LOCUS       sequence-243262-       14438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTet expression of Venus, Dual inducible expression of 
            dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14438)
  AUTHORS   Langan RA, Boyken SE, Ng AH, Samson JA, Dods G, Westbrook AM, Nguyen
            TH, Lajoie MJ, Chen Z, Berger S, Mulligan VK, Dueber JE, Novak WRP, 
            El-Samad H, Baker D
  TITLE     De novo design of bioactive protein switches.
  JOURNAL   Nature. 2019 Jul 24. pii: 10.1038/s41586-019-1432-8. doi: 
            10.1038/s41586-019-1432-8.
  PUBMED    31341284
REFERENCE   2  (bases 1 to 14438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2019 Jul 24. pii: 10.1038/s41586-019-1432-8. doi: 
            10.1038/s41586-019-1432-8."
COMMENT     Sequence Label: pAN1646
FEATURES             Location/Qualifiers
     source          1..14438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(117..135,136..158,159..177,178..200,201..219,220..242,
                     243..261,262..284,285..303,304..326,327..345,346..368,
                     369..387)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     CDS             582..1295
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1311..1498
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1366..1390)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1649..2090
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    1649..1766
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     CDS             2117..2137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6254..6274
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6308..6457
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             10221..10241
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10711..11514
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(12156..12971)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(12156..12971)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12879..12898
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     12963..12982
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      13196..13784
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13685..13704
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgatgag gtcgctctta ttgaccacac ctctaccggc
       61 agatccgcta gggataacag ggtaatatag atcaattcct cgatcgagtt taccactccc
      121 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      181 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      241 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      301 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      361 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agctcggtac cctatggcat
      421 gcatgtgctc tgtatgtata taaaactctt gttttcttct tttctctaaa tattctttcc
      481 ttatacatta ggtcctttgt agcataaatt actatacttc tatagacacg caaacacaaa
      541 tacacacact aaattacccg gatcaattcg ggatgagatc tatgtctaaa ggtgaagaat
      601 tattcactgg tgttgtccca attttggttg aattagatgg tgatgttaat ggtcacaaat
      661 tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg taaattgacc ttaaaattga
      721 tttgtactac tggtaaattg ccagttccat ggccaacctt agtcactact ttaggttatg
      781 gtttgcaatg ttttgctaga tacccagatc atatgaaaca acatgacttt ttcaagtctg
      841 ccatgccaga aggttatgtt caagaaagaa ctattttttt caaagatgac ggtaactaca
