LOCUS       sequence-243921-       13452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses multimeric human VWF protein without A1 domain. This 
            construct has no tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13452)
  AUTHORS   Zhang C, Kelkar A, Neelamegham S
  TITLE     von Willebrand factor self-association is regulated by the 
            shear-dependent unfolding of the A2 domain.
  JOURNAL   Blood Adv. 2019 Apr 9;3(7):957-968. doi: 
            10.1182/bloodadvances.2018030122.
  PUBMED    30936056
REFERENCE   2  (bases 1 to 13452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/bloodadvances"; journalName: "Blood Adv"; date: "2019-04-9-
            9"; volume: "3"; issue: "7"; pages: "957-968"
COMMENT     Sequence Label: pcDNA3.1-deltaA1-VWF
FEATURES             Location/Qualifiers
     source          1..13452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        74..453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        454..657
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     608..628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     702..721
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        702..720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2379..2390
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(8713..8730)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8719..8943
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8989..9417
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9076..9095)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9286..9307
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9426..9446)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9431..9760
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9611..9746
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9673..9692
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9809..10834
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    10964..11085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11001..11020)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11055..11074
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11134..11150)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11134..11150)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11147..11169)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11158..11174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11182..11188,11189..11206,11207..11212))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11227..11248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11365..11382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11536..12124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11616..11635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12295..13086,13087..13155))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12918..12937
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13156..13260)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13335..13354)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
       61 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
      661 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
      721 gagacccaag ctggctagcg tttaaactta agctgatcca ctagtccagt gtggtggaat
      781 tcccgcagcc ctcatttgca ggggaagatg attcctgcca gatttgccgg ggtgctgctt
      841 gctctggccc tcattttgcc agggaccctt tgtgcagaag gaactcgcgg caggtcatcc
      901 acggcccgat gcagcctttt cggaagtgac ttcgtcaaca cctttgatgg gagcatgtac
      961 agctttgcgg gatactgcag ttacctcctg gcagggggct gccagaaacg ctccttctcg
     1021 attattgggg acttccagaa tggcaagaga gtgagcctct ccgtgtatct tggggaattt
     1081 tttgacatcc atttgtttgt caatggtacc gtgacacagg gggaccaaag agtctccatg
     1141 ccctatgcct ccaaagggct gtatctagaa actgaggctg ggtactacaa gctgtccggt
     1201 gaggcctatg gctttgtggc caggatcgat ggcagcggca actttcaagt cctgctgtca
     1261 gacagatact tcaacaagac ctgcgggctg tgtggcaact ttaacatctt tgctgaagat
     1321 gactttatga cccaagaagg gaccttgacc tcggaccctt atgactttgc caactcatgg
     1381 gctctgagca gtggagaaca gtggtgtgaa cgggcatctc ctcccagcag ctcatgcaac
     1441 atctcctctg gggaaatgca gaagggcctg tgggagcagt gccagcttct gaagagcacc
     1501 tcggtgtttg cccgctgcca ccctctggtg gaccccgagc cttttgtggc cctgtgtgag
     1561 aagactttgt gtgagtgtgc tggggggctg gagtgcgcct gccctgccct cctggagtac
     1621 gcccggacct gtgcccagga gggaatggtg ctgtacggct ggaccgacca cagcgcgtgc
     1681 agcccagtgt gccctgctgg tatggagtat aggcagtgtg tgtccccttg cgccaggacc
     1741 tgccagagcc tgcacatcaa tgaaatgtgt caggagcgat gcgtggatgg ctgcagctgc
