LOCUS       sequence-243471-        2582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Part. CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie 
            MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick
            AJ
  TITLE     CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria 
            Based on the Plant MoClo Syntax.
  JOURNAL   Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub
            2019 Feb 28.
  PUBMED    30819783
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.18.01401"; journalName: "Plant Physiol"; date: 
            "2019-05"; volume: "180"; issue: "1"; pages: "39-55"
COMMENT     Sequence Label: pC0.026
FEATURES             Location/Qualifiers
     source          1..2582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..446
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(447..533,534..1868)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 cacatatacc tgccgttcac tattatttag tgaaatgaga tattatgata ttttctgaat
       61 tgtgattaaa aaggcaactt tatgcccatg caacagaaac tataaaaaat acagagaatg
      121 aaaagaaaca gatagatttt ttagttcttt aggcccgtag tctgcaaatc cttttatgat
      181 tttctatcaa acaaaagagg aaaatagacc agttgcaatc caaacgagag tctaatagaa
      241 tgaggtcgaa aagtaaatcg cgcgggtttg ttactgataa agcaggcaag acctaaaatg
      301 tgtaaagggc aaagtgtata ctttggcgtc accccttaca tattttaggt ctttttttat
      361 tgtgcgtaac taacttgcca tcttcaaaca ggagggctgg aagaagcaga ccgctaacac
      421 agtacataaa aaaggagaca tgaacgatga acatcaaaaa gtttgcaaaa caagcaacag
      481 tattaacctt tactaccgca ctgctggcag gaggcgcaac tcaagcgttt gcgaaagaaa
      541 cgaaccaaaa gccatataag gaaacatacg gcatttccca tattacacgc catgatatgc
      601 tgcaaatccc tgaacagcaa aaaaatgaaa aatatcaagt tcctgaattc gattcgtcca
      661 caattaaaaa tatctcttct gcaaaaggcc tggacgtttg ggacagctgg ccattacaaa
      721 acgctgacgg cactgtcgca aactatcacg gctaccacat cgtctttgca ttagccggag
      781 atcctaaaaa tgcggatgac acatcgattt acatgttcta tcaaaaagtc ggcgaaactt
      841 ctattgacag ctggaaaaac gctggccgcg tctttaaaga cagcgacaaa ttcgatgcaa
      901 atgattctat cctaaaagac caaacacaag aatggtcagg ttcagccaca tttacatctg
      961 acggaaaaat ccgtttattc tacactgatt tctccggtaa acattacggc aaacaaacac
     1021 tgacaactgc acaagttaac gtatcagcat cagacagctc tttgaacatc aacggtgtag
     1081 aggattataa atcaatcttt gacggtgacg gaaaaacgta tcaaaatgta cagcagttca
     1141 tcgatgaagg caactacagc tcaggcgaca accatacgct gagagatcct cactacgtag
     1201 aagataaagg ccacaaatac ttagtatttg aagcaaacac tggaactgaa gatggctacc
     1261 aaggcgaaga atctttattt aacaaagcat actatggcaa aagcacatca ttcttccgtc
     1321 aagaaagtca aaaacttctg caaagcgata aaaaacgcac ggctgagtta gcaaacggcg
     1381 ctctcggtat gattgagcta aacgatgatt acacactgaa aaaagtgatg aaaccgctga
     1441 ttgcatctaa cacagtaaca gatgaaattg aacgcgcgaa cgtctttaaa atgaacggca
     1501 aatggtacct gttcactgac tcccgcggat caaaaatgac gattgacggc attacgtcta
     1561 acgatattta catgcttggt tatgtttcta attctttaac tggcccatac aagccgctga
     1621 acaaaactgg ccttgtgtta aaaatggatc ttgatcctaa cgatgtaacc tttacttact
     1681 cacacttcgc tgtacctcaa gcgaaaggaa acaatgtcgt gattacaagc tatatgacaa
     1741 acagaggatt ctacgcagac aaacaatcaa cgtttgcgcc aagcttcctg ctgaacatca
     1801 aaggcaagaa aacatctgtt gtcaaagaca gcatccttga acaaggacaa ttaacagtta
     1861 acaaataaaa acgcaaaaga aaatgccgat atcctattgg cattttcttt tatttcttat
     1921 caacataaag gtgaatccca tatgaactat ataaaagcag gcaaatggct aaccgtattc
     1981 ctaacctttt ggtaatgact ccaacttatt gatagtgttt tatgttcaga taatgcccga
     2041 tgactttgtc atgcagctcc accgattttg agaacgacag cgacttccgt cccagccgtg
     2101 ccaggtgctg cctcagattc aggttatgcc gctcaattcg ctgcgtatat cgcttgctga
     2161 ttacgtgcag ctttcccttc aggcgggatt catacagcgg ccagccatcc gtcatccata
     2221 tcaccacgtc aaagggtgac agcaggctca taagacgccc cagcgtcgcc atagtgcgtt
     2281 caccgaatac gtgcgcaaca accgtctacc ggagactgtc atacgcgtaa aacagccagc
     2341 gctggcgcga tttagccccg acatagcccc actgttcgtc catttccgcg cagacgatga
     2401 cgtcactgcc cggctgtatg cgcgaggtta ccgactgcgg cctgagtttt ttaagtgacg
     2461 taaaatcgtg ttgaggccaa cgcccataat gcgggctgtt gcccggcatc caacgccatt
     2521 catggccata tcaatgattt tctggtgcgt accgggttga gaagcggtgt aagtgaactg
     2581 ca
//
