LOCUS       sequence-244321-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV-ABEmax(TadA E59A, TadA*D108F) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Rees HA, Wilson C, Doman JL, Liu DR
  TITLE     Analysis and minimization of cellular RNA editing by DNA adenine 
            base editors.
  JOURNAL   Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. 
            eCollection 2019 May.
  PUBMED    31086823
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2019-05-8"; volume: "5(5):eaax5717. doi"; pages: " 
            10.1126/sciadv.aax5717. eCollection 2019 May"
COMMENT     Sequence Label: pCMV-ABEmax(TadA E59A, TadA*D108F)
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        74..453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        454..657
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     608..628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     699..718
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        699..717
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             767..787
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1976..1999,2000..2002,2003..6076)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1976..6076
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6119..6139
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6148..6165
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6188..6205)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6194..6418
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6489..6505)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6489..6505)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6502..6524)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6513..6529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6537..6543,6544..6561,6562..6567))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6582..6603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6720..6737)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6891..7479)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6971..6990)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7650..8441,8442..8510))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8273..8292
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8511..8615)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8694..8713)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
       61 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
      661 ttagtgaacc gtcagatccg ctagagatcc gcggccgcta atacgactca ctatagggag
      721 agccgccacc atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg
      781 gaaagtctct gaagtcgagt ttagccacga gtattggatg aggcacgcac tgaccctggc
      841 aaagcgagca tgggatgaaa gagaagtccc cgtgggcgcc gtgctggtgc acaacaatag
      901 agtgatcgga gagggatgga acaggccaat cggccgccac gaccctaccg cacacgcagc
      961 gatcatggca ctgaggcagg gaggcctggt catgcagaat taccgcctga tcgatgccac
     1021 cctgtatgtg acactggagc catgcgtgat gtgcgcagga gcaatgatcc acagcaggat
     1081 cggaagagtg gtgttcggag cacgggacgc caagaccggc gcagcaggct ccctgatgga
     1141 tgtgctgcac caccccggca tgaaccaccg ggtggagatc acagagggaa tcctggcaga
     1201 cgagtgcgcc gccctgctga gcgatttctt tagaatgcgg agacaggaga tcaaggccca
     1261 gaagaaggca cagagctcca ccgactctgg aggatctagc ggaggatcct ctggaagcga
     1321 gacaccaggc acaagcgagt ccgccacacc agagagctcc ggcggctcct ccggaggatc
     1381 ctctgaggtg gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag
     1441 ggcacgcgat gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat
     1501 cggcgagggc tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat
     1561 ggccctgaga cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta
     1621 cgtgacattc gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg
     1681 cgtggtgttt ggcgtgaggt ttgcaaaaac cggcgccgca ggctccctga tggacgtgct
     1741 gcactacccc ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg
     1801 tgccgccctg ctgtgctatt tctttcggat gcctagacag gtgttcaatg ctcagaagaa
     1861 ggcccagagc tccaccgact ccggaggatc tagcggaggc tcctctggct ctgagacacc
     1921 tggcacaagc gagagcgcaa cacctgaaag cagcgggggc agcagcgggg ggtcagacaa
     1981 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga
     2041 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
     2101 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
     2161 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
     2221 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
     2281 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
     2341 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     2401 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     2461 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
     2521 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
     2581 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
     2641 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc
     2701 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
     2761 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     2821 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     2881 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     2941 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     3001 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat
     3061 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     3121 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     3181 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     3241 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     3301 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     3361 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     3421 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     3481 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
     3541 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     3601 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     3661 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
     3721 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
     3781 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
     3841 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
     3901 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
     3961 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
     4021 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
     4081 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
     4141 cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt
     4201 ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat
     4261 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
     4321 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
     4381 cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta
     4441 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc
     4501 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa
     4561 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
     4621 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
     4681 cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga
     4741 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
     4801 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
     4861 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
     4921 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
     4981 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
     5041 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
     5101 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
     5161 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
     5221 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
     5281 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa
     5341 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt
     5401 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
     5461 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
     5521 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
     5581 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg
     5641 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
     5701 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
     5761 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
     5821 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
     5881 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
     5941 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct
     6001 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
     6061 tcagctggga ggtgactctg gcggctcaaa aagaaccgcc gacggcagcg aattcgagcc
     6121 caagaagaag aggaaagtct aaccggtcat catcaccatc accattgagt ttaaacccgc
     6181 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     6241 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     6301 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     6361 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     6421 tctgaggcgg aaagaaccag ctggggctcg ataccgtcga cctctagcta gagcttggcg
     6481 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6541 atacgagccg gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca
     6601 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6661 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6721 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6781 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6841 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6901 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6961 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7021 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7081 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7141 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7201 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7261 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7321 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7381 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7441 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7501 acggggtctg acactcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7561 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7621 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7681 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7741 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7801 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7861 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7921 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7981 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8041 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8101 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8161 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8221 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8281 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8341 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8401 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8461 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8521 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8581 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8641 gacgtcgacg gatcgggaga tcgatctccc gatcccctag ggtcgactct cagtacaatc
     8701 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     8761 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga c
//
