LOCUS       sequence-243399-       13414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13414)
  AUTHORS   Zhao X, Sternsdorf T, Bolger TA, Evans RM, Yao TP
  TITLE     Regulation of MEF2 by histone deacetylase 4- and SIRT1 
            deacetylase-mediated lysine modifications.
  JOURNAL   Mol Cell Biol. 2005 Oct;25(19):8456-64.
  PUBMED    16166628
REFERENCE   2  (bases 1 to 13414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-10"; volume: "25(19)"; pages: "8456-64"
COMMENT     Sequence Label: pcDNA3-beta-FLAG-CBP-LD-HA
FEATURES             Location/Qualifiers
     source          1..13414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        108..487
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        488..691
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     642..662
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     736..755
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        736..754
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          791..1363
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             1409..1432
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      6299..6308
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8774..8800
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(8840..8858)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8841..8858)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8878..8895)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8884..9108
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9154..9582
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9241..9260)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9451..9472
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9591..9611)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9596..9925
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9776..9911
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9838..9857
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9992..10786
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10046..10065)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10656..10675
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10960..11081
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10997..11016)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11051..11070
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11130..11146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11130..11146)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11143..11165)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11154..11170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11178..11184,11185..11202,11203..11208))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11223..11244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11361..11378)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11532..12120)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11612..11631)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12291..13082,13083..13151))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12914..12933
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13152..13256)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13331..13350)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
       61 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
      121 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      181 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      241 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      301 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      361 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      421 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      481 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      541 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      601 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      661 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
      721 ctggcttatc gaaattaata cgactcacta tagggagacc caagctagag tcgatcctga
      781 gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt
      841 catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt
      901 gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc
      961 attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca
     1021 tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta
     1081 atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac agttttagag
     1141 aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg
     1201 tggaaatatt cttattggta gaaacaacta catcctggtc atcatcctgc ctttctcttt
     1261 atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg
     1321 cccctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     1381 ggtttgtgct gtcgacccca agcttatgga ctacaaggac gacgacgaca aagccgagaa
