LOCUS       sequence-244183-       14607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14607)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 14607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pBPGAL4.1Uw
FEATURES             Location/Qualifiers
     source          1..14607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..207
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(232..237,238..255,256..262)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             316..975
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(382..401)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1317..1622
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1501..1520
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1663..1787)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2052..4697
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             4961..7606
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     complement(8102..8118)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8102..8118)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(9129..9743,9814..9945,10149..10464,
                     10526..11180,11255..11528,11930..12001))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     12453..12471
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    12696..12765
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(12852..13440)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12932..12951)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13611..14402,14403..14471))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14234..14253
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14472..14576)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      121 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      181 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      241 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      301 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      361 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      421 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      481 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      541 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      601 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      661 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      721 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      781 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      841 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      901 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      961 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     1021 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     1081 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     1141 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     1201 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     1261 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     1321 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1381 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1441 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1501 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1561 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1621 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     1681 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1741 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aaacggccgg
     1801 ccgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg ctgagagcat
     1861 cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta atatcctttg
     1921 aataagccaa ctttgaatca caagacgcat accaaacggt accagatccg ggatcccgta
     1981 agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagcccaagc ttgaagcaag
     2041 cctcaatcaa aatgaagctg ctgagtagta ttgaacaagc gtgtgacata tgccgactta
