LOCUS       sequence-243746-        9274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral plasmid to co-express a guide RNA and 
            mOrange.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Giuliano CJ, Lin A, Girish V, Sheltzer JM
  TITLE     Generating Single Cell-Derived Knockout Clones in Mammalian Cells 
            with CRISPR/Cas9.
  JOURNAL   Curr Protoc Mol Biol. 2019 Sep;128(1):e100. doi: 10.1002/cpmb.100.
  PUBMED    31503414
REFERENCE   2  (bases 1 to 9274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol."; date: "2019-09"; volume: "128(1):e100. doi"; pages: " 
            10.1002/cpmb.100"
COMMENT     Sequence Label: LRG2.1-mOrange
FEATURES             Location/Qualifiers
     source          1..9274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    89..110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(125..130,131..148,149..155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     187..203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     222..242
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        224..242
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        270..496
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             497..677
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    724..849
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1342..1575
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1760..1804
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1982..2230
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1982..2002
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2153..2172
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    4256..4373
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4442..4653
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4673..5380
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGF
                     QTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     5083..5102
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5396..5984
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5449..5469)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5867..5878)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6056..6289
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6367..6488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6404..6423)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6458..6477
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6528..6663
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6683..6702)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6684..6702)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6712..6729)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6712..6728)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6721..6743)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6870..7325
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6957..6976)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7167..7188
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7351..7455
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7456..7524,7525..8316)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7674..7693)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8487..9075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8976..8995
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9229..9246
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      181 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
      241 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
      301 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
      361 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
      421 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
      481 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
      541 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
      601 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
      661 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
      721 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
      781 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
      841 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
      901 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
      961 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     1021 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1081 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1141 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     1201 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     1261 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     1321 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     1381 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     1441 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     1501 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     1561 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     1621 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     1681 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     1741 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     1801 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     1861 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     1921 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccag
     1981 agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     2041 gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     2101 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     2161 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     2221 acgaaacacc ggagacggtt gtaaatgagc acacaaaata cacatgctaa aatattatat
     2281 tctatgacct ttataaaatc aaccaaaatc ttctttttaa taactttagt atcaataatt
     2341 agaattttta tgttcctttt tgcaaacttt taataaaaat gagcaaaata aaaaaacgct
     2401 agttttagta actcgcgttg ttttcttcac ctttaataat agctactcca ccacttgttc
     2461 ctaagcggtc agctcctgct tcaatcattt tttgagcatc ttcaaatgtt ctaactccac
     2521 cagctgcttt aactaaagca ttgtctttaa caactgactt cattagttta acatcttcaa
     2581 atgttgcacc tgattttgaa aatcctgttg atgttttaac aaattctaat ccagcttcaa
     2641 cagctatttc acaagctttc atgatttctt cttttgttaa taaacaattt tccataatac
     2701 atttaacaac atgtgatcca gctgcttttt ttacagcttt catgtcttct aaaactaatt
     2761 cataattttt gtcttttaat gcaccaatat ttaataccat atcaatttct gttgcaccat
     2821 ctttaattgc ttcagaaact tcgaatgctt ttgtagctgt tgtgcatgca cctagaggaa
     2881 aacctacaac atttgttatt cctacatttg tgccttttaa taattcttta caatagcttg
     2941 ttcaatatga attaacacaa actgttgcaa aatcaaattc aattgcttca tcacataatt
     3001 gtttaatttc agctttcgta gcatcttgtt ttaataatgt gtgatctata tatttgttta
     3061 gtttcatttt ttctcctata tattcatttt taattttaat tctttaataa tttcgtctac
     3121 tttaacttta gcgttttgaa cagattcacc aacacctata aaataaattt ttagtttagg
     3181 ttcagttcca cttgggcgaa cagcaaatca tgacttatct tctaaataaa attttagtaa
     3241 gtcttgtcct ggcatattat acattccatc gatgtagtct tcaacattaa caactttaag
     3301 tccagcaatt tgagttaagg gtgttgctct caatgatttc attaatggtt caatttttaa
     3361 tttcttttct tctggtttaa aattcaagtt taaagtgaaa gtgtaatatg cacccatttc
     3421 tttaaataaa tcttctaaat agtctactaa tgttttattt tgttttttat aaaatcaagc
     3481 agcctctgct attaatatag aagcttgtat tccatcttta tctctagctg agtcatcaat
     3541 tacatatcca taactttctt cataagcaaa aacaaaattt aatccgttat cttcttcttt
     3601 agcaatttct ctacccattc atttaaatcc agttaaagtt tttacaatat taactccata
     3661 tttttcatga gcgattctat cacccaaatc acttgttaca aaacttgaat atagagccgg
     3721 attttttgga atgctattta agcgttttag atttgataat tttcaatcaa ttaaaattgg
     3781 tcctgtttga tttccatcta atcttacaaa atgaccatca tgttttattg ccattccaaa
     3841 tctgtcagca tctgggtcat tcataataat aatatctgca tcatgtttaa taccatattc
     3901 aagcggtatt tttcatgcag gatcaaattc tggatttgga tttacaacat ttttaaatgt
     3961 ttcatcttca aatgcatgct cttcaacctc aataacgtta tatcctgatt cacgtaatat
     4021 ttttggggta aatttagttc ctgttccatt aactgcgcta aaaataattt ttaaatcttt
     4081 tttagcttct tgctcttttt tgtacgtctc tgtttaagag ctatgctgga aacagcatag
     4141 caagtttaaa taaggctagt ccgttatcaa ctttgctgga aacagcaaag tggcaccgag
     4201 tcggtgcttt ttttgaattc tagatcttga gacaaatggc agtattcatc cacaatttta
     4261 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     4321 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     4381 attacaggga cagcagagat ccactttggc gccggctcga gtggctccgg tgcccgtcag
     4441 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     4501 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     4561 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     4621 ctttttcgca acgggtttgc cgccagaaca caggtgtcgt gacgcgggat ccgtgagcaa
     4681 gggcgaggag aataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcgcatgga
     4741 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     4801 gggctttcag accgctaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     4861 catcctgtcc cctcagttca cctacggctc caaggcctac gtgaagcacc ccgccgacat
     4921 ccccgactac ttcaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     4981 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctccaggacg gcgagttcat
     5041 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     5101 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     5161 cgagatcaag atgaggctga agctgaagga cggcggccac tacacctccg aggtcaagac
     5221 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacatcgtcg gcatcaagtt
     5281 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     5341 ccgccactcc accggcggca tggacgagct gtacaagtag acgcgttaag tcgacaatca
     5401 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5461 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5521 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5581 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5641 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5701 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5761 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     5821 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5881 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     5941 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     6001 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6061 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt
     6301 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     6361 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6421 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6481 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     6541 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6601 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6661 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     6721 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6781 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6841 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     6901 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6961 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7021 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7081 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7141 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7201 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7261 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     7321 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7381 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7441 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7501 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7561 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7621 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7681 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7741 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7801 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7861 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7921 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7981 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8041 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8101 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8161 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8221 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8281 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8341 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8401 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8461 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8521 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8581 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8641 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8701 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8761 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8821 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8881 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8941 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9001 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9061 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9121 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9181 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9241 agcgaggaag cggaagagcg cccaatacgc aaac
//
