LOCUS       sequence-243922-        1365 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double mutant of wild type mmPylRS designed for incorporation of 
            non-canonical amino acids with mono-substituted phenylalanine 
            derivatives and tyrosinyl ethers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Wang YS, Fang X, Wallace AL, Wu B, Liu WR
  TITLE     A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad
            substrate spectrum.
  JOURNAL   J Am Chem Soc. 2012 Feb 15;134(6):2950-3. doi: 10.1021/ja211972x. 
            Epub 2012 Feb 6.
  PUBMED    22289053
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja211972x";
            journalName: "J Am Chem Soc"; date: "2012-02-15- 15"; volume: "134";
            issue: "6"; pages: "2950-3"
COMMENT     Sequence Label: pEVOL-pylT-N346A/C348A
FEATURES             Location/Qualifiers
     source          1..1365
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggataaaa aaccactaaa cactctgata tctgcaaccg ggctctggat gtccaggacc
       61 ggaacaattc ataaaataaa acaccacgaa gtctctcgaa gcaaaatcta tattgaaatg
      121 gcatgcggag accaccttgt tgtaaacaac tccaggagca gcaggactgc aagagcgctc
      181 aggcaccaca aatacaggaa gacctgcaaa cgctgcaggg tttcggatga ggatctcaat
      241 aagttcctca caaaggcaaa cgaagaccag acaagcgtaa aagtcaaggt cgtttctgcc
      301 cctaccagaa cgaaaaaggc aatgccaaaa tccgttgcga gagccccgaa acctcttgag
      361 aatacagaag cggcacaggc tcaaccttct ggatctaaat tttcacctgc gataccggtt
      421 tccacccaag agtcagtttc tgtcccggca tctgtttcaa catcaatatc aagcatttct
      481 acaggagcaa ctgcatccgc actggtaaaa gggaatacga accccattac atccatgtct
      541 gcccctgttc aggcaagtgc ccccgcactt acgaagagcc agactgacag gcttgaagtc
      601 ctgttaaacc caaaagatga gatttccctg aattccggca agcctttcag ggagcttgag
      661 tccgaattgc tctctcgcag aaaaaaagac ctgcagcaga tctacgcgga agaaagggag
      721 aattatctgg ggaaactcga gcgtgaaatt accaggttct ttgtggacag gggttttctg
      781 gaaataaaat ccccgatcct gatccctctt gagtatatcg aaaggatggg cattgataat
      841 gataccgaac tttcaaaaca gatcttcagg gttgacaaga acttctgcct gagacccatg
      901 cttgctccaa acctttacaa ctacctgcgc aagcttgaca gggccctgcc tgatccaata
      961 aaaatttttg aaataggccc atgctacaga aaagagtccg acggcaaaga acacctcgaa
     1021 gagtttacca tgctggcgtt cgcgcagatg ggatcgggat gcacacggga aaatcttgaa
     1081 agcataatta cggacttcct gaaccacctg ggaattgatt tcaagatcgt aggcgattcc
     1141 tgcatggtct atggggatac ccttgatgta atgcacggag acctggaact ttcctctgca
     1201 gtagtcggac ccataccgct tgaccgggaa tggggtattg ataaaccctg gataggggca
     1261 ggtttcgggc tcgaacgcct tctaaaggtt aaacacgact ttaaaaatat caagagagct
     1321 gcaaggtccg agtcttacta taacgggatt tctaccaacc tgtaa
//
