LOCUS       sequence-243558-        4538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for making zebrafish apoBa in situ probe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Otis JP, Zeituni EM, Thierer JH, Anderson JL, Brown AC, Boehm ED, 
            Cerchione DM, Ceasrine AM, Avraham-Davidi I, Tempelhof H, Yaniv K, 
            Farber SA
  TITLE     Zebrafish as a model for apolipoprotein biology: comprehensive 
            expression analysis and a role for ApoA-IV in regulating food 
            intake.
  JOURNAL   Dis Model Mech. 2015 Mar;8(3):295-309. doi: 10.1242/dmm.018754. Epub
            2015 Jan 29.
  PUBMED    25633982
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dmm.018754"; journalName: "Dis Model Mech"; date: 
            "2015-03"; volume: "8"; issue: "3"; pages: "295-309"
COMMENT     Sequence Label: PCRII apoBa
FEATURES             Location/Qualifiers
     source          1..4538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(767..786)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(768..786)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(793..810)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(793..809)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(802..824)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      950..1378
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1037..1056)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1247..1268
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             1722..2516
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1776..1795)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2386..2405
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(2534..2602,2603..3394)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2752..2771)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3565..4153
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4054..4073
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4307..4324
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4441..4462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4477..4482,4483..4500,4501..4507)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4515..4531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(4520..4538,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatttag gtgacactat agaatactca
       61 agctatgcat caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg
      121 gaattcggct ttcagtttcc cgtccctcac gacatacgct gggcattgac attgcaagcc
      181 agacattttc tgatgtgagc tttcgctatg catcacacaa caatgggatc acgtcttcag
      241 tatcctcacc agcagctgga ttcattgggt tccagttcac taggaggtca ccatctcagt
      301 tttatgcaaa actgttcagc agatatttgt caacatctga caaggacaca gacctcttga
      361 gtttaaagat gactttgaag aactcggaaa agctgagcgt tcaggttgga tatcatttca
      421 gtggactctc agacatgata aatggcctga aagacagact tccttccatc ttagcagcac
      481 ttcaaaagtt cataaaccag taccacattg atcaccttgg catggacata aatcgtgctg
      541 ccttaaagct gaagaacgca ctctccaatg gcattgacaa agcttacttg gagatccctc
      601 gcatgttcga tgccacccat acctcattcg agcaagtcaa acaacacggt aataagatgt
      661 ggaggaagat gttggagagc tttccccagg atgatcttaa gccgaattct gcagatatcc
      721 atcacactgg cggccgctcg agcatgcatc tagagggccc aattcgccct atagtgagtc
      781 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      841 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
      901 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt
      961 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     1021 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     1081 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     1141 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     1201 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     1261 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     1321 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     1381 aacaaaattc agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc
     1441 agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg
     1501 caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg
     1561 cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg
     1621 ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg
     1681 gatcaagatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     1741 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     1801 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     1861 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     1921 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     1981 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatcccacc
     2041 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     2101 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     2161 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     2221 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     2281 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     2341 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     2401 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     2461 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattg
     2521 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     2581 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     2641 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     2701 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     2761 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     2821 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     2881 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     2941 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3001 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3061 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3121 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3181 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3241 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3301 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3361 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     3421 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     3481 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     3541 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3601 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3661 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3721 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3781 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3841 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3901 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3961 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4021 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4081 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4141 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4201 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     4261 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     4321 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     4381 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     4441 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     4501 tatgttgtgt ggaattgtga gcggataaca atttcaca
//
