LOCUS       sequence-243266-       10025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Progesterone inducible expression of KeyC-BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10025)
  AUTHORS   Langan RA, Boyken SE, Ng AH, Samson JA, Dods G, Westbrook AM, Nguyen
            TH, Lajoie MJ, Chen Z, Berger S, Mulligan VK, Dueber JE, Novak WRP, 
            El-Samad H, Baker D
  TITLE     De novo design of bioactive protein switches.
  JOURNAL   Nature. 2019 Jul 24. pii: 10.1038/s41586-019-1432-8. doi: 
            10.1038/s41586-019-1432-8.
  PUBMED    31341284
REFERENCE   2  (bases 1 to 10025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2019 Jul 24. pii: 10.1038/s41586-019-1432-8. doi: 
            10.1038/s41586-019-1432-8."
COMMENT     Sequence Label: pAN1579
FEATURES             Location/Qualifiers
     source          1..10025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3750..3773
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     promoter        5210..5617
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             5618..6712
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(7743..8558)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7743..8558)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8466..8485
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8550..8569
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8783..9371
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9272..9291
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgaaggc aagcccagaa aaatatcgca agcacctttg
       61 gtcttacagt gccaactttt ggcctgccga cgttaagagt acaaagctga tggcaatgta
      121 cgacaagata acagagtctc aaaagaagtg aaacaatttt tcttcaccac attttccatt
      181 gttccttccc cccataacta taaacgtatt tatgtatata tatttgcgtg taagtgtgtg
      241 tactataggg caccgtaaag taataatgct taattagtta ctactatgac catataagag
      301 gtcatactgt atgaagccac aaagcagata gatcaatcat gtttaacgaa aactgttaat
      361 cgaagattat ttcttttttt ttttctcttt cctttttaca aagaaaattt tttttgcgct
      421 ttttgccatc accatcgcaa gttctgggac aattgttctc tttcgctcca gttccaagga
      481 aagaggtttc tgttttactt aatagaaagt gtcatcttgt attttatatc tcttctttct
      541 tgtgtaaaat tctttagttt tgattttgta tttttaggac agtgagctac gaagtaacat
      601 ttttacttaa taaccgtttg aagcatagag caggccctgg tatcaccacc taatatctgg
      661 ctttttattc aataaaaact caaaaaaaaa aatccaaaaa aaactaaaaa accaataaaa
      721 ataaaagatc tatgggtacc cgcccatatg cttgccctgt cgagtcctgc gatcgccgct
      781 tttctcgctc ggatgagctt acccgccata tccgcatcca taccggtcag aagcccttcc
      841 agtgtcgaat ctgcatgcgt aacttcagtc gtagtgacca ccttaccacc cacatccgca
      901 cccacacagg cgagaagcct tttgcctgtg acatttgtgg gaggaagttt gccaggagtg
      961 atgaacgcaa gaggcatacc aaaatccata caggtagatc cggtgacggt gctggtttaa
     1021 agttcaataa agtcagagtt gtgagagcac tggatgctgt tgctctccca cagccattgg
     1081 gcgttccaaa tgaaagccaa gccctaagcc agagattcac tttttcacca ggtcaagaca
     1141 tacagttgat tccaccactg atcaacctgt taatgagcat tgaaccagat gtgatctatg
     1201 caggacatga caacacaaaa cctgacacct ccagttcttt gctgacaagt cttaatcaac
     1261 taggcgagag gcaacttctt tcagtagtca agtggtctaa atcattgcca ggttttcgaa
     1321 acttacatat tgatgaccag ataactctca ttcagtattc ttggatgagc ttaatggtgt
     1381 ttggtctagg atggagatcc tacaaacatg tcagtgggca gatgctgtat tttgcacctg
     1441 atctaatact aaatgaacag cggatgaaag aatcatcatt ctattcatta tgccttacca
     1501 tgtggcagat cccacaggag tttgtcaagc ttcaagttag ccaagaagag ttcctctgta
