LOCUS       sequence-243417-        3897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part P- Promoter (non-constitutive); T7 promoter; recognized 
            by T7 polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-T7pro_P
FEATURES             Location/Qualifiers
     source          1..3897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     86..105
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        86..104
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    130..150
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             178..198
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             218..925
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             218..925
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(242..270)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     865..885
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      965..1059
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1132..1223
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1224..1292,1293..2084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2088..2127
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2274..2382
                     /note="incP origin of transfer"
     rep_origin      2418..3006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2907..2926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3160..3177
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3192..3332
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3258..3277)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3418..3440
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3434..3625)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3805..3891)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tggagtaata cgactcacta tagggtactt gagacctttc
      121 atcgcgacct actagtagcg gccgctgcag ggagtaactt ctgactgagt tgcatacttg
      181 tttaacttta agaaggagat atacaatggt agcccgtaaa ggagaagaac ttttcactgg
      241 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      301 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      361 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
      421 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
      481 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
      541 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
      601 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
      661 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
      721 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
      781 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
      841 tcccaacgaa aagagagatc acatggtcct tcttgagttt gtaacagctg ctgggattac
      901 acatggcatg gatgaactat acaaaggttc gtaacttctg actgagttgc acgctctagt
      961 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     1021 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac
     1081 gatttaaatt agtagcccgc ctaatgagcg ggcttttttt taattcccct atttgtttat
     1141 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1201 aataatattg aaaaaggaag agtatgagca ttcagcattt tcgtgtggcg ctgattccgt
     1261 tttttgcggc gttttgcctg ccggtgtttg cgcatccgga aaccctggtg aaagtgaaag
     1321 atgcggaaga tcaactgggt gcgcgcgtgg gctatattga actggatctg aacagcggca
     1381 aaattctgga atcttttcgt ccggaagaac gttttccgat gatgagcacc tttaaagtgc
     1441 tgctgtgcgg tgcggttctg agccgtgtgg atgcgggcca ggaacaactg ggccgtcgta
     1501 ttcattatag ccagaacgat ctggtggaat atagcccggt gaccgaaaaa catctgaccg
     1561 atggcatgac cgtgcgtgaa ctgtgcagcg cggcgattac catgagcgat aacaccgcgg
     1621 cgaacctgct gctgacgacc attggcggtc cgaaagaact gaccgcgttt ctgcataaca
     1681 tgggcgatca tgtgacccgt ctggatcgtt gggaaccgga actgaacgaa gcgattccga
     1741 acgatgaacg tgataccacc atgccggcag caatggcgac caccctgcgt aaactgctga
     1801 cgggtgagct gctgaccctg gcaagccgcc agcaactgat tgattggatg gaagcggata
     1861 aagtggcggg tccgctgctg cgtagcgcgc tgccggctgg ctggtttatt gcggataaaa
     1921 gcggtgcggg cgaacgtggc agccgtggca ttattgcggc gctgggcccg gatggtaaac
     1981 cgagccgtat tgtggtgatt tataccaccg gcagccaggc gacgatggat gaacgtaacc
     2041 gtcagattgc ggaaattggc gcgagcctga ttaaacattg gtaaaccgat acaattaaag
     2101 gctccttttg gagccttttt ttttggacga cccttgtcct tttccgctgc ataaccctgc
     2161 ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga
     2221 ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg
     2281 ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac
     2341 ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg ccccgtagaa
     2401 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2461 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2521 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     2581 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2641 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2701 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2761 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2821 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2881 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2941 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3001 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3061 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3121 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3181 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     3241 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     3301 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     3361 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg acagtctccg
     3421 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     3481 aaagctcatc agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca tccgcgtcca
     3541 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     3601 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     3661 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
     3721 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggggc
     3781 gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     3841 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcct
//