     2101 agaagctgaa gtgcagcaag gaaaagccga agtgcgccaa atgtcttaag aacaattggg
     2161 agtgccggta ctcgccaaag acgaagcgaa gccctttgac acgcgctcat ttgactgaag
     2221 tggagagtcg cctggagcgc cttgagcagt tgttcctcct catttttccc cgtgaagacc
     2281 tcgacatgat actgaagatg gatagtctcc aagatattaa ggccctgctc actggcctgt
     2341 ttgttcagga taacgtcaac aaggatgccg tgacggaccg ccttgcctcg gtggagacag
     2401 atatgccact taccttgcgg cagcatcgca tctcggctac cagcagtagt gaagaaagtt
     2461 ccaataaggg tcaacggcaa ctgacggtat cgatcgacag tgcggcccac cacgacaaca
     2521 gcaccattcc actggacttc atgcctcgtg acgcactgca tggatttgac tggagcgagg
     2581 aggatgatat gagcgacgga ctcccattcc tgaagaccga ccccaataac aatggtttct
     2641 ttggcgacgg cagcctgctg tgtatcctgc gaagtattgg ttttaagccg gaaaattaca
     2701 cgaacagtaa tgtcaatcgt cttcccacaa tgatcaccga ccgttacacg ctggccagtc
     2761 gcagtactac aagccgcctg ctgcaaagct atcttaacaa cttccacccc tactgcccca
     2821 tagtgcatag tcctactctc atgatgcttt acaacaatca gattgagata gcatcgaagg
     2881 accagtggca gatcctcttc aactgcattc tcgccatcgg cgcttggtgt attgaaggcg
     2941 agagcacaga tatcgacgtg ttttactatc aaaacgccaa gtcgcacctc acctcgaagg
     3001 tcttcgaaag tggaagcatt atcctcgtga cagccctcca tctgctgagt cgttataccc
     3061 agtggcgtca gaaaactaat acatcctaca actttcattc gttcagcatt cggatggcga
     3121 ttagcctggg acttaatcgt gacctcccaa gtagcttcag cgattcgtcg atcctggaac
     3181 agcgccggag gatttggtgg agtgtgtaca gctgggagat ccagctgtcc ctcttgtacg
     3241 gacgcagcat tcagctgagt cagaacacga tttccttccc gtccagcgtg gacgacgttc
     3301 aacggacaac tacgggcccg accatctacc acggtatcat tgagacggcc cgcttgcttc
     3361 aagtgtttac gaagatttat gagctggata agacagtgac cgcggagaag agtccgatct
     3421 gcgccaagaa atgcctgatg atctgcaatg agatagagga ggtgagccgc caggcaccca
     3481 agttcctgca gatggacata agcactacgg cgctgactaa tctcctcaaa gaacacccct
     3541 ggctgagttt cacccgcttc gagctgaagt ggaaacagct gagtctgatt atatacgttt
     3601 tgcgggactt tttcacaaat tttacgcaga agaagtcgca gcttgagcaa gatcagaacg
     3661 accatcagtc ctacgaggta aagaggtgca gcataatgct ctccgacgca gcccagcgca
     3721 ccgtgatgtc ggtgtcgtcc tatatggaca accataatgt gaccccttat ttcgcctgga
     3781 attgctccta ctatttgttc aacgccgtgt tggtcccaat caagactctt ctgagcaact
     3841 ccaaatcgaa cgccgagaat aacgagacag cacagctctt gcagcaaatt aacaccgtgt
     3901 tgatgctgtt gaagaaactc gctacattca agattcagac ttgcgaaaag tacatccagg
     3961 tgttggaaga ggtgtgcgcc ccattccttc tttcgcaatg cgcaattccc ctcccgcaca
     4021 taagttacaa taattccaac ggctccgcta tcaagaatat cgtcggctcc gctacaatcg
     4081 cgcagtatcc cacgctgcct gaggaaaatg ttaacaacat atcggtgaag tatgtaagtc
     4141 caggcagcgt cggcccgagc cctgtgccgc tgaaaagcgg agcctccttc agcgatctgg
     4201 tcaagctgct cagcaaccgc ccaccatccc gcaactcccc tgtcactata ccccgatcga
     4261 ccccgtcgca tcgtagcgtt accccattcc tgggacaaca gcagcaactg cagagtctgg
     4321 tgccacttac gccctcggca ttgtttggcg gagccaactt caaccagagt ggtaacattg
     4381 cggacagctc cttgagcttc acgtttacca acagctccaa cggtccaaac ttgatcacga
     4441 ctcagacaaa ctcccaggca ttgtcccagc ccattgccag ctcgaatgtg catgataact
     4501 ttatgaataa tgaaattact gcctccaaga ttgacgacgg caacaacagc aagccccttt
     4561 ccccaggctg gactgaccag acggcctaca atgctttcgg tatcaccact ggcatgttca
     4621 acaccacaac gatggacgac gtatataact atctttttga tgatgaggat actccaccga
     4681 acccaaagaa ggagtagaat gaatcgtaga tactgaaaaa ccccgcaagt tcacttcaac
     4741 tgtgcatcgt gcaccatctc aatttctttc atttatacat cgttttgcct tcttttatgt
     4801 aactatactc ctctaagttt caatcttggc catgtaacct ctgatctata gaatttttta
     4861 aatgactaga attaatgccc atcttttttt tggacctaaa ttcttcatga aaatatatta
     4921 cgagggctta ttcagaagct tgaagcaagc ctcaatcaaa atgaagctgc tgagtagtat
     4981 tgaacaagcg tgtgacatat gccgacttaa gaagctgaag tgcagcaagg aaaagccgaa
     5041 gtgcgccaaa tgtcttaaga acaattggga gtgccggtac tcgccaaaga cgaagcgaag
     5101 ccctttgaca cgcgctcatt tgactgaagt ggagagtcgc ctggagcgcc ttgagcagtt
