LOCUS       sequence-243241-       11812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV replicon with constitutive Luc expression, sgRNA targeting 
            PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11812)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 11812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM117
FEATURES             Location/Qualifiers
     source          1..11812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1314..1338
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2638..3267
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3701..4768
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      4834..5028
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5264..5286)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5372..5512
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5427..5446
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5527..5544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5698..6286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5778..5797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6373..7167)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7592..7616
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        8557..8902
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8845..8865
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             8920..10572
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(8986..9004)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      10582..10830
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        11323..11398
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ccttttattt tcttcttttt tccatattta gggttgacag tgaaatcaga ctggcaacct
       61 attaattgct tccacaatgg gacgaacttg aaggggatgt cgtcgatgat attataggtg
      121 gcgtgttcat cgtagttggt gaaatcgatg gtaccgttcc aatagttgtg tcgtccgaga
      181 cttctagccc aggtggtctt tccggtacga gttggtccgc agatgtagag gctggggtgt
      241 cggattccat tccttccatt gtccttgtta aatcggccat ccattcaagg tcagattgag
      301 cttgttggta tgagacagga tgtatgtaag tataagcgtc tatgcttaca tggtatagat
      361 gggtttccct ccaggagtgt agatcttcgt ggcagcgaag atctgattct gtgaagggcg
      421 acacatacgg ttcaggttgt ggagggaata atttgttggc tgaatattcc agccattgaa
      481 gctttgttgc ccattcatga gggaattctt ccttgatcat gtcaagatat tcctccttag
      541 acgttgcagt ctggataata gttctccatc gtgcgtcaga tttgcgagga gaaaccttat
      601 gatctcggaa atctcctctg gttttaatat ctccgtcctt tgatatgtaa tcaaggactt
      661 gtttagagtt tctagctggc tggatattag ggtgatttcc ttcaaaatcg aaaaaagaag
      721 gatccctaat acaaggtttt ttatcaagct ggagaagagc atgatagtgg gtagtgccat
      781 cttgatgaag ctcagaagca acaccaagga agaaaataag aaaaggtgtg agtttctccc
      841 agagaaactg gaataaatca tctctttgag atgagcactt gggataggta aggaaaacat
      901 atttagattg gagtctgaag ttcttactag cagaaggcat gttgttgtga ctccgagggg
      961 ttgcctcaaa ctctatctta taaccggcgt ggaggcatgg aggcaggggt attttggtca
     1021 ttttaataga tagtggaaaa tgacgtggaa tttacttaaa gacgaagtct ttgcgacaag
     1081 ggggggccca cgccgaattt aatattaccg gcgtggcccc cccttatcgc gagtgcttta
     1141 gcacgagcgg tccagattta aagtagaaaa tttcccgccc actagggtta aaggtgttca
     1201 cactataaaa gcatatacga tgtgatggta tttgatggag cgtatattgt atcaggtatt
     1261 tccgttggat acgaattatt cgtacgaccc tcatagttta aactatcagt gtttgacagg
     1321 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataacgg atatttaaaa
     1381 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc
     1441 ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac
     1501 gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg
     1561 ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga
     1621 atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc
     1681 tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc
     1741 acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc
     1801 cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc
     1861 tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg
     1921 ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc
     1981 gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc
     2041 gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta
     2101 ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg
     2161 tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc
     2221 gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat
     2281 tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa
     2341 accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat
     2401 gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacggc tcaaccgtgc ggctgcatga
     2461 aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga
     2521 agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca
     2581 tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg
     2641 aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat
     2701 ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag
     2761 ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc
     2821 gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc
     2881 gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg
     2941 ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg
     3001 gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg
     3061 gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg
     3121 cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc
     3181 acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc
     3241 gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca
     3301 caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca
     3361 gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca
     3421 ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat
     3481 acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg
     3541 gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc
     3601 atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc
     3661 aatggcactg gaacccccaa gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc
     3721 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc
     3781 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
     3841 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa
     3901 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
     3961 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
     4021 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
     4081 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
     4141 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
     4201 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
     4261 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
     4321 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
     4381 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
     4441 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
     4501 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
     4561 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
     4621 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
     4681 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
     4741 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
     4801 agcaggggaa aaaggtcgaa aaggcctctt tcctgtggat agcacgtaca ttgggaaccc
     4861 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
     4921 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
     4981 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
     5041 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc
     5101 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc
     5161 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca
     5221 tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     5281 tcccggaaac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     5341 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     5401 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     5461 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     5521 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5581 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5641 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5701 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5761 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5821 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5881 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5941 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6001 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6061 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6121 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6181 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6241 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6301 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6361 gcattctagg tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc
     6421 aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc
     6481 cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc
     6541 aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc
     6601 gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc
     6661 gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc
     6721 gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca
     6781 atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt
     6841 ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat
     6901 gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt
     6961 tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata
     7021 ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt
     7081 agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa
     7141 ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct acagtattta
     7201 aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc
     7261 taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac
     7321 atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat
     7381 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
     7441 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
     7501 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
     7561 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
     7621 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtagagc tcaaagttta
     7681 acgcgttagc agaaggcatg ttgttgtgac tccgaggggt tgcctcaaac tctatcttat
     7741 aaccggcgtg gaggcatgga ggcaggggta ttttggtcat tttaatagat agtggaaaat
     7801 gacgtggaat ttacttaaag acgaagtctt tgcgacaagg gggggcccac gccgaattta
     7861 atattaccgg cgtggccccc ccttatcgcg agtgctttag cacgagcggt ccagatttaa
     7921 agtagaaaat ttcccgccca ctagggttaa aggtgttcac actataaaag catatacgat
     7981 gtgatggtat ttgatggagc gtatattgta tcaggtattt ccgttggata cgaattattc
     8041 gtacgaccct cggtaccgat cggcgcgcca gatttgcctt ttcaatttca gaaagaatgc
     8101 taacccacag atggttagag aggcttacgc agcaggtatc atcaagacga tctacccgag
     8161 caataatctc caggaaatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt
     8221 caggactaac tgcatcaaga acacagagaa agatatattt ctcaagatca gaagtactat
     8281 tccagtatgg acgattcaag gcttgcttca caaaccaagg caagtaatag agattggagt
     8341 ctctaaaaag gtagttccca ctgaatcaaa ggccatggag tcaaagattc aaatagagga
     8401 cctaacagaa ctcgccgtaa agactggcga acagttcata cagagtctct tacgactcaa
     8461 tgacaagaag aaaatcttcg tcaacatggt ggagcacgac acacttgtct actccaaaaa
     8521 tatcaaagat acagtctcag aagaccaaag ggcaattgag acttttcaac aaagggtaat
     8581 atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg tgaagatagt
     8641 ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ccatcgttga
     8701 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     8761 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
     8821 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
     8881 ttcatttcat ttggagagaa cacgggggac tcctgcagga tggaagacgc caaaaacata
     8941 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat
     9001 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
     9061 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg
     9121 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
     9181 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
     9241 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
     9301 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa
     9361 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
     9421 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat
     9481 agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt
     9541 gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt
     9601 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
     9661 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
     9721 tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg
     9781 gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct
     9841 aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt
     9901 gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca
     9961 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
    10021 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga
    10081 ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg
    10141 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
    10201 gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat
    10261 caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgct
    10321 ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
    10381 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
    10441 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
    10501 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
    10561 atcgccgtgt gacgtcgacg atatgaagat gaagatgaaa tatttggtgt gtcaaataaa
    10621 aagcttgtgt gcttaagttt gtgttttttt cttggcttgt tgtgttatga atttgtggct
    10681 ttttctaata ttaaatgaat gtaagatcac attataatga ataaacaaat gtttctataa
    10741 tccattgtga atgttttgtt ggatctcttc tgcagcatat aactactgta tgtgctatgg
    10801 tatggactat ggaatatgat taaagataag ccagagctct ggtgacggac ccatggcttc
    10861 gttgaacaac ggaaactcga cttgccttcc gcacaataca tcatttcttc ttagcttttt
    10921 ttcttcttct tcgttcatac agtttttttt tgtttatcag cttacatttt cttgaaccgt
    10981 agctttcgtt ttcttctttt taactttcca ttcggagttt ttgtatcttg tttcatagtt
    11041 tgtcccagga ttagaatgat taggcatcga accttcaaga atttgattga ataaaacatc
    11101 ttcattctta agatatgaag ataatcttca aaaggcccct gggaatctga aagaagagaa
    11161 gcaggcccat ttatatggga aagaacaata gtatttctta tataggccca tttaagttga
    11221 aaacaatctt caaaagtccc acatcgctta gataagaaaa cgaagctgag tttatataca
    11281 gctagagtcg aagtagtgat tgttggtagt agcgactcca tggttttaga gctagaaata
    11341 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
    11401 tttttcccgg ggcgcgccga tatcgagctc tcccggcgcg ccgatatcga gctcagtgtt
    11461 tgatcgccgg cggtaccgag tgtacttcaa gtcagtggga aatcaataaa atgattattt
    11521 tatgaatata tttcattgtg caagtagata gaaattacat atgttacata acacacgaaa
    11581 taaacaaaaa aagacaatcc aaaaacaaac accccaaaaa aaataatcac tttagataaa
    11641 ctcgtatgag gagaggcacg ttcagtgact cgacgattcc cgagcaaaaa aagtctcccc
    11701 gtcacacatg tagtgggtga cgcaattatc tttaaagtaa tccttctgtt gacttgtcat
    11761 tgataacatc cagtcttcgt caggattgca aagaattata gaagggatcc ca
//
