LOCUS       sequence-244331-        2300 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein 
            kinase II alpha (T286D/T305A/T306A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Chang JY, Nakahata Y, Hayano Y, Yasuda R
  TITLE     Mechanisms of Ca(2+)/calmodulin-dependent kinase II activation in 
            single dendritic spines.
  JOURNAL   Nat Commun. 2019 Jun 25;10(1):2784. doi: 10.1038/s41467-019-10694-z.
  PUBMED    31239443
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-06-25"; volume: "10(1):2784. doi"; pages: " 
            10.1038/s41467-019-10694-z"
COMMENT     Sequence Label: pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      51..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(57..59,60..62,63..773)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(102..123)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(363..382)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     710..731
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2258..2277)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgctagc gccaccatgg
       61 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      121 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      181 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      241 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
      301 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      361 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      421 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      481 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
      541 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      601 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      661 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      721 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac
      781 tcagatctcg agctatggct accatcacct gcacccgatt cacggaagag taccagctct
      841 tcgaggaact gggaaaggga gccttctccg tggtgcgcag gtgtgtgaag gtgctggctg
      901 gccaggagta tgctgccaag attatcaaca ccaagaagct ctcagccaga gatcaccaga
      961 agttggaacg cgaggcccgc atctgccgct tgttgaagca ccccaatatc gtccgactcc
     1021 atgacagcat ctccgaggag gggcaccact accttatctt cgatctggtc actggtgggg
     1081 agctgttcga agacattgtg gcccgggagt attacagtga ggctgatgcc agccactgta
     1141 tccagcagat cctggaggct gtgctacact gtcaccagat gggggtggtg catcgcgacc
     1201 tgaagcctga gaatctgttg ctggcttcga agctcaaggg tgctgcggtg aagctggcag
     1261 actttggcct ggccatagag gttgagggag agcagcaggc atggtttggg ttcgcaggga
     1321 cacctggata cctctcccca gaagtgctgc ggaaggaccc atacgggaag cctgtggacc
     1381 tgtgggcctg tggcgtcatc ctgtatatct tgctggttgg gtatccccca ttctgggatg
     1441 aggaccagca ccgcctgtac cagcagatca aagctggtgc ctacgatttc ccatcaccag
     1501 aatgggacac cgtcaccccg gaagccaagg atctgatcaa taagatgctg accatcaacc
     1561 cgtccaaacg catcacggcc gctgaggctc tcaagcaccc ctggatctcg caccgctcca
     1621 ctgtggcctc ctgcatgcac agacaggagg acgtggactg cctgaagaag ttcaatgcca
     1681 ggaggaaact gaagggagcc atcctcgccg ctatgctggc caccaggaac ttctccggag
     1741 ggaagagtgg aggaaacaag aagaatgatg gcgtgaagga atcctctgag agcaccaaca
     1801 ccaccatcga ggatgaagac accaaagtgc gcaaacagga aattatcaaa gtgacagagc
     1861 agctgatcga agccataagc aatggagact ttgaatccta cacgaagatg tgcgaccctg
     1921 gaatgacagc ctttgaaccg gaggccctgg ggaacctggt cgagggcctg gactttcatc
     1981 gattctattt tgaaaacctg tggtcccgga acagcaagcc cgtgcacacc accatcctga
     2041 accctcacat ccacctgatg ggtgacgagt cagcctgcat cgcctacatc cgcatcactc
     2101 agtacctgga tgcgggtggc atcccccgca cggcccagtc agaggagacc cgtgtctggc
     2161 accgcaggga tggaaaatgg cagatcgtcc acttccacag atctggggcg ccctccgtcc
     2221 tgccccattg agcggccgcg actctagagt cgacggatct ttttccctct gccaaaaatt
     2281 atggggacat catgaagccc
//