     5161 gttcctcctc atttttcccc gtgaagacct cgacatgata ctgaagatgg atagtctcca
     5221 agatattaag gccctgctca ctggcctgtt tgttcaggat aacgtcaaca aggatgccgt
     5281 gacggaccgc cttgcctcgg tggagacaga tatgccactt accttgcggc agcatcgcat
     5341 ctcggctacc agcagtagtg aagaaagttc caataagggt caacggcaac tgacggtatc
     5401 gatcgacagt gcggcccacc acgacaacag caccattcca ctggacttca tgcctcgtga
     5461 cgcactgcat ggatttgact ggagcgagga ggatgatatg agcgacggac tcccattcct
     5521 gaagaccgac cccaataaca atggtttctt tggcgacggc agcctgctgt gtatcctgcg
     5581 aagtattggt tttaagccgg aaaattacac gaacagtaat gtcaatcgtc ttcccacaat
     5641 gatcaccgac cgttacacgc tggccagtcg cagtactaca agccgcctgc tgcaaagcta
     5701 tcttaacaac ttccacccct actgccccat agtgcatagt cctactctca tgatgcttta
     5761 caacaatcag attgagatag catcgaagga ccagtggcag atcctcttca actgcattct
     5821 cgccatcggc gcttggtgta ttgaaggcga gagcacagat atcgacgtgt tttactatca
     5881 aaacgccaag tcgcacctca cctcgaaggt cttcgaaagt ggaagcatta tcctcgtgac
     5941 agccctccat ctgctgagtc gttataccca gtggcgtcag aaaactaata catcctacaa
     6001 ctttcattcg ttcagcattc ggatggcgat tagcctggga cttaatcgtg acctcccaag
     6061 tagcttcagc gattcgtcga tcctggaaca gcgccggagg atttggtgga gtgtgtacag
     6121 ctgggagatc cagctgtccc tcttgtacgg acgcagcatt cagctgagtc agaacacgat
     6181 ttccttcccg tccagcgtgg acgacgttca acggacaact acgggcccga ccatctacca
     6241 cggtatcatt gagacggccc gcttgcttca agtgtttacg aagatttatg agctggataa
     6301 gacagtgacc gcggagaaga gtccgatctg cgccaagaaa tgcctgatga tctgcaatga
     6361 gatagaggag gtgagccgcc aggcacccaa gttcctgcag atggacataa gcactacggc
     6421 gctgactaat ctcctcaaag aacacccctg gctgagtttc acccgcttcg agctgaagtg
     6481 gaaacagctg agtctgatta tatacgtttt gcgggacttt ttcacaaatt ttacgcagaa
     6541 gaagtcgcag cttgagcaag atcagaacga ccatcagtcc tacgaggtaa agaggtgcag
     6601 cataatgctc tccgacgcag cccagcgcac cgtgatgtcg gtgtcgtcct atatggacaa
     6661 ccataatgtg accccttatt tcgcctggaa ttgctcctac tatttgttca acgccgtgtt
     6721 ggtcccaatc aagactcttc tgagcaactc caaatcgaac gccgagaata acgagacagc
     6781 acagctcttg cagcaaatta acaccgtgtt gatgctgttg aagaaactcg ctacattcaa
     6841 gattcagact tgcgaaaagt acatccaggt gttggaagag gtgtgcgccc cattccttct
     6901 ttcgcaatgc gcaattcccc tcccgcacat aagttacaat aattccaacg gctccgctat
     6961 caagaatatc gtcggctccg ctacaatcgc gcagtatccc acgctgcctg aggaaaatgt
     7021 taacaacata tcggtgaagt atgtaagtcc aggcagcgtc ggcccgagcc ctgtgccgct
     7081 gaaaagcgga gcctccttca gcgatctggt caagctgctc agcaaccgcc caccatcccg
     7141 caactcccct gtcactatac cccgatcgac cccgtcgcat cgtagcgtta ccccattcct
     7201 gggacaacag cagcaactgc agagtctggt gccacttacg ccctcggcat tgtttggcgg
     7261 agccaacttc aaccagagtg gtaacattgc ggacagctcc ttgagcttca cgtttaccaa
     7321 cagctccaac ggtccaaact tgatcacgac tcagacaaac tcccaggcat tgtcccagcc
     7381 cattgccagc tcgaatgtgc atgataactt tatgaataat gaaattactg cctccaagat
     7441 tgacgacggc aacaacagca agcccctttc cccaggctgg actgaccaga cggcctacaa
     7501 tgctttcggt atcaccactg gcatgttcaa caccacaacg atggacgacg tatataacta
     7561 tctttttgat gatgaggata ctccaccgaa cccaaagaag gagtagaatg aatcgtagat
     7621 actgaaaaac cccgcaagtt cacttcaact gtgcatcgtg caccatctca atttctttca
     7681 tttatacatc gttttgcctt cttttatgta actatactcc tctaagtttc aatcttggcc
     7741 atgtaacctc tgatctatag aattttttaa atgactagaa ttaatgccca tctttttttt
     7801 ggacctaaat tcttcatgaa aatatattac gagggcttat tcagaagctt atcgataccg
     7861 tcgactaaag ccaaatagaa aattattcag ttcctggctt aagtttttaa aagtgatatt
     7921 atttatttgg ttgtaaccaa ccaaaagaat gtaaataact aatacataat tatgttagtt
     7981 ttaagttagc aacaaattga ttttagctat attagctact tggttaataa atagaatata
     8041 tttatttaaa gataattgcg tttttattgt cagggagtga gtttgcttaa aaactcgttt
     8101 agatccacta gttctagagc ggccgctggc cacgggtgcg catgatcgtg ctcctgtcgt
     8161 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