     1801 cctgagggac agctcctgga tgaaggcctc tgcgtggaga gcaccgagtg tccctgcgtg
     1861 cattccggaa agcgctaccc tcccggcacc tccctctctc gagactgcaa cacctgcatt
     1921 tgccgaaaca gccagtggat ctgcagcaat gaagaatgtc caggggagtg ccttgtcaca
     1981 ggtcaatcac acttcaagag ctttgacaac agatacttca ccttcagtgg gatctgccag
     2041 tacctgctgg cccgggattg ccaggaccac tccttctcca ttgtcattga gactgtccag
     2101 tgtgctgatg accgcgacgc tgtgtgcacc cgctccgtca ccgtccggct gcctggcctg
     2161 cacaacagcc ttgtgaaact gaagcatggg gcaggagttg ccatggatgg ccaggacgtc
     2221 cagctccccc tcctgaaagg tgacctccgc atccagcata cagtgacggc ctccgtgcgc
     2281 ctcagctacg gggaggacct gcagatggac tgggatggcc gcgggaggct gctggtgaag
     2341 ctgtcccccg tctatgccgg gaagacctgc ggcctgtgtg ggaattacaa tggcaaccag
     2401 ggcgacgact tccttacccc ctctgggctg gcggagcccc gggtggagga cttcgggaac
     2461 gcctggaagc tgcacgggga ctgccaggac ctgcagaagc agcacagcga tccctgcgcc
     2521 ctcaacccgc gcatgaccag gttctccgag gaggcgtgcg cggtcctgac gtcccccaca
     2581 ttcgaggcct gccatcgtgc cgtcagcccg ctgccctacc tgcggaactg ccgctacgac
     2641 gtgtgctcct gctcggacgg ccgcgagtgc ctgtgcggcg ccctggccag ctatgccgcg
     2701 gcctgcgcgg ggagaggcgt gcgcgtcgcg tggcgcgagc caggccgctg tgagctgaac
     2761 tgcccgaaag gccaggtgta cctgcagtgc gggaccccct gcaacctgac ctgccgctct
     2821 ctctcttacc cggatgagga atgcaatgag gcctgcctgg agggctgctt ctgcccccca
     2881 gggctctaca tggatgagag gggggactgc gtgcccaagg cccagtgccc ctgttactat
     2941 gacggtgaga tcttccagcc agaagacatc ttctcagacc atcacaccat gtgctactgt
     3001 gaggatggct tcatgcactg taccatgagt ggagtccccg gaagcttgct gcctgacgct
     3061 gtcctcagca gtcccctgtc tcatcgcagc aaaaggagcc tatcctgtcg gccccccatg
     3121 gtcaagctgg tgtgtcccgc tgacaacctg cgggctgaag ggctcgagtg taccaaaacg
     3181 tgccagaact atgacctgga gtgcatgagc atgggctgtg tctctggctg cctctgcccc
     3241 ccgggcatgg tccggcatga gaacagatgt gtggccctgg aaaggtgtcc ctgcttccat
     3301 cagggcaagg agtatgcccc tggagaaaca gtgaagattg gctgcaacac ttgtgtctgt
     3361 cgggaccgga agtggaactg cacagaccat gtgtgtgatg ccacgtgctc cacgatcggc
     3421 atggcccact acctcacctt cgacgggctc aaatacctgt tccccgggga gtgccagtac
     3481 gttctggtgc aggattactg cggcagtaac cctgggacct ttcggatcct agtggggaat
     3541 aagggatgca gccacccctc agtgaaatgc aagaaacggg tcaccatcct ggtggaggga
     3601 ggagagattg agctgtttga cggggaggtg aatgtgaaga ggcccatgaa ggatgagact
     3661 cactttgagg tggtggagtc tggccggtac atcattctgc tgctgggcaa agccctctcc
     3721 gtggtctggg accgccacct gagcatctcc gtggtcctga agcagacata ccaggagaaa
     3781 gtgtgtggcc tgtgtgggaa ttttgatggc atccagaaca atgacctcac cagcagcaac
     3841 ctccaagtgg aggaagaccc tgtggacttt gggaactcct ggaaagtgag ctcgcagtgt
     3901 gctgacacca gaaaagtgcc tctggactca tcccctgcca cctgccataa caacatcatg
     3961 aagcagacga tggtggattc ctcctgtaga atccttacca gtgacgtctt ccaggactgc
     4021 aacaagctgg tggaccccga gccatatctg gatgtctgca tttacgacac ctgctcctgt
     4081 gagtccattg gggactgcgc ctgcttctgc gacaccattg ctgcctatgc ccacgtgtgt
     4141 gcccagcatg gcaaggtggt gacctggagg acggccacat tgtgccccca gagctgcgag
     4201 gagaggaatc tccgggagaa cgggtatgag tgtgagtggc gctataacag ctgtgcacct
     4261 gcctgtcaag tcacgtgtca gcaccctgag ccactggcct gccctgtgca gtgtgtggag
     4321 ggctgccatg cccactgccc tccagggaaa atcctggatg agcttttgca gacctgcgtt
     4381 gaccctgaag actgtccagt gtgtgaggtg gctggccggc gttttgcctc aggaaagaaa
     4441 gtcaccttga atcccagtga ccctgagcac tgccagattt gccactgtga tgttgtcaac
     4501 ctcacctgtg aagcctgcca ggagccggga ggcctggtgg tgcctcccac agatgccccg
     4561 gtgagcccca ccactctgta tgtggaggac atcttcgaac ccccccacat ggcacaagtt
     4621 aacgtaggtc cggggctctt gggggtttcg accctggggc ccaagaggaa ctccatggtt
     4681 ctggatgtgg cgttcgtcct ggaaggatcg gacaaaattg gtgaagccga cttcaacagg
     4741 agcaaggagt tcatggagga ggtgattcag cggatggatg tgggccagga cagcatccac
     4801 gtcacggtgc tgcagtactc ctacatggtg accgtggagt accccttcag cgaggcacag
     4861 tccaaagggg acatcctgca gcgggtgcga gagatccgct accagggcgg caacaggacc
     4921 aacactgggc tggccctgcg gtacctctct gaccacagct tcttggtcag ccagggtgac
     4981 cgggagcagg cgcccaacct ggtctacatg gtcaccggaa atcctgcctc tgatgagatc
     5041 aagaggctgc ctggagacat ccaggtggtg cccattggag tgggccctaa tgccaacgtg
     5101 caggagctgg agaggattgg ctggcccaat gcccctatcc tcatccagga ctttgagacg
     5161 ctcccccgag aggctcctga cctggtgctg cagagttgtt gtaccggtga ggggctgcag
     5221 atccccaccc tctcccctgc acctgactgc agccagcccc tggacgtgat ccttctcctg
     5281 gatggctcct ccagtttccc agcttcttat tttgatgaaa tgaagagttt cgccaaggct
     5341 ttcatttcaa aagccaatat agggcctcgt ctcactcagg tgtcagtgct gcagtatgga
     5401 agcatcacca ccattgacgt gccatggaac gtggtcccgg agaaagccca tttgctgagc