      901 agaccagagc tgaagtcaag tttgaaggtg ataccttagt taatagaatc gaattaaaag
      961 gtattgattt taaagaaggt ggtaacattt taggtcacaa attggaatac aactataact
     1021 ctcacaatgt ttacatcact gctgacaaac aaaagaatgg tatcaaagct aacttcaaaa
     1081 ttagacacaa cattgaagat ggtggtgttc aattagctga ccattatcaa caaaatactc
     1141 caattggtga tggtccagtc ttgttaccag acaaccatta cttatcctat caatctgcct
     1201 tatccaaaga tccaaacgaa aagagagatc acatggtctt gttagaattt gttactgctg
     1261 ctggtattac ccatggtatg gatgaattgt acaaaggatc ctaactcgag gcgaatttct
     1321 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
     1381 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
     1441 tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca
     1501 tgccgagcaa atgcctgcaa atcgctcccc atttcgctgc caaaccagat gtcaacacag
     1561 ctacaacgcc ccattatctt agcctaaaaa aaccttctct ttggaacttt cagtaatacg
     1621 cttaactgct cattgctata ttgaagtacg gattagaagc cgccgagcgg gcgacagccc
     1681 tccgacggat gactctcctc cgtgcgtcct cgtcatcacc ggtcgcgttc ctgaaacgca
     1741 gatgtgcctc gcgccgcact gctccgaaca ataaagattc tacaatacta gcttttatgg
     1801 ttatgaagag gaaaaattgg cagtaacctg gccccacaaa ccttcaaatt aacgaatcaa
     1861 attaacaacc ataggatgat aatgcgatta gttttttagc cttatttctg gggtaattaa
     1921 tcagcgaagc gatgattttt gatctattaa cagatatata aatggaaaag ctgcataacc
     1981 actttaacta atactttcaa cattttcagt ttgtattact tcttattcaa atgtcataaa
     2041 agtatcaaca aaaaattgtt aatatacctc tatactttaa cgtcaaggag aaaaaactat
     2101 aagatctatg gacgtcccaa agaagaagag aaaggtaggc agcgacaaga agtattctat
     2161 cggactggcc atcgggacta atagcgtcgg gtgggccgtg atcactgacg agtacaaggt
     2221 gccctctaag aagttcaagg tgctcgggaa caccgaccgg cattccatca agaaaaatct
     2281 gatcggagct ctcctctttg attcagggga aactgctgaa gcaacccgcc tcaagcggac
     2341 tgctagacgg cggtacacca ggaggaagaa ccggatttgt taccttcaag agatattctc
     2401 caacgaaatg gcaaaggtcg acgacagctt cttccatagg ctggaagaat cattcctcgt
     2461 ggaagaggat aagaagcatg aacggcatcc catcttcggt aatatcgtcg acgaggtggc
     2521 ctatcacgag aaatacccaa ccatctacca tcttcgcaaa aagctggtgg actcaaccga
     2581 caaggcagac ctccggctta tctacctggc cctggcccac atgatcaagt tcagaggcca
     2641 cttcctgatc gagggcgacc tcaatcctga caatagcgat gtggataaac tgttcatcca
     2701 gctggtgcag acttacaacc agctctttga agagaacccc atcaatgcaa gcggagtcga
     2761 tgccaaggcc attctgtcag cccggctgtc aaagagccgc agacttgaga atcttatcgc
     2821 tcagctgccg ggtgaaaaga aaaatggact gttcgggaac ctgattgctc tttcacttgg
     2881 gctgactccc aatttcaagt ctaatttcga cctggcagag gatgccaagc tgcaactgtc
     2941 caaggacacc tatgatgacg atctcgacaa cctcctggcc cagatcggtg accaatacgc
     3001 cgaccttttc cttgctgcta agaatctttc tgacgccatc ctgctgtctg acattctccg
     3061 cgtgaacact gaaatcacca aggcccctct ttcagcttca atgattaagc ggtatgatga
     3121 gcaccaccag gacctgaccc tgcttaaggc actcgtccgg cagcagcttc cggagaagta
     3181 caaggaaatc ttctttgacc agtcaaagaa tggatacgcc ggctacatcg acggaggtgc
     3241 ctcccaagag gaattttata agtttatcaa acctatcctt gagaagatgg acggcaccga
     3301 agagctcctc gtgaaactga atcgggagga tctgctgcgg aagcagcgca ctttcgacaa
     3361 tgggagcatt ccccaccaga tccatcttgg ggagcttcac gccatccttc ggcgccaaga
     3421 ggacttctac ccctttctta aggacaacag ggagaagatt gagaaaattc tcactttccg
     3481 catcccctac tacgtgggac ccctcgccag aggaaatagc cggtttgctt ggatgaccag
     3541 aaagtcagaa gaaactatca ctccctggaa cttcgaagag gtggtggaca agggagccag