     8221 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
     8281 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
     8341 ttatgttccg gaggcgcgcc ctagttccag tgaaatccaa gcattttcta aattaaatgt
     8401 attcttatta ttatagttgt tatttttgat atatataaac aacactatta tgcccaccat
     8461 ttttttgaga tgcatctaca caaggaacaa acactggatg tcactttcag ttcaaattgt
     8521 aacgctaatc actccgaaca ggtcacaaaa aattacctta aaaagtcata atattaaatt
     8581 agaataaata tagctgtgag ggaaatatat acaaatatat tggagcaaat aaattgtaca
     8641 tacaaatatt tattactaat ttctattgag acgaaatgaa ccactcggaa ccatttgagc
     8701 gaaccgaatc gcgcggaact aacgacagtc gctccaaggt cgtcgaacaa aaggtgaatg
     8761 tgttgcggag agcgggtggg agacagcgaa agagcaacta cgaaacgtgg tgtggtggag
     8821 gtgaattatg aagagggcgc gcgatttgaa aagtatgtat ataaaaaata tatcccggtg
     8881 ttttatgtag cgataaacga gtttttgatg taaggtatgc aggtgtgtaa gtcttttggt
     8941 tagaagacaa atccaaagtc tacttgtggg gatgttcgaa ggggaaatac ttgtattcta
     9001 taggtcatat cttgttttta ttggcacaaa tataattaca ttagcttttt gagggggcaa
     9061 taaacagtaa acacgatggt aataatggta aaaaaaaaaa acaagcagtt atttcggata
     9121 tatgtcggct actccttgcg tcgggcccga agtcttagag ccagatatgc gagcacccgg
     9181 aagctcacga tgagaatggc cagacccacg tagtccagcg gcagatcggc ggcggagaag
     9241 ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg tggtgttcga cgatgtgcag
     9301 ctaatttcgc ccggctccac gtccgcccat tggttaatca gcagaccctc gttggcgtaa
     9361 cggaaccatg agaggtacga caaccatttg aggtatactg gcaccgagcc cgagttcaag
     9421 aagaagccgc caaagagcag gaatggtatg ataaccggcg gacccacaga cagcgccatc
     9481 gaggtcgagg agctggcgca ggatattaga tatccgaagg acgttgacac attggccacc
     9541 agagtgacca gcgccaggca gttgaagaag tgcagcactc cggcccgcag tccgatcatc
     9601 ggataggcaa tcgccgtgaa gaccagtggc actgtgagaa aaagcggcaa ttcggcaatc
     9661 gttttgccca gaaagtatgt gtcacagcga taaagtcgac ttcgggcctc cctcataaaa
     9721 actggcagct ctgaggtgaa cacctaaatc gaatcgattc attagaaagt tagtaaatta
     9781 ttgaaatgca aatgtattct aaacatgact tacatttatc gtggcaaaga cgttttgaaa
     9841 ggtcatgttg gtcaggaaga ggaagatggc tccgttgata ttcatcacac ccacttgcgt
     9901 gagttgttgg cccaaaaaga tgaggccaat caagatggca accatctgca aattaaaatg
     9961 ttactcgcat ctcattaata ttcgcgagtt aaatgaaatt tatttatctt ctgcaaaact
    10021 ataaactata catctcattg aaaaaaacta agaagggtgt ggaatcaggc aattctatct
    10081 aaaatctagc gaatttgttt ccaagaattg taagcgttat atcatttgtt tccactggaa
    10141 ccactcaccg ttgtctgaat aagtcgcact tttacgagga gtggttcctt gagcaccgac
    10201 agccaggatc gccacaggac cgcccggaac tgcatgaacc aggtggcctt gtaggtgtac
    10261 ccattctccg gctgctccag tggcttctcc agatttttgg tggccaacaa ctgctccata
    10321 tcccgggcta ctttgctaat ggcaaaattg tcgcatatct tggcgatccg atcacgggac
    10381 tcgatctccc gtccgggcac aacggccaac acctgtacgt aaaagtccgc cggattgtag
    10441 ttggtaggac actgggcacc cacgctggat aggagttgag atgttatgta atactagata
    10501 cccttaataa acacatcgaa ctcactagga aaagaagtcg acggcttcgc tgggagtgcc
    10561 caagaaagct accctgccct cggccatcag aaggatcttg tcaaagagct caaacagctc
    10621 ggaagacggc tgatgaatgg tcaggatgac ggtcttgccc ttctgcgaca gcttcttcag
    10681 cacctggacg acgctgtggg cggtaaagga gtccagtccg gaggtgggct catcgcagat
    10741 cagaagcggc ggatcggtta gagcctcgga ggcgaatgcc agacgcttcc tttctccgcc
    10801 ggacagacct ttcaccctgc cgggcacacc gatgatcgtg tgctgacatt tgctgagcga
    10861 aagctcctgg atcacctgat ccacgcgggc cactcgctgc cgataggtca gatgtcgtgg
    10921 catccgcacc atggcttgga aaatcaggtg ttccctggcc gttagggagc cgataaagag
    10981 gtcatcctgc tggacatagg cgcacctggc ctgcatctcc ttggcgtcca caggttggcc
    11041 attgagcagt cgcatcccgg atggcgatac ttggatgccc tgcggcgatc gaaaggcaag
    11101 ggcattcagc agggtcgtct ttccggcacc ggaactgccc atcacggcca aaagttcgcc
    11161 cggataggcc acgccgcaaa ctgagtttca aattggtaat tggacccttt attaagattt
    11221 cacacagatc agccgactgc gaatagaaac tcaccgttct tgagcaaatg tttcctgggc