     1441 cttgctggac ggaccgccca accccaaacg agccaaactc agctcgcccg gcttctccgc
     1501 gaatgacaac acagattttg gatcattgtt tgacttggaa aatgaccttc ctgatgagct
     1561 gatccccaat ggagaattaa gccttttaaa cagtgggaac cttgttccag atgctgcgtc
     1621 caaacataaa caactgtcag agcttcttag aggaggcagc ggctctagca tcaacccagg
     1681 gataggcaat gtgagtgcca gcagccctgt gcaacagggc cttggtggcc aggctcaggg
     1741 gcagccgaac agtacaaaca tggccagctt aggtgccatg ggcaagagcc ctctgaacca
     1801 aggagactca tcaacaccca acctgcccaa acaggcagcc agcacctctg ggcccactcc
     1861 ccctgcctcc caagcactga atccacaagc acaaaagcaa gtagggctgg tgaccagtag
     1921 tcctgccaca tcacagactg gacctgggat ctgcatgaat gctaacttca accagaccca
     1981 cccaggcctt ctcaatagta actctggcca tagcttaatg aatcaggctc aacaagggca
     2041 agctcaagtc atgaatggat ctcttggggc tgctggaaga ggaaggggag ctggaatgcc
     2101 ctaccctgct ccagccatgc agggggccac aagcagtgtg ctggcggaga ccttgacaca
     2161 ggtttcccca caaatggctg gccatgctgg actaaataca gcacaggcag gaggcatgac
     2221 caagatggga atgactggta ccacaagtcc atttggacaa ccctttagtc aaactggagg
     2281 gcagcagatg ggagccactg gagttaaccc ccagttagcc agcaaacaga gcatggtcaa
     2341 tagtttacct gcttttccta cagatatcaa gaatacttca gtcaccactg tgccaaatat
     2401 gtcccagttg caaacatcag tgggaattgt acccacacaa gcaattgcaa caggccccac
     2461 agcagaccct gaaaaacgca aactgataca gcagcagctg gttctactgc ttcatgccca
     2521 caaatgtcag agacgagagc aagcaaatgg agaggttcga gcctgttctc tcccacactg
     2581 tcgaaccatg aaaaacgttt tgaatcacat gacacattgt caggctggga aagcctgcca
     2641 agttgcccat tgtgcatctt cacgacaaat catctctcat tggaagaact gcacacgaca
     2701 tgactgtcct gtttgcctcc ctttgaaaaa tgccagtgac aagcgaaacc aacaaaccat
     2761 cctgggatct ccagctagtg gaattcaaaa cacaattggt tctgttggtg cagggcaaca
     2821 gaatgccact tccttaagta acccaaatcc catagacccc agttccatgc agcgggccta
     2881 tgctgctcta ggactcccct acatgaacca gcctcagacg cagctgcagc ctcaggttcc
     2941 tggccagcaa ccagcacagc ctccagccca ccagcagatg aggactctca atgccctagg
     3001 aaacaacccc atgagtatcc cagcaggagg aataacaaca gatcaacagc caccaaactt
     3061 gatttcagaa tcagctcttc caacttcctt gggggctacc aatccactga tgaatgatgg
     3121 ttcaaactct ggtaacattg gaagcctcag cacgatacct acagcagcgc ctccttccag
     3181 cactggtgtt cgaaaaggct ggcatgaaca tgtgactcag gacctacgga gtcatctagt
     3241 ccataaactc gttcaagcca tcttcccaac tccagaccct gcagctctga aagatcgccg
     3301 catggagaac ctggttgcct atgctaagaa agtggaggga gacatgtatg agtctgctaa
     3361 tagcagggat gaatactatc atttattagc agagaaaatc tataaaatac aaaaagaact
     3421 agaagaaaag cggaggtcac gtttacataa gcaaggcatc ctgggtaacc agccagcttt
     3481 accagcttct ggggctcagc cccctgtgat tccaccagcc cagtctgtaa gacctccaaa
     3541 tgggcccctg cctttgccag tgaatcgcat gcaggtttct caagggatga attcatttaa
     3601 cccaatgtcc ctgggaaacg tccagttgcc acaggcaccc atgggacctc gtgcagcctc
     3661 ccctatgaac cactctgtgc agatgaacag catggcctca gttccgggta tggccatttc
     3721 tccttcacgg atgcctcagc ctccaaatat gatgggcact catgccaaca acattatggc
     3781 ccaggcacct actcagaacc agtttctgcc acagaaccag tttccatcat ccagtggggc
     3841 aatgagtgtg aacagtgtgg gcatggggca accagcagcc caggcaggtg tttcacaggg
     3901 tcaggtacct ggagctgctc tccctaaccc tctgaacatg ctggcacccc aggccagcca
     3961 gctgccttgc ccaccagtga cacagtcacc attgcacccg actccacctc ctgcttccac
     4021 agctgctggc atgccctctc tccaacatcc aacggcacca ggaatgaccc ctcctcagcc
     4081 agcagctccc actcagccat ctactcctgt gtcatctggg cagactccta ccccaactcc
     4141 tggctcagtg cccagcgctg cccaaacaca gagtacccct acagtccagg cagcagcaca
     4201 ggctcaggtg actccacagc ctcagacccc agtgcagcca ccatctgtgg ctactcctca
     4261 gtcatcacag cagcaaccaa cgcctgtgca tactcagcct cctggcacac cgctttctca
     4321 ggcagcagcc agcattgata atagagtccc tactccctcc tctgtgacca gtgctgaaac
     4381 cagttcccag cagccaggac ccgatgtgcc catgctggaa atgaagacag aggtgcagac
     4441 agatgatgct gagcctgaac ctactgaatc caagggggaa cctcggtctg agatgatgga
     4501 agaggattta caaggttctt cccaagtaaa agaagagaca gatacgacag agcagaagtc
     4561 agagccaatg gaagtagaag aaaagaaacc tgaagtaaaa gtggaagcta aagaggaaga
     4621 agagaacagt tcgaacgaca cagcctcaca atcaacatct ccttcccagc cacgcaaaaa
     4681 aatctttaaa cccgaggagc tacgccaggc acttatgcca actctagaag cactctatcg
     4741 acaggaccca gagtctttgc cttttcgtca gcctgtagat cctcagctcc taggaatccc
     4801 agattatttt gatatagtga agaatcctat ggacctttct accatcaaac gaaagctgga
     4861 cacagggcaa tatcaagaac cctggcagta tgtggatgat gtctggctta tgttcaacaa
     4921 tgcgtggcta tataatcgta aaacgtcccg tgtatataaa ttttgcagta aacttgcaga
     4981 ggtctttgaa caagaaattg accctgtcat gcagtctctt ggatattgct gtggacgaaa
     5041 gtatgagttc tccccacaga ctttgtgctg ttacggaaag cagctgtgta caattcctcg
     5101 tgatgcagcc tactacagct atcagaatag gtatcatttc tgtgagaagt gtttcacaga