     5461 cttgtggacg tcatgcagcg ggagggaggc cccagccaaa tcggggatgc cttgggcttt
     5521 gctgtgcgat acttgacttc agaaatgcat ggtgccaggc cgggagcctc aaaggcggtg
     5581 gtcatcctgg tcacggacgt ctctgtggat tcagtggatg cagcagctga tgccgccagg
     5641 tccaacagag tgacagtgtt ccctattgga attggagatc gctacgatgc agcccagcta
     5701 cggatcttgg caggcccagc aggcgactcc aacgtggtga agctccagcg aatcgaagac
     5761 ctccctacca tggtcacctt gggcaattcc ttcctccaca aactgtgctc tggatttgtt
     5821 aggatttgca tggatgagga tgggaatgag aagaggcccg gggacgtctg gaccttgcca
     5881 gaccagtgcc acaccgtgac ttgccagcca gatggccaga ccttgctgaa gagtcatcgg
     5941 gtcaactgtg accgggggct gaggccttcg tgccctaaca gccagtcccc tgttaaagtg
     6001 gaagagacct gtggctgccg ctggacctgc ccctgcgtgt gcacaggcag ctccactcgg
     6061 cacatcgtga cctttgatgg gcagaatttc aagctgactg gcagctgttc ttatgtccta
     6121 tttcaaaaca aggagcagga cctggaggtg attctccata atggtgcctg cagccctgga
     6181 gcaaggcagg gctgcatgaa atccatcgag gtgaagcaca gtgccctctc cgtcgagctg
     6241 cacagtgaca tggaggtgac ggtgaatggg agactggtct ctgttcctta cgtgggtggg
     6301 aacatggaag tcaacgttta tggtgccatc atgcatgagg tcagattcaa tcaccttggt
     6361 cacatcttca cattcactcc acaaaacaat gagttccaac tgcagctcag ccccaagact
     6421 tttgcttcaa agacgtatgg tctgtgtggg atctgtgatg agaacggagc caatgacttc
     6481 atgctgaggg atggcacagt caccacagac tggaaaacac ttgttcagga atggactgtg
     6541 cagcggccag ggcagacgtg ccagcccatc ctggaggagc agtgtcttgt ccccgacagc
     6601 tcccactgcc aggtcctcct cttaccactg tttgctgaat gccacaaggt cctggctcca
     6661 gccacattct atgccatctg ccagcaggac agttgccacc aggagcaagt gtgtgaggtg
     6721 atcgcctctt atgcccacct ctgtcggacc aacggggtct gcgttgactg gaggacacct
     6781 gatttctgtg ctatgtcatg cccaccatct ctggtctaca accactgtga gcatggctgt
     6841 ccccggcact gtgatggcaa cgtgagctcc tgtggggacc atccctccga aggctgtttc
     6901 tgccctccag ataaagtcat gttggaaggc agctgtgtcc ctgaagaggc ctgcactcag
     6961 tgcattggtg aggatggagt ccagcaccag ttcctggaag cctgggtccc ggaccaccag
     7021 ccctgtcaga tctgcacatg cctcagcggg cggaaggtca actgcacaac gcagccctgc
     7081 cccacggcca aagctcccac gtgtggcctg tgtgaagtag cccgcctccg ccagaatgca
     7141 gaccagtgct gccccgagta tgagtgtgtg tgtgacccag tgagctgtga cctgccccca
     7201 gtgcctcact gtgaacgtgg cctccagccc acactgacca accctggcga gtgcagaccc
     7261 aacttcacct gcgcctgcag gaaggaggag tgcaaaagag tgtccccacc ctcctgcccc
     7321 ccgcaccgtt tgcccaccct tcggaagacc cagtgctgtg atgagtatga gtgtgcctgc
     7381 aactgtgtca actccacagt gagctgtccc cttgggtact tggcctcaac cgccaccaat
     7441 gactgtggct gtaccacaac cacctgcctt cccgacaagg tgtgtgtcca ccgaagcacc
     7501 atctaccctg tgggccagtt ctgggaggag ggctgcgatg tgtgcacctg caccgacatg
     7561 gaggatgccg tgatgggcct ccgcgtggcc cagtgctccc agaagccctg tgaggacagc
     7621 tgtcggtcgg gcttcactta cgttctgcat gaaggcgagt gctgtggaag gtgcctgcca
     7681 tctgcctgtg aggtggtgac tggctcaccg cggggggact cccagtcttc ctggaagagt
     7741 gtcggctccc agtgggcctc cccggagaac ccctgcctca tcaatgagtg tgtccgagtg
     7801 aaggaggagg tctttataca acaaaggaac gtctcctgcc cccagctgga ggtccctgtc
     7861 tgcccctcgg gctttcagct gagctgtaag acctcagcgt gctgcccaag ctgtcgctgt
     7921 gagcgcatgg aggcctgcat gctcaatggc actgtcattg ggcccgggaa gactgtgatg
     7981 atcgatgtgt gcacgacctg ccgctgcatg gtgcaggtgg gggtcatctc tggattcaag
     8041 ctggagtgca ggaagaccac ctgcaacccc tgccccctgg gttacaagga agaaaataac
     8101 acaggtgaat gttgtgggag atgtttgcct acggcttgca ccattcagct aagaggagga
     8161 cagatcatga cactgaagcg tgatgagacg ctccaggatg gctgtgatac tcacttctgc
     8221 aaggtcaatg agagaggaga gtacttctgg gagaagaggg tcacaggctg cccacccttt
     8281 gatgaacaca agtgtctggc tgagggaggt aaaattatga aaattccagg cacctgctgt
     8341 gacacatgtg aggagcctga gtgcaacgac atcactgcca ggctgcagta tgtcaaggtg
     8401 ggaagctgta agtctgaagt agaggtggat atccactact gccagggcaa atgtgccagc
     8461 aaagccatgt actccattga catcaacgat gtgcaggacc agtgctcctg ctgctctccg
     8521 acacggacgg agcccatgca ggtggccctg cactgcacca atggctctgt tgtgtaccat
     8581 gaggttctca atgccatgga gtgcaaatgc tcccccagga agtgcagcaa gtgaggctgc
     8641 tgcagctgca tgggtgcctg ctgctgccgg aattctgcag atctagaggg cccgtttaaa
     8701 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     8761 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     8821 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     8881 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     8941 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgcactgta
     9001 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     9061 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     9121 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     9181 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     9241 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     9301 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     9361 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     9421 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     9481 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     9541 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     9601 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9661 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     9721 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     9781 atccattttc ggatctgatc agcacgtgat gaaaaagcct gaactcaccg cgacgtctgt
     9841 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     9901 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     9961 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
    10021 gctcccgatt ccggaagtgc ttgacattgg ggagttcagc gagagcctga cctattgcat
    10081 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
    10141 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
    10201 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
    10261 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
    10321 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
    10381 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
    10441 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
    10501 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
    10561 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
    10621 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
    10681 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
    10741 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
    10801 acgccccagc actcgtccga gggcaaagga atagcacgtg ctacgagatt tcgattccac
    10861 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
    10921 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
    10981 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    11041 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
    11101 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
    11161 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    11221 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    11281 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    11341 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    11401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    11461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    11521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    11581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    11641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    11701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    11761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    11821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    11881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    11941 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
    12001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    12061 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    12121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    12181 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    12241 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    12301 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    12361 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    12421 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    12481 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    12541 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    12601 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    12661 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    12721 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    12781 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    12841 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    12901 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    12961 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    13021 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    13081 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    13141 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    13201 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    13261 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
    13321 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
    13381 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
    13441 ggcttgaccg ac
//