    11281 gccggtatgt gtcgctcgtt gcagaatagt ccgcgtgtcc ggttgaccag ctgccgccat
    11341 ccggagcccg gctgattgac cgccccaaag atgtccatat tgtgccaggc ataggtgagg
    11401 ttctcggcta gttggccgct ccctgaaccg gagtcctccg gcggactggg tggccggagc
    11461 gtgccgtagt ttttggcctg cccgaagccc tggttaatgc agctctgcga agccgctccg
    11521 ctgtcaccct gcaatgatag gggatctcaa atatcaacta caagcgttat gctcatctaa
    11581 ccccgaacaa aaagtacccc gaagtatcct acgaagtagg tttatacttt tatttatttt
    11641 ttgtgcatct aggatcagct taaaatatct ggttgttata ttttttgtaa aaaagaatat
    11701 agtcgaaaat gaatgccttt agatgtcttg atcatgatat gatctcaaaa attgtcttat
    11761 atagcgagaa cagctaccag aataatctgt ttcgtgtcac tatttgtttg tgcaattgcg
    11821 gtttgggatt tttgtgggtc gcagttctca cgccgcagac aatttgatgt tgcaatcgca
    11881 gttcctatag atcaagtgaa cttaagatgt atgcacatgt actactcaca ttgttcagat
    11941 gctcggcaga tgggtgtttg ctgcctccgc gaattaatag ctcctgatcc tcttggccca
    12001 ttgccgggat ttttcacact ttcccctgct tacccaccca aaaccaatca ccaccccaat
    12061 cactcaaaaa acaaacaaaa ataagaagcg agaggagttt tggcacagca ctttgtgttt
    12121 aattgatggc gtaaaccgct tggagcttcg tcacgaaacc gctgacaaaa tgcaactgaa
    12181 ggcggacatt gacgctacgt aacgctacaa acggtggcga aagagatagc ggacgcagcg
    12241 gcgaaagaga cggcgatatt tctgtggaca gagaaggagg caaacagcgc tgactttgag
    12301 tggaatgtca ttttgagtga gaggtaatcg aaagaacctg gtacatcaaa tacccttgga
    12361 tcgaagtaaa tttaaaactg atcagataag ttcaatgata tccagtgcag taaaaaaaaa
    12421 aaatgttttt tttatctact ttccgcaaaa atgggtttta ttaacttaca tacatactag
    12481 gcgcgcccat atgttcggct tgtcgacatg cccgccgtga ccgtcgagaa cccgctgacg
    12541 ctgccccgcg tatccgcacc cgccgacgcc gtcgcacgtc ccgtgctcac cgtgaccacc
    12601 gcgcccagcg gtttcgaggg cgagggcttc ccggtgcgcc gcgcgttcgc cgggatcaac
    12661 taccgccacc tcgacccgtt catcatgatg gaccagatgg gtgaggtgga gtacgcgccc
    12721 ggggagccca agggcacgcc ctggcacccg caccgcggct tcgagaccgt gacctacatc
    12781 gtcgacggta acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    12841 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    12901 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    12961 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    13021 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    13081 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    13141 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    13201 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    13261 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    13321 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    13381 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    13441 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    13501 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    13561 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    13621 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    13681 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    13741 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    13801 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    13861 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    13921 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    13981 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    14041 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    14101 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    14161 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    14221 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    14281 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    14341 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    14401 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    14461 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    14521 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    14581 tttccccgaa aagtgccacc tgacgtc
//