     1561 tgaaagtatt gttacttctt aatacaattc ctttggaagg gctacgaagt caaacccagt
     1621 ttgaggagat gaggtcaagc tacattagag agctcatcaa ggcaattggt ttgaggcaaa
     1681 aaggagttgt gtcgagctca cagcgtttct atcaacttac aaaacttctt gataactcgc
     1741 atgatcttgt caaacagctt catctgtact gcttgaatac atttatccag tcccgggcac
     1801 tgagtgttga atttccagaa atgatgtctg aagttattgc tgcacaatta cccaagatat
     1861 tggcagggat ggtgaaaccc cttctctttc ataaaaaggc ggctgcaggt gacggtgctg
     1921 gtttaattaa catgacggtc gaccatgatt tcaatagcga agatatttta ttccccatag
     1981 aaagcatgag tagtatacaa tacgtggaga ataataaccc aaataatatt aacaacgatg
     2041 ttatcccgta ttctctagat atcaaaaaca ctgtcttaga tagtgcggat ctcaatgaca
     2101 ttcaaaatca agaaacttca ctgaatttgg ggcttcctcc actatctttc gactctccac
     2161 tgcccgtaac ggaaacgata ccatccacta ccgataacag cttgcatttg aaagctgata
     2221 gcaacaaaaa tcgcgatgca agaactattg aaaatgatag tgaaattaag agtactaata
     2281 atgctagtgg ctctggggca aatcaataca caactcttac ttcaccttat cctatgaacg
     2341 acattttgta caacatgaac aatccgttac aatcaccgtc accttcatcg gtacctcaaa
     2401 atccgactat aaatcctccc ataaatacag caagtaacga aactaattta tcgcctcaaa
     2461 cttcaaatgg taatgaaact cttatatctc ctcgagccca acaacatacg tccattaaag
     2521 ataatcgtct gtccttacct aatggtgcta attcgaatct tttcattgac actaacccaa
     2581 acaatttgaa cgaaaaacta agaaatcaat tgaactcaga tacaaattca tattctaact
     2641 ccatttctaa ttcaaactcc aattctacgg gtaatttaaa ttccagttat tttaattcac
     2701 tgaacataga ctccatgcta gatgattacg tttctagtga tctcttattg aatgatgatg
     2761 atgatgacac taatttatca cgccgaagat ttagcgacgt tataacaaac caagagctcg
     2821 gatcctaact cgagataaag caatcttgat gaggataatg attttttttt gaatatacat
     2881 aaatactacc gtttttctgc tagattttgt gatgacgtaa ataagtacat attacttttt
     2941 aagccaagac aagattaagc attaacttta cccttttctt tctaagtttc aatattagtt
     3001 atcactgttt aaaagttatg gcgagaacgt cggcggttaa aatatattac cctgaacggc
     3061 tgccaaacca gatgtcaaca cagctacaac gttatattga attttcaaaa attcttactt
     3121 tttttttgga tggacgcaaa gaagtttaat aatcatatta catggcatta ccaccatata
     3181 catatccata tacatatcca tatctaatct tacttatatg ttgtggaaat gtaaagagcc
     3241 ccattatctt agcctaaaaa aaccttctct ttggaacttt cagtaatacg cttaactgct
     3301 cattgctata ttgaagtgtg gccgcgtggg cgtgcgtggg cgggcgtggg cgtgcgtggg
     3361 cgggcgtggg cgtgcgtggg cgtctagacc gtgcgtcctc gtcttcaccg gtcgcgttcc
     3421 tgaaacgcag atgtgcctaa tgccgcactg ctccgaacaa taaagattct acaatactag
     3481 cttttatggt tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatta
     3541 acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg
     3601 ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa atggaaaagc
     3661 tgcataacca ctttaactaa tactttcaac attttcagtt tgtattactt cttattcaaa
     3721 tgtcataaaa gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga
     3781 aaaaactata agatctatgt ccagcgaaaa agttagaaga gagttaaaag agtccctaaa
     3841 ggagaaccac aaacagaatc aaaaattgtt gaaagatcac aaacgtgcgc aggagaagct
     3901 aaatagagaa ctggaggagc taaagaaaaa gcataagaaa accttagacg acatccgtag
     3961 ggagtccggt tctgtttcaa agggtgaaga attgatcaaa gaaaacatgc atatgaaatt
     4021 atatatggaa ggtacagttg acaaccatca ttttaagtgt acatctgaag gagaaggtaa
     4081 gccttacgag ggaactcaaa ctatgagaat aaaagtcgta gaaggtggac cattgccttt
     4141 tgcttttgat atcttagcaa catctttctt atatggttct aaaaccttta ttaaccatac
     4201 tcaaggtatc cctgatttct tcaaacaatc tttccctgaa ggatttactt gggagagggt
     4261 tacaacatat gaggatggtg gtgttttgac agcaacccag gatacatcat tgcaggatgg
