LOCUS       sequence-243721-        8593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the fluorescent protein mNeonGreen2 in a lentiviral 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Wieland FC, Sthijns MMJPE, Geuens T, van Blitterswijk CA, LaPointe 
            VLS
  TITLE     The Role of Pancreatic Alpha Cells and Endothelial Cells in the 
            Reduction of Oxidative Stress in Pseudoislets.
  JOURNAL   Front Bioeng Biotechnol. 2021 Sep 9;9:729057. doi: 
            10.3389/fbioe.2021.729057. eCollection 2021.
  PUBMED    34568302
REFERENCE   2  (bases 1 to 8593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2021-09-9"; volume: "9:729057. doi"; 
            pages: " 10.3389/fbioe.2021.729057. eCollection 2021"
COMMENT     Sequence Label: pLenti6.2_mNeonGreen2
FEATURES             Location/Qualifiers
     source          1..8593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    210..326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        349..652
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        653..856
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     807..827
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     853..877
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      950..959
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1211..1220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1660..2248
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1713..1733)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2131..2142)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2262..2762
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2297..2320)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2695..2714
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2783..3382
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2783..2802)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3279..3299
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3520..3753
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3825..3946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3862..3881)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3916..3935
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3986..4121
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4141..4160)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4142..4160)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4170..4187)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4170..4186)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4179..4201)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4328..4783
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4415..4434)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4625..4646
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4809..4913
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4914..4982,4983..5774)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5132..5151)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5945..6533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6434..6453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6687..6704
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6821..6842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6857..6862,6863..6880,6881..6887)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6895..6911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6900..6922
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6919..6935
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6919..6935
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6954..6974
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6956..6974
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7002..7228
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7229..7409
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7456..7581
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8074..8307
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8492..8536
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
       61 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
      121 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
      181 gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac
      241 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
      301 aaacaaatta caaaattcaa aattttatcg ataagcttgg gagttccgcg ttacataact
      361 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      421 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      481 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      541 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      601 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      661 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      721 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      781 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      841 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
      901 ttttgacctc catagaagac accgactcta gagatatctt cgaaggcgcg ccaccatggt
      961 gagcaagggt gaggaggata acatggcctc tctcccagcg actcatgagt tacacatctt
     1021 tggctccatc aacggtgtgg actttgacat ggtgggtcag ggtaccggca atccaaatga
     1081 tggttatgag gagttaaacc tgaagtccac caagggtgac ctccagttct ccccctggat
     1141 tctggtccct catatcgggt atggcttcca tcagtacctg ccctaccctg acgggatgtc
     1201 gcctttccag gccgccatgg tagatggctc cggataccaa gtccatcgca caatgcagtt
     1261 tgaagatggt gcctccctta ctgttaacta ccgctacacc tacgagggaa gccacatcaa
     1321 aggagaggcc caggtgatgg ggactggttt ccctgctgac ggtcctgtga tgaccaacac
     1381 gctgaccgct gcggactggt gcatgtcgaa gaagacttac cccaacgaca aaaccatcat
     1441 cagtaccttt aagtggagtt acaccactgt aaatggcaaa cgctaccgga gcactgcgcg
     1501 gaccacctac acctttgcca agccaatggc ggctaactat ctgaagaacc agccgatgta
     1561 cgtgttccgt aagacggagc tcaagcactc catgaccgag ctcaacttca aggagtggca
     1621 aaaggccttt accgatatga tgtaagcggc cgcgtcgaca atcaacctct ggattacaaa
     1681 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     1741 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     1801 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     1861 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     1921 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     1981 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     2041 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     2101 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     2161 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     2221 cggatctccc tttgggccgc ctccccgcct ggaattctac cgggtagggg aggcgctttt
     2281 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     2341 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     2401 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     2461 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     2521 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     2581 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggncgggc
     2641 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     2701 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     2761 cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     2821 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     2881 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     2941 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     3001 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     3061 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     3121 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     3181 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     3241 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     3301 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     3361 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
     3421 cgcacgaccc catgcatctc gagggcccgg tacctttaag accaatgact tacaaggcag
     3481 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta gctcactccc
     3541 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     3601 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3661 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3721 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     3781 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     3841 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3901 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     3961 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     4141 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     4201 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4261 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4321 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4381 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4441 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4501 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4561 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4621 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4681 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4741 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     4801 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4861 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4921 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4981 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5041 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5101 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5161 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5221 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5281 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5341 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5401 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5461 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5521 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5581 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5641 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5701 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5761 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5821 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5881 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5941 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6001 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6061 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6121 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6181 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6241 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6301 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6361 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6421 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6481 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6541 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6601 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6661 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6721 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6781 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6841 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     6901 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     6961 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     7021 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     7081 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     7141 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     7201 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     7261 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7321 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7381 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     7441 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     7501 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     7561 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     7621 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     7681 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     7741 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     7801 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     7861 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     7921 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     7981 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     8041 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     8101 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     8161 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     8221 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     8281 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     8341 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     8401 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     8461 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     8521 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     8581 attcataatg ata
//
