7073,7074..7142))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6905..6924
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7143..7247)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7315..7333
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7371..7393)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7493..7512
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7706..7728
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7720..7737
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7721..7737
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8148..8293
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             join(8350..8363,1..781)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
       61 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
      121 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
      181 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
      241 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
      301 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
      361 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
      421 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
      481 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
      541 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
      601 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
      661 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
      721 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
      781 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
      841 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
      901 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccgggaga
      961 tgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     1021 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     1081 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     1141 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     1201 gccaacgtcg gggcggcaag ccctgccata gccacgggcc ccgtgggtta gggacggcgg
     1261 atcgcggccc tctagagagc ttggcccatt gcatacgttg tatccatatc ataatatgta
     1321 catttatatt ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta
     1381 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     1441 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     1501 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     1561 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     1621 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     1681 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     1741 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     1801 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     1861 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     1921 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
     1981 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccggt cgaccgatcc
     2041 tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa
     2101 attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc
     2161 cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca
     2221 accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt
     2281 gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct
     2341 ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta
     2401 gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa attctggctg
     2461 gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc tgcctttctc
     2521 tttatggtta caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc
     2581 gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc tgggcaacgt
     2641 gctggttgtt gtgctgtctc atcattttgg caaagaattc ctcgacggat cccggccagc
     2701 cggccatggc ggggctgtac tcgctgggag tgagcgtctt ctccgaccag ggcgggagga
     2761 agtacatgga ggacgttact caaatcgttg tggagcccga accgacggct gaagaaaagc
     2821 cctcgccgcg gcggtcgctg tctcagccgt tgcctccgcg gccgtcgccg gccgcccttc
     2881 ccggcggcga agtctcgggg aaaggcccag cggtggcagc ccgagaggct cgcgaccctc
     2941 tcccggacgc cggggcctcg ccggcaccta gccgctgctg ccgccgccgt tcctccgtgg
     3001 cctttttcgc cgtgtgcgac gggcacggcg ggcgggaggc ggcacagttt gcccgggagc
     3061 acttgtgggg tttcatcaag aagcagaagg gtttcacctc gtccgagccg gctaaggttt
     3121 gcgctgccat ccgcaaaggc tttctcgctt gtcaccttgc catgtggaag aaactggcgg
     3181 aatggccaaa gactatgacg ggtcttccta gcacatcagg gacaactgcc agtgtggtca
     3241 tcattcgggg catgaagatg tatgtagctc acgtaggtga ctcaggggtg gttcttggaa
     3301 ttcaggatga cccgaaggat gactttgtca gagctgtgga ggtgacacag gaccataagc
     3361 cagaacttcc caaggaaaga gaacgaatcg aaggacttgg tgggagtgta atgaacaagt
     3421 ctggggtgaa tcgtgtagtt tggaaacgac ctcgactcac tcacaatgga cctgttagaa
     3481 ggagcacagt tattgaccag attccttttc tggcagtagc aagagcactt ggtgatttgt
     3541 ggagctatga tttcttcagt ggtgaatttg tggtgtcacc tgaaccagac acaagtgtcc
     3601 acactcttga ccctcagaag cacaagtata ttatattggg gagtgatgga ctttggaata
     3661 tgattccacc acaagatgcc atctcaatgt gccaggacca agaggagaaa aaatacctga
     3721 tgggtgagca tggacaatct tgtgccaaaa tgcttgtgaa tcgagcattg ggccgctgga
     3781 ggcagcgtat gctccgagca gataacacta gtgccatagt aatctgcatc tctccagaag
     3841 tggacaatca gggaaacttt accaatgaag atgagttata cctgaacctg actgacagcc
     3901 cttcctataa tagtcaagaa acctgtgtga tgactccttc cccatgttct acaccaccag
     3961 tcaagtcact ggaggaagat ccatggccaa gggtgaattc taaggaccat atacctgccc
     4021 tggttcgtag caatgccttc tcagagaatt ttttagaggt ttcagctgag atagctcgag
     4081 agaatgtcca aggtgtagtc ataccctcaa aagatccaga accacttgaa gaaaattgcg
     4141 ctaaagccct gactttaagg atacatgatt ctttgaataa tagccttcca attggccttg
     4201 tgcctactaa ttcaacaaac actgtcatgg accaaaaaaa tttgaagatg tcaactcctg
     4261 gccaaatgaa agcccaagaa attgaaagaa cccctccaac aaactttaaa aggacattag
     4321 aagagtccaa ttctggcccc ctgatgaaga agcatagacg aaatggctta agtcgaagta
     4381 gtggtgctca gcctgcaagt ctccccacaa cctcacagcg aaagaactct gttaaactca
     4441 ccatgcgacg cagacttagg ggccagaaga aaattggaaa tcctttactt catcaacaca
     4501 ggaaaactgt ttgtgtttgc gactacaagg acgacgatga caagtgagga tccgtcgagg
     4561 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     4621 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     4681 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     4741 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     4801 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     4861 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     4921 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     4981 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     5041 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacggat
     5101 cggccgcaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     5161 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     5221 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     5281 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5341 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5401 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5461 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5521 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5581 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5641 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5701 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5761 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5821 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5881 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     5941 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6001 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6061 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6121 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6181 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6241 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6301 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6361 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6421 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6481 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6541 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6601 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6661 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6721 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6781 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6841 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6901 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6961 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7021 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7081 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7141 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7201 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7261 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     7321 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     7381 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     7441 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     7501 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     7561 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     7621 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     7681 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
     7741 aagcttcacg ctgccgcaag cactcagggc gcaagggctg ctaaaggaag cggaacacgt
     7801 agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct
     7861 ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc
     7921 gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc
     7981 cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga
     8041 tctgatggcg caggggatca agatcctgct tcatccccgt ggcccgttgc tcgcgtttgc
     8101 tggcggtgtc cccggaagaa atatatttgc atgtctttag ttctatgatg acacaaaccc
     8161 cgcccagcgt cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc
     8221 ccaggtccac ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca
     8281 gcgacccgct taacagcgtc aacagcgtgc cgcagatctg atcaagagac aggatgagga
     8341 tcgtttcgca tgattgaaca aga
//