     4321 atgtttgatt tataatgtaa agattagagg agtaaatttt acttctaacg gtcctgtaat
     4381 gcaaaagaag actttaggtt gggaagcttt caccgaaaca ttatatccag cagacggagg
     4441 tttagaggga agaaacgata tggctttgaa gttagtcggt ggttcacact taatagctaa
     4501 cgccaagact acttacaggt ctaagaaacc agcaaagaat ttgaaaatgc caggagttta
     4561 ttatgttgac tacagattgg aaaggatcaa ggaggccaat aacgagacat atgtagagca
     4621 gcatgaagtc gccgtcgcca gatattgcga cttaccttct aagttgggtc ataaattgaa
     4681 tggatcctaa ctcgagagct tttgattaag ccttctagtc caaaaaacac gtttttttgt
     4741 catttatttc attttcttag aatagtttag tttattcatt ttatagtcac gaatgtttta
     4801 tgattctata tagggttgca aacaagcatt tttcatttta tgttaaaaca atttcaggtt
     4861 taccttttat tctgcttgtg gtgacgcgtg tatccgcccg ctcttttggt cacccatgta
     4921 tgctgagcag ttacagagat gttacgaacc actagtgcac tgcagtacat cgaggagaac
     4981 ttctagtata tctacatacc taatattatt gccttattaa aaatggaatc ccaacaatta
     5041 catcaaaatc cacattctct tcaaaatcaa ttgtcctgta cttccttgtt catgtgtgtt
     5101 caaaaacgtt atatttatag gataattata ctctatttct caacaagtaa ttggttgttt
     5161 ggccgagcgg tctaaggcgc ctgattcaag aaatatcttg accgcagtta actgtgggaa
     5221 tactcaggta tcgtaagatg caagagttcg aatctcttag caaccattat ttttttcctc
     5281 aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg actggaaatt
     5341 ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa aattgggaga
     5401 aaaaggaaag gtgagagcgc cggaaccggc ttttcatata gaatagagaa gcgttcatga
     5461 ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc tattgttttt
     5521 tccaataggt ggttagcaat cgtcttactt tctaactttt cttacctttt acatttcagc
     5581 aatatatata tatatatttc aaggatatac cattctaatg tctgccccta agaagatcgt
     5641 cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc gaagccatta aggttcttaa
     5701 agctatttct gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt taattggtgg
     5761 tgctgctatc gatgctacag gtgttccact tccagatgag gcgctggaag cctccaagaa
     5821 ggctgatgcc gttttgttag gtgctgtggg tggtcctaaa tggggaaccg gtagtgttag
     5881 acctgaacaa ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca acttaagacc
     5941 atgtaacttt gcatccgact ctcttttaga cttatctcca atcaagccac aatttgctaa
     6001 aggtactgac ttcgttgttg tcagagaatt agtgggaggt atttactttg gtaagagaaa
     6061 ggaagacgat ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc cagaagtgca
     6121 aagaatcaca agaatggccg ctttcatggc cctacaacat gagccaccat tgcctatttg
     6181 gtccttggat aaagctaatg ttttggcctc ttcaagatta tggagaaaaa ctgtggagga
     6241 aaccatcaag aacgaatttc ctacattgaa ggttcaacat caattgattg attctgccgc
     6301 catgatccta gttaagaacc caacccacct aaatggtatt ataatcacca gcaacatgtt
     6361 tggtgatatc atctccgatg aagcctccgt tatcccaggt tccttgggtt tgttgccatc
     6421 tgcgtccttg gcctctttgc cagacaagaa caccgcattt ggtttgtacg aaccatgcca
     6481 cggttctgct ccagatttgc caaagaataa ggtcaaccct atcgccacta tcttgtctgc
     6541 tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca ttgaagatgc
     6601 agttaaaaag gttttggatg caggtatcag aactggtgat ttaggtggtt ccaacagtac
     6661 caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa atccttgctt aaaaagattc
     6721 tcttttttta tgatatttgt acataaactt tataaatgaa attcataata gaaacgacac
     6781 gaaattacaa aatggaatat gttcataggg tagacgaaac tatatacgca atctacatac
     6841 atttatcaag aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa attgatacta
     6901 atggctcaac gtgataagga aaaagaattg cactttaaca ttaatattga caaggaggag
     6961 ggcaccacac aaaaagttag gtgtaacaga aaatcatgaa actatgattc ctaatttata