     3601 cgctcagtca ttcatcgaac ggatgactaa cttcgataag aacctcccca atgagaaggt
     3661 cctgccgaaa cattccctgc tctacgagta ctttaccgtg tacaacgagc tgaccaaggt
     3721 gaaatatgtc accgaaggga tgaggaagcc cgcattcctg tcaggcgaac aaaagaaggc
     3781 aattgtggac cttctgttca agaccaatag aaaggtgacc gtgaagcagc tgaaggagga
     3841 ctatttcaag aaaattgaat gcttcgactc tgtggagatt agcggggtcg aagatcggtt
     3901 caacgcaagc ctgggtacct accatgatct gcttaagatc atcaaggaca aggattttct
     3961 ggacaatgag gagaaagagg acatccttga ggacattgtc ctgactctca ctctgttcga
     4021 ggaccgggaa atgatcgagg agaggcttaa gacctacgcc catctgttcg acgataaagt
     4081 gatgaagcaa cttaaacgga gaagatatac cggatgggga cgccttagcc gcaaactcat
     4141 caacggaatc cgggacaaac agagcggaaa gaccattctt gatttcctta agagcgacgg
     4201 attcgctaat cgcaacttca tgcaacttat ccatgatgat tccctgacct ttaaggagga
     4261 catccagaag gcccaagtgt ctggacaagg tgactcactg cacgagcata tcgcaaatct
     4321 ggctggttca cccgctatta agaagggtat tctccagacc gtgaaagtcg tggacgagct
     4381 ggtcaaggtg atgggtcgcc ataaaccaga gaacattgtc atcgagatgg ccagggaaaa
     4441 ccagactacc cagaagggac agaagaacag cagggagcgg atgaaaagaa ttgaggaagg
     4501 gattaaggag ctcgggtcac agatccttaa agagcacccg gtggaaaaca cccagcttca
     4561 gaatgagaag ctctatctgt actaccttca aaatggacgc gatatgtatg tggaccaaga
     4621 gcttgatatc aacaggctct cagactacga cgtggacgcc atcgtccctc agagcttcct
     4681 caaagacgac tcaattgaca ataaggtgct gactcgctca gacaagaacc ggggaaagtc
     4741 agataacgtg ccctcagagg aagtcgtgaa aaagatgaag aactattggc gccagcttct
     4801 gaacgcaaag ctgatcactc agcggaagtt cgacaatctc actaaggctg agaggggcgg
     4861 actgagcgaa ctggacaaag caggattcat taaacggcaa cttgtggaga ctcggcagat
     4921 tactaaacat gtagcccaaa tccttgactc acgcatgaat accaagtacg acgaaaacga
     4981 caaacttatc cgcgaggtga aggtgattac cctgaagtcc aagctggtca gcgatttcag
     5041 aaaggacttt caattctaca aagtgcggga gatcaataac tatcatcatg ctcatgacgc
     5101 atatctgaat gccgtggtgg gaaccgccct gatcaagaag tacccaaagc tggaaagcga
     5161 gttcgtgtac ggagactaca aggtctacga cgtgcgcaag atgattgcca aatctgagca
     5221 ggagatcgga aaggccaccg caaagtactt cttctacagc aacatcatga atttcttcaa
     5281 gaccgaaatc acccttgcaa acggtgagat ccggaagagg ccgctcatcg agactaatgg
     5341 ggagactggc gaaatcgtgt gggacaaggg cagagatttc gctaccgtgc gcaaagtgct
     5401 ttctatgcct caagtgaaca tcgtgaagaa aaccgaggtg caaaccggag gcttttctaa
     5461 ggaatcaatc ctccccaagc gcaactccga caagctcatt gcaaggaaga aggattggga
     5521 ccctaagaag tacggcggat tcgattcacc aactgtggct tattctgtcc tggtcgtggc
     5581 taaggtggaa aaaggaaagt ctaagaagct caagagcgtg aaggaactgc tgggtatcac
     5641 cattatggag cgcagctcct tcgagaagaa cccaattgac tttctcgaag ccaaaggtta
     5701 caaggaagtc aagaaggacc ttatcatcaa gctcccaaag tatagcctgt tcgaactgga
     5761 gaatgggcgg aagcggatgc tcgcctccgc tggcgaactt cagaagggta atgagctggc
     5821 tctcccctcc aagtacgtga atttcctcta ccttgcaagc cattacgaga agctgaaggg
     5881 gagccccgag gacaacgagc aaaagcaact gtttgtggag cagcataagc attatctgga
     5941 cgagatcatt gagcagattt ccgagttttc taaacgcgtc attctcgctg atgccaacct
     6001 cgataaagtc cttagcgcat acaataagca cagagacaaa ccaattcggg agcaggctga
     6061 gaatatcatc cacctgttca ccctcaccaa tcttggtgcc cctgccgcat tcaagtactt
     6121 cgacaccacc atcgaccgga aacgctatac ctccaccaaa gaagtgctgg acgccaccct
     6181 catccaccag agcatcaccg gactttacga aactcggatt gacctctcac agctcggagg
