LOCUS       sequence-244268-        2630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Slot B, empty (step 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Santos-Moreno J, Schaerli Y
  TITLE     A Framework for the Modular and Combinatorial Assembly of Synthetic 
            Gene Circuits.
  JOURNAL   ACS Synth Biol. 2019 Jul 19;8(7):1691-1697. doi: 
            10.1021/acssynbio.9b00174. Epub 2019 Jun 24.
  PUBMED    31185158
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00174"; journalName: "ACS Synth Biol"; date: 
            "2019-07-19- 19"; volume: "8"; issue: "7"; pages: "1691-1697"
COMMENT     Sequence Label: pTU-B-013
FEATURES             Location/Qualifiers
     source          1..2630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(557..575)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      571..618
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      627..713
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      805..832
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(961..1752)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDDGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1753..1844)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1892..2630)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 ttttctactg aaccgctcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
       61 gtcagcccca tacgatataa gttgtaattc tcatgttagt catgccccgc gcccaccgga
      121 aggagctgac tgggttgctc ctaggatgca gtgactcgca catttccccg aaaagtgcca
      181 cctgaattcg aaatccctga aactgagact gtagaaaata agcttgtcca gactattgga
      241 tccaagagat ttctacacga ttgagcactg tctcctgcag gctcggtacc aaattccaga
      301 aaagaggcct cccgaaaggg gggccttttt tcgttttggt cctaatagat aaaggatagg
      361 tctggtagtg ttgttcgttc tcgcaggtaa atcaataata ctcagcagtt ccgtagactt
      421 ttcagtggga cagggtagcg ataacagata gattgtaata agacacagta ggtgctcgta
      481 gttgcgtgaa gagaaccgct caggaaatcc agtcagaagt attggtaatc gttgaaaact
      541 cagtcgactg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      601 gtcttgaggg gttttttgcc aggcatcaaa taaaacgaaa ggctcagtcg gaagactggg
      661 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg
      721 gagcggattt gaacgttgtg aagcaacggc ccggagggtg gcgggcagga cgcccgccat
      781 aaactgccag gcatcaaact aagcagaagg ccatcctgac ggatggcctt tttgcgtttc
      841 agatctaccg gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc
      901 gacgaccggg tcatcgtggc cggatcttgc ggcccctcgg cttgaacgaa ttgttagaca
      961 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
     1021 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
     1081 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
     1141 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
     1201 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
     1261 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
     1321 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
     1381 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
     1441 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
     1501 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
     1561 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
     1621 tggcttcagg ccgtcatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
     1681 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
     1741 cgcttccctc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     1801 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagcta gctcactcgg
     1861 tcgctacgct ccgggcgtga gactgcggcg ggcgctgcgg acacatacaa agttacccac
     1921 agattccgtg gataagcagg ggactaacat gtgaggcaaa acagcagggc cgcgccggtg
     1981 gcgtttttcc ataggctccg ccctcctgcc agagttcaca taaacagacg cttttccggt
     2041 gcatctgtgg gagccgtgag gctcaaccat gaatctgaca gtacgggcga aacccgacag
     2101 gacttaaaga tccccaccgt ttccggcggg tcgctccctc ttgcgctctc ctgttccgac
     2161 cctgccgttt accggatacc tgttccgcct ttctccctta cgggaagtgt ggcgctttct
     2221 catagctcac acactggtat ctcggctcgg tgtaggtcgt ttgctccaag ctgggctgta
     2281 agcaagaact ccccgttcag cccgactgct gcgccttatc cggtaactgt tcacttgagt
     2341 ccaacccgga aaagcacggt aaaacgccac tggcagcagc cattggtaac tgggagttcg
     2401 cagaggattt gtttagctaa acacgcggtt gctcttgaag tgtgcgccaa agtccggcta
     2461 cactggaagg acagatttgg ttgctgtgct ctgcgaaagc cagttaccac ggttaagcag
     2521 ttccccaact gacttaacct tcgatcaaac cacctcccca ggtggttttt tcgtttacag
     2581 ggcaaaagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
//