     7021 tattggagga ttttctctaa aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct
     7081 gaccatttga tggagtttaa gtcaatacct tcttgaacca tttcccataa tggtgaaagt
     7141 tccctcaaga attttactct gtcagaaacg gccttaacga cgtagtcgag agtactcgta
     7201 tcgcatgtcg gtgcgacacg aaattacaaa atggaatatg ttcatagggt agacgaaact
     7261 atatacgcaa tctacataca tttatcaaga aggagaaaaa ggaggatgta aaggaataca
     7321 ggtaagcaaa ttgatactaa tggctcaacg tgataaggaa aaagaattgc actttaacat
     7381 taatattgac aaggaggagg gcaccacaca aaaagttagg tgtaacagaa aatcatgaaa
     7441 ctatgattcc taatttatat attggaggat tttctctaaa aaaaaaaaaa tacaacaaat
     7501 aaaaaacact caatgacctg accatttgat ggagtttaag tcaatacctt cttgaaccat
     7561 ttcccataat ggtgaaagtt ccctcaagaa ttttactctg tcagaaacgg ccttaacgac
     7621 gtagtcgacc tcctcttcag tactaaatct accaatacca aatctgatgg aagaatgggc
     7681 taatgcatca tccttaccca gcgccgagcg gccgcgtgtt acaaccaatt aaccaattct
     7741 gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
     7801 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
     7861 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
     7921 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
     7981 actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc
     8041 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
     8101 tgcgcctgag cgaggcgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
     8161 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
     8221 tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca
     8281 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
     8341 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     8401 aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg
     8461 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
     8521 ggcctggagc aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt
     8581 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat
     8641 cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag
     8701 gatcagtcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8761 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8821 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8881 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8941 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9001 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9061 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9121 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9181 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9241 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9301 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9361 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9421 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt gcggccgcca
     9481 atacccagaa gaacagtaaa ataaagcaag gtacgtgaaa ttaatatttt taaatggttc
     9541 taaccgatgc cgaagaactg cgcagtccgg ttataacgtc tgacatgtcc ttttttgatt
     9601 tggaatccaa ccactcaagt gactctgttc atttactttg cgaaaaatat acccacaaat
     9661 tgcccatcga aagtgaatcg caaaccacct tcagactggc accgacaaag caaagattat
     9721 acagacagag tactttatac gtaccgttaa gtctcaagca aagggttttc ttatttactg
     9781 aacgggtaaa gagtatctgg gccggcttgc caagatgcaa accgaataag tatttcaaag
     9841 ttgcatttgc cttagccgtc ctgacaccat tggctatttg gatattttat attgactttc
     9901 gtgtacattg atcacatcga ctgttctatt ggcaaatgaa ccacgggcat tgactatttt
     9961 tcaggttact actatatatt atcatcacgg gcaaggattg taccctgaat tcgcatctag
    10021 actga
//