     6241 ggatgaggga gctcccaaga aaaagcgcaa ggtaggtagt tccgaggcca gcggttccgg
     6301 acgggctgac gcattggacg attttgatct ggatatgctg ggaagtgacg ccctcgatga
     6361 ttttgacctt gacatgcttg gttcggatgc cctcgatgac ttcgatctgg acatgctggg
     6421 atctgacgct cttgatgatt ttgaccttga catgctgatt aactctagag gttctggatc
     6481 tgggtcgggt tcaggctctg gatcggtatc aaaaggcgag gcggtcatta aagagttcat
     6541 gaggtttaaa gtccatatgg aaggaagcat gaatggtcac gagtttgaga tagagggaga
     6601 gggtgaggga aggccgtacg aagggacgca aactgcgaag ttgaaggtaa cgaaaggcgg
     6661 ccctctgcca ttctcttggg acatcctttc accgcaattt atgtacggct ctagggcctt
     6721 cacaaaacac cccgccgata ttccggatta ctataaacaa agttttcctg agggcttcaa
     6781 gtgggaaaga gtaatgaact tcgaggatgg gggagctgtg acggttactc aagacactag
     6841 cctggaggac ggtactctta tatacaaggt aaaactaaga gggacgaatt ttccgccaga
     6901 cggccccgtg atgcaaaaaa aaactatggg gtgggaagca tccaccgaga ggctgtaccc
     6961 cgaagacggg gtcctgaagg gtgatattaa aatggcatta agattaaaag atggcgggag
     7021 atacttagct gacttcaaga ctacatataa agccaaaaaa cctgtacaga tgcctggagc
     7081 ttacaatgtg gatcgtaagc tggatattac atcccacaac gaagattata ccgtggtgga
     7141 gcagtacgag cgttcagagg ggcgtcactc caccgggggt atggatgaat tatacaagag
     7201 cggtcttaga agcagggcgc aagcgtcaaa ttcagcggta gatggaacag caggtccagg
     7261 tagtactggc agcagaggat ctgggtcggg ttcaggctct ggatcgggat ccagtaagga
     7321 agcagtgacg aagctacagg cccttaacat caaattggcg gaaaaattgc ttgaagctgt
     7381 cacgaagttg caagcattaa acataaaact tgccgaaaag ctgttagaag ccctggcacg
     7441 tctgcaagag ctgaacattg ccttggttta tttagccgta gaattaacgg accctaagag
     7501 aattgcggac gagattaaaa aagttaaaga caagagcaag gaaattgtcg agagagcgga
     7561 agaggagata gctagggcgg cagccgagtc caagaaaatt ttagacgaag ccgaggagga
     7621 aatcgcccgt gcagcagcag agtccaagaa gatccttgat gaaggatccg gaagtggttc
     7681 agacgcggta gcggaattac aggcattaaa tctgaagcta gccgagttac ttctggaggc
     7741 tgtggctgag cttcaagcgc ttaacctgaa actagcggag ctacttttgg aggctatagc
     7801 taagctacaa gagctgaata ttaagcttgt cgagctattg actaaactaa ctgaccctgc
     7861 taccatcagg gaggcgatca gaaaggtgaa ggaagactcc gagaggatag tggcggaggc
     7921 agagcgtttg atagccgctg cgaaggctga aagtgaacgt attatacgtg aagctgagag
     7981 attgatagct gctgctaaag cggagtccga gaggataatt agagaggggt ccggttcagg
     8041 ggaccctgac gtagcgagac tgcaagaact aaatatcgag ctagctagag agctgctaag
     8101 ggatgtcgct agactacaag agctaaacat agaactggcg agagaacttc taagggcagc
     8161 ggcagagtta caagagctga acataaagtt agtcgagcta gcgagtgagt taaccgatcc
     8221 tgatgaagca cgtaaagcaa tcgccagggt aaagagagag agtaaaagaa tcgttgagga
     8281 tcttattatg agctgtgccc aagagtctgc tgcaagcgag aagattagca gagaagccga
     8341 aagactgata aggtaatggc attgaattga attgaaatcg atagatcaat ttttttcttt
     8401 tctctttccc catcctttac gctaaaataa tagtttattt tattttttga atatttttta
     8461 tttatatacg tatatataga ctattattta tcttttaatg attattaaga tttttattaa
     8521 aaaaaaattc gctcctcttt taatgccttt atgcagtttt tttttcccat tcgatatttc
     8581 tatgtgctgg atgcacacac tggcttaaga tgacaacgtt atattgaatt ttcaaaaatt
     8641 cttacttttt ttttggatgg acgcaaagaa gtttaataat catattacat ggcattacca
     8701 ccatatacat atccatatac atatccatat ctaatcttac ttatatgttg tggaaatgta
     8761 aagagcccca ttatcttagc ctaaaaaaac cttctctttg gaactttcag taatacgctt
     8821 aactgctcat tgctatattg aagtgtggcc gcgtgggcgt gcgtgggcgg gcgtgggcgt