     5161 gatccagggc gagaatgtga ccctgggtga cgacccttcc caacctcaga cgacaatttc
     5221 caaggatcaa tttgaaaaga agaaaaatga taccttagat cctgaacctt ttgttgactg
     5281 caaagagtgt ggccggaaga tgcatcagat ttgtgttcta cactatgaca tcatttggcc
     5341 ttcaggtttt gtgtgtgaca actgtttgaa gaaaactggc agacctcgga aagaaaacaa
     5401 attcagtgct aagaggctgc agaccacacg attgggaaac cacttagaag acagagtgaa
     5461 taagtttttg cggcgccaga atcaccctga agctggggag gtttttgtca gagtggtggc
     5521 cagctcagac aagactgtgg aggtcaagcc gggaatgaag tcaaggtttg tggattctgg
     5581 agagatgtcg gaatctttcc catatcgtac caaagcactc tttgcttttg aggagatcga
     5641 tggagtcgat gtgtgctttt ttgggatgca tgtgcaagaa tacggctctg attgcccccc
     5701 accaaataca aggcgtgtat acatatctta tgcggctagc attcatttct tccggccccg
     5761 ctgcctccgg acagctgttt accatgagat cctcatcgga tatctcgagt atgtgaagaa
     5821 attggggtat gtgacaggac atatttgggc ctgtccccca agtgaaggag atgactatat
     5881 ctttcattgc cacccccctg accagaaaat ccccaaacca aaacgactac aggagtggta
     5941 caagaagatg ctggacaagg cgtttgcaga gaggatcatt aacgactata aggacatctt
     6001 caaacaagcg aacgaagaca ggctcacgag tgccaaggag ttgccctatt ttgaaggaga
     6061 tttctggcct aatgtgttgg aagaaagcat taaggaacta gaacaagaag aagaagaaag
     6121 gaaaaaagaa gagagtactg cagcgagtga gactcctgag ggcagtcagg gtgacagcaa
     6181 aaatgcgaag aaaaagaaca acaagaagac caacaaaaac aaaagcagca ttagccgcgc
     6241 caacaagaag aagcccagca tgcccaatgt ttccaacgac ctgtcgcaga agctgtatgc
     6301 caccatggag aagcacaagg aggtattctt tgtgattcat ctgcatgctg ggcctgttat
     6361 cagcactcag ccccccatcg tggaccctga tcctctgctt agctgtgacc tcatggatgg
     6421 gcgagatgcc ttcctcaccc tggccagaga caagcactgg gaattctctt ccttacgccg
     6481 ctccaaatgg tccactctgt gcatgctggt ggagctgcac acacagggcc aggaccgctt
     6541 tgtttatacc tgcaatgagt gcaaacacca tgtggaaaca cgctggcact gcactgtgtg
     6601 tgaggactat gacctttgta tcaattgcta caacacaaag agccacaccc ataagatggt
     6661 gaagtggggg ctaggcctag atgatgaggg cagcagtcag ggtgagccac agtccaagag
     6721 cccccaggaa tcccggcgtc tcagcatcca gcgctgcatc cagtccctgg tgcatgcctg
     6781 ccagtgtcgc aatgccaact gctcactgcc gtcttgccag aagatgaagc gagtcgtgca
     6841 gcacaccaag ggctgcaagc gcaagactaa tggaggatgc ccagtgtgca agcagctcat
     6901 tgctctttgc tgctaccacg ccaaacactg ccaagaaaat aaatgccctg tgcccttctg
     6961 cctcaacatc aaacataagc tccgccagca gcagatccag catcgcctgc agcaggctca
     7021 gctcatgcgc cggcgaatgg caaccatgaa cacccgcaat gtgcctcagc agagtttgcc
     7081 ttctcctacc tcagcaccac ccgggactcc tacacagcag cccagcacac cccaaacacc
     7141 acagccccca gcccagcctc agccttcacc tgttaacatg tcaccagctg gcttccctaa
     7201 tgtagcccgg actcagcccc caacaatagt gtctgctggg aagcctacca accaggtgcc
     7261 agctccccca ccccctgccc agcccccacc tgcagcagta gaagcagccc ggcaaattga
     7321 acgtgaggcc cagcagcagc agcacctata ccgagcaaac atcaacaatg gcatgccccc
     7381 aggacgtgca ggtatgggga ccccaggaag ccaaatgact cctgtgggcc tgaatgtgcc
     7441 ccgtcccaac caagtcagtg ggcctgtcat gtctagtatg ccacctgggc agtggcagca
     7501 ggcacccatc cctcagcagc agccgatgcc aggcatgccc aggcctgtaa tgtccatgca
     7561 ggcccaggca gcagtggctg ggccacggat gcccaatgtg cagccaccaa ggagcatctc
     7621 gccaagtgcc ctgcaagacc tgctacggac cctaaagtca cccagctctc ctcagcagca
     7681 gcagcaggtg ctgaacatcc ttaaatcaaa cccacagcta atggcagctt tcatcaaaca
     7741 gcgcacagcc aagtatgtgg ccaatcagcc tggcatgcag ccccagcccg gacttcaatc
     7801 ccagcctggt atgcagcccc agcctggcat gcaccagcag cctagtttgc aaaacctgaa
     7861 cgcaatgcaa gctggtgtgc cacggcctgg tgtgcctcca ccacaaccag caatgggagg
     7921 cctgaatccc cagggacaag ctctgaacat catgaaccca ggacacaacc ccaacatgac
     7981 aaacatgaat ccacagtacc gagaaatggt gaggagacag ctgctacagc accagcagca
     8041 gcagcagcaa cagcagcagc agcagcagca acaacaaaat agtgccagct tggccggggg
     8101 catggcggga cacagccagt tccagcagcc acaaggacct ggaggttatg ccccagccat
     8161 gcagcagcaa cgcatgcaac agcacctccc catccagggc agctccatgg gccagatggc
     8221 tgctccaatg ggacaacttg gccagatggg gcagcctggg ctaggggcag acagcacccc
     8281 taatatccag caggccctgc agcaacggat tctgcagcag cagcagatga agcaacaaat
     8341 tgggtcacca ggccagccga accccatgag cccccagcag cacatgctct caggacagcc
     8401 acaggcctca catctccctg gccagcagat cgccacatcc cttagtaacc aggtgcgatc
     8461 tccagcccct gtgcagtctc cacggcccca atcccaacct ccacattcca gcccgtcacc
     8521 acggatacaa ccccagcctt caccacacca tgtttcaccc cagactggtt cccctcaccc
     8581 tggactcgca gtcaccatgg ccagctccat ggatcaggga cacctgggga accctgaaca
     8641 gagtgcaatg ctcccccagc tgaatacccc caacaggagc gcactgtcca gtgaactgtc
     8701 cctggttggt gataccacgg gagacacact agaaaagttt gtggagggtt tgaagctgga
     8761 tccggggatg gcctacccat acgacgtgcc tgactacgcc tcctagcggc cgctcgagca
     8821 tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct
     8881 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     8941 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     9001 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     9061 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     9121 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     9181 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     9241 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     9301 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     9361 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     9421 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     9481 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     9541 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     9601 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9661 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9721 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     9781 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9841 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     9901 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     9961 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
    10021 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
    10081 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
    10141 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
    10201 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
    10261 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
    10321 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
    10381 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
    10441 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
    10501 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
    10561 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
    10621 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
    10681 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
    10741 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
    10801 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
    10861 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
    10921 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
    10981 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    11041 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
    11101 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    11161 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    11221 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    11281 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    11341 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    11401 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    11461 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    11521 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    11581 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    11641 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    11701 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    11761 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    11821 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    11881 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    11941 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    12001 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    12061 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    12121 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    12181 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    12241 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    12301 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    12361 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    12421 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    12481 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    12541 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    12601 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    12661 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    12721 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    12781 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    12841 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    12901 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    12961 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    13021 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    13081 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    13141 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    13201 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    13261 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
    13321 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
    13381 gtgtgttgga ggtcgctgag tagtgcgcga gcaa
//
