LOCUS       sequence-243969-        5861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses iCre under the CAG promoter in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa 
            R, Kobayakawa K, Saito T, Imai T
  TITLE     Activity-dependent local protection and lateral inhibition control 
            synaptic competition in developing mitral cells in mice.
  JOURNAL   Dev Cell. 2023 Jul 24;58(14):1221-1236.e7. doi: 
            10.1016/j.devcel.2023.05.004. Epub 2023 Jun 7.
  PUBMED    37290446
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2023.05.004"; journalName: "Dev Cell"; date: 
            "2023-07-24- 24"; volume: "58"; issue: "14"; pages: "1221-1236.e7"
COMMENT     Sequence Label: pCAG-iCre
FEATURES             Location/Qualifiers
     source          1..5861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          160..1176
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1100..1122
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1184..1203
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1246..1266
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2381..2400)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2446..2501
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2500..2519)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2862..2878)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2862..2878)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2875..2897)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2886..2902
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2910..2916,2917..2934,2935..2940))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2955..2976
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3035..3230
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3081..3216
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3236..3370
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3273..3292)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3327..3346
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3438..3455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3609..4197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3689..3708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4368..5159,5160..5228))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4991..5010
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5229..5333)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5364..5743
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(5745..5861,1..159)
                     /label=chicken beta-actin promoter
ORIGIN
        1 gggggcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
       61 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
      121 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
      181 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      241 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
      301 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
      361 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
      421 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
      481 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
      541 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
      601 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
      661 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
      721 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
      781 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
      841 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
      901 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
      961 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     1021 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     1081 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     1141 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     1201 gtgctgtctc atcattttgg caaagaattc gccaccatga tggtgcccaa gaagaagagg
     1261 aaagtctcca acctgctgac tgtgcaccaa aacctgcctg ccctccctgt ggatgccacc
     1321 tctgatgaag tcaggaagaa cctgatggac atgttcaggg acaggcaggc cttctctgaa
     1381 cacacctgga agatgctcct gtctgtgtgc agatcctggg ctgcctggtg caagctgaac
     1441 aacaggaaat ggttccctgc tgaacctgag gatgtgaggg actacctcct gtacctgcaa
     1501 gccagaggcc tggctgtgaa gaccatccaa cagcacctgg gccagctcaa catgctgcac
     1561 aggagatctg gcctgcctcg cccttctgac tccaatgctg tgtccctggt gatgaggaga
     1621 atcagaaagg agaatgtgga tgctggggag agagccaagc aggccctggc ctttgaacgc
     1681 actgactttg accaagtcag atccctgatg gagaactctg acagatgcca ggacatcagg
     1741 aacctggcct tcctgggcat tgcctacaac accctgctgc gcattgccga aattgccaga
     1801 atcagagtga aggacatctc ccgcaccgat ggtgggagaa tgctgatcca cattggcagg
     1861 accaagaccc tggtgtccac agctggtgtg gagaaggccc tgtccctggg ggttaccaag
     1921 ctggtggaga gatggatctc tgtgtctggt gtggctgatg accccaacaa ctacctgttc
     1981 tgccgggtca gaaagaatgg tgtggctgcc ccttctgcca cctcccaact gtccacccgg
     2041 gccctggaag ggatctttga ggccacccac cgcctgatct atggtgccaa ggatgactct
     2101 gggcagagat acctggcctg gtctggccac tctgccagag tgggtgctgc cagggacatg
     2161 gccagggctg gtgtgtccat ccctgaaatc atgcaggctg gtggctggac caatgtgaac
     2221 attgtgatga actacatcag aaacctggac tctgagactg gggccatggt gaggctgctc
     2281 gaggatgggg actgagcggc cgcactcctc aggtgcaggc tgcctatcag aaggtggtgg
     2341 ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa
     2401 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     2461 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     2521 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     2581 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     2641 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     2701 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     2761 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     2821 ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     2881 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     2941 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     3001 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag
     3061 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3121 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3181 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
     3241 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3301 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     3361 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     3421 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3481 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3541 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3601 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3661 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3721 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3781 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3841 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3901 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3961 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4021 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4081 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4141 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4201 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4261 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4321 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4381 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4441 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4501 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4561 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4621 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4681 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4741 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4801 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4861 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4921 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4981 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5041 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5101 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5161 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5221 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5281 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5341 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat
     5401 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     5461 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     5521 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     5581 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     5641 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     5701 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
     5761 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
     5821 tttttaatta ttttgtgcag cgatgggggc gggggggggg g
//