     8881 gcgtgggcgg gcgtgggcgt gcgtgggcgt ctagaccgtg cgtcctcgtc ttcaccggtc
     8941 gcgttcctga aacgcagatg tgcctaatgc cgcactgctc cgaacaataa agattctaca
     9001 atactagctt ttatggttat gaagaggaaa aattggcagt aacctggccc cacaaacctt
     9061 caaattaacg aatcaaatta acaaccatag gatgataatg cgattagttt tttagcctta
     9121 tttctggggt aattaatcag cgaagcgatg atttttgatc tattaacaga tatataaatg
     9181 gaaaagctgc ataaccactt taactaatac tttcaacatt ttcagtttgt attacttctt
     9241 attcaaatgt cataaaagta tcaacaaaaa attgttaata tacctctata ctttaacgtc
     9301 aaggagaaaa aactataaga tctatggagg ctagaaaagc catcgctaga gtaaagagag
     9361 aatcaaagag aatcgtagaa gatgctgaga ggttaataag agaagcagcc gccgcatctg
     9421 agaaaatttc aagggaggcc gaaaggttga ttagggaggc agctgcggct tcagaaaaga
     9481 taagtaggga gggttctgtt tcaaagggtg aagaattgat caaagaaaac atgcatatga
     9541 aattatatat ggaaggtaca gttgacaacc atcattttaa gtgtacatct gaaggagaag
     9601 gtaagcctta cgagggaact caaactatga gaataaaagt cgtagaaggt ggaccattgc
     9661 cttttgcttt tgatatctta gcaacatctt tcttatatgg ttctaaaacc tttattaacc
     9721 atactcaagg tatccctgat ttcttcaaac aatctttccc tgaaggattt acttgggaga
     9781 gggttacaac atatgaggat ggtggtgttt tgacagcaac ccaggataca tcattgcagg
     9841 atggatgttt gatttataat gtaaagatta gaggagtaaa ttttacttct aacggtcctg
     9901 taatgcaaaa gaagacttta ggttgggaag ctttcaccga aacattatat ccagcagacg
     9961 gaggtttaga gggaagaaac gatatggctt tgaagttagt cggtggttca cacttaatag
    10021 ctaacgccaa gactacttac aggtctaaga aaccagcaaa gaatttgaaa atgccaggag
    10081 tttattatgt tgactacaga ttggaaagga tcaaggaggc caataacgag acatatgtag
    10141 agcagcatga agtcgccgtc gccagatatt gcgacttacc ttctaagttg ggtcataaat
    10201 tgaatggatc cggttctcca ccaaagaaga agagaaaggt tggttcttaa ctcgagtggc
    10261 gccaattggt gcggcaattg ataataacga aaatgtcttt taatgatctg ggtataatga
    10321 ggaattttcc gaacgttttt actttatata tatatataca tgtaacatat attctatacg
    10381 ctatagagaa aggaaatttt tcaattaaaa aaaaaataga gaaagagttt cacttcttga
    10441 ttatcgctaa cactaatggt tgaagtactg ctactttaat tttatgctga gcagttacag
    10501 agatgttacg aaccactagt gcactgcagt acacggtttc cttgaaattt ttttgattcg
    10561 gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata
    10621 cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga
    10681 acaaaaacgt gcaggaaacg aagataaatc atgtcgaaag ctacatataa ggaacgtgct
    10741 gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga aaagcaaaca
    10801 aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt agttgaagca
    10861 ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga tttttccatg
    10921 gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt actcttcgaa
    10981 gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc gggtgtatac
    11041 agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc aggtattgtt
    11101 agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct tttgatgtta
    11161 gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg tactgttgac
    11221 attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga catgggtgga
    11281 agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga tgacaaggga
    11341 gatgcattgg gtcaacagta tagaaccgtg gatgatgtgg tttctacagg atctgacatt
    11401 attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga gggtgaacgt
    11461 tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa ctaaaaaact
    11521 gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat ttaattatat
