LOCUS       sequence-243564-        4715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for making zebrafish apoA-Ib in situ probe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Otis JP, Zeituni EM, Thierer JH, Anderson JL, Brown AC, Boehm ED, 
            Cerchione DM, Ceasrine AM, Avraham-Davidi I, Tempelhof H, Yaniv K, 
            Farber SA
  TITLE     Zebrafish as a model for apolipoprotein biology: comprehensive 
            expression analysis and a role for ApoA-IV in regulating food 
            intake.
  JOURNAL   Dis Model Mech. 2015 Mar;8(3):295-309. doi: 10.1242/dmm.018754. Epub
            2015 Jan 29.
  PUBMED    25633982
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dmm.018754"; journalName: "Dis Model Mech"; date: 
            "2015-03"; volume: "8"; issue: "3"; pages: "295-309"
COMMENT     Sequence Label: PCRII apoA-Ib
FEATURES             Location/Qualifiers
     source          1..4715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(944..963)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(945..963)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(970..987)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(970..986)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(979..1001)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1127..1555
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1214..1233)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1424..1445
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             1899..2693
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1953..1972)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2563..2582
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(2711..2779,2780..3571)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2929..2948)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3742..4330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4231..4250
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4484..4501
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4618..4639
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4654..4659,4660..4677,4678..4684)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4692..4708
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(4697..4715,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatttag gtgacactat agaatactca
       61 agctatgcat caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg
      121 gaattcgccc ttcgaacttc tggagggcct tgaagatgcc ctcgaagtgt ggcttcacct
      181 gctctcccag ctcggtcagc tctttcctca gttctccaga cttcaccttc tctctgaact
      241 ccaaggctcc cttctccatc tggtccttgt actcctggac gtagggctcc agcatggtcc
      301 tcagctcctg cacgttctca gccaccttgc ctcggatggc ctccaggatg ggcatcagct
      361 tggacttggt ctcctcccag ttgggtccga tcttctcctt caggctgagg accacaggct
      421 ccagcttggc cctgacctcc tccatgtgct gacgctgctt gaccaggtag tcgtccatga
      481 agggcttcag ctcgtccctg tactcattga agtgtttctc cagcacggct ctgagctcct
      541 cgcgcttggg ctcgatgtcc ttgcgcaggt cctccatgtc cttgttcagc ttctcgcgga
      601 tggggttggt ggcgtccatc acctgagagc ccagaggcgt gatgacctgg aagaagttct
      661 ggaagtggga atggaggttg tcgatggact ggcccaggaa gactttgtag tctttgaact
      721 cggtgtcgtc gaggtgagtg agggacttgt gtgcggcctg cttcagatga tctgcataaa
      781 cctgcagtgc agacttcaca tgctccagct gcgatggggg ctcgtcctgc aggaaccggg
      841 cctggcaacc tgccaggaat acggtgaggg cgagaagggc gaattctgca gatatccatc
      901 acactggcgg ccgctcgagc atgcatctag agggcccaat tcgccctata gtgagtcgta
      961 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     1021 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     1081 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc
     1141 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     1201 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     1261 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     1321 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     1381 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     1441 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     1501 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     1561 aaaattcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga
     1621 aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa
     1681 gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg
     1741 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga
     1801 agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat
     1861 caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     1921 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     1981 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     2041 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     2101 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     2161 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tcccaccttg
     2221 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     2281 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     2341 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     2401 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     2461 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     2521 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     2581 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     2641 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaattgaaa
     2701 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2761 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2821 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2881 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2941 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3001 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3061 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3121 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3181 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3241 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3301 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3361 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3421 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3481 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3541 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3601 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3661 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3721 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3781 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3841 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3901 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3961 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4021 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4081 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4141 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4201 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4261 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4321 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4381 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4441 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4501 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     4561 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     4621 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     4681 gttgtgtgga attgtgagcg gataacaatt tcaca
//