    11581 cagttattac ccgagtagag cacttgaatc cactgccccg ggaatctcgg tcgtaatgat
    11641 ttctataatg acgaaaaaaa aaaaattgga aagaaaaagc ttcatggcct ttataaaaag
    11701 gaactatcca atacctcgcc agaaccaagt aacagtattt tacggggcac aaatcaagaa
    11761 caataagaca ggactgtaaa gatggacgca ttgaactcca aagaacaaca agagttccaa
    11821 aaagtagtgg aacaaaagca aatgaaggat ttcatgcgtt tgtactctaa tctggtagaa
    11881 agatgtttca cagactgtgt caatgacttc acaacatcaa agctaaccaa taaggaacaa
    11941 acatgcatca tgaagtgctc agaaaagttc ttgaagcata gcgaacgtgt agggcagcgt
    12001 ttccaagaac aaaacgctgc cttgggacaa ggcttgggcc gataaggtgt actggcgtat
    12061 atatatctaa ttatgtatct ctggtgtagc ccatttttag catgtaaata taaagaccga
    12121 gcggccgcgt gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa
    12181 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
    12241 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
    12301 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
    12361 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc
    12421 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
    12481 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgaggcg aaatacgcga
    12541 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
    12601 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
    12661 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
    12721 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
    12781 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
    12841 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
    12901 tataaatcag catccatgtt ggaatttaat cgcggcctgg agcaagacgt ttcccgttga
    12961 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
    13021 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
    13081 tgttgaataa atcgaacttt tgctgagttg aaggatcagt catgaccaaa atcccttaac
    13141 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    13201 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    13261 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    13321 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    13381 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    13441 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    13501 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    13561 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    13621 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    13681 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    13741 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    13801 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    13861 cccctgattc tgtggataac cgtgcggccg ccaatgctaa attcgagtga aacacaggaa
    13921 gatcagaaaa tcctcatttc atccatatta acaataattt caaatgttta tttgcattat
    13981 ttgaaactag gcaagacaag caacgaaacg tttttgaaaa ttttgagtat tttcaataaa
    14041 tttgtagagg actcagatat tgaaaaaaag ctacagcaat taatacttga taagaagagt
    14101 attgagaagg gcaacggttc atcatctcat ggatctgcac atgaacaaac accagagtca
    14161 aacgacgttg aaattgaggc tactgcgcca attgatgaca atacagacga tgataacaaa
    14221 ccgaagttat ctgatgtaga aaaggattaa agatgctaag agatagtgat gatatttcat
    14281 aaataatgta attctatata tgttaattac cttttttgcg aggcatattt atggtgaagg
    14341 ataagttttg accatcaaag aaggttaatg tggctgtggt ttcagggtcc ataaagccca
    14401 catggataac attacccctg aattcgcatc tagactga
//
