LOCUS       sequence-244545-        2496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Slot C, empty (step 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Santos-Moreno J, Schaerli Y
  TITLE     A Framework for the Modular and Combinatorial Assembly of Synthetic 
            Gene Circuits.
  JOURNAL   ACS Synth Biol. 2019 Jul 19;8(7):1691-1697. doi: 
            10.1021/acssynbio.9b00174. Epub 2019 Jun 24.
  PUBMED    31185158
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00174"; journalName: "ACS Synth Biol"; date: 
            "2019-07-19- 19"; volume: "8"; issue: "7"; pages: "1691-1697"
COMMENT     Sequence Label: pTU-C-013
FEATURES             Location/Qualifiers
     source          1..2496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(423..441)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      437..484
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      493..579
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      671..698
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(827..1618)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDDGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1619..1710)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1758..2496)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 ttttctactg aaccgctcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
       61 gtcagcccca tacgatataa gttgtaattc tcatgttagt catgccccgc gcccaccgga
      121 aggagctgac tgggttgctc ctaggatgca gtgactcgca catttccccg aaaagtgcca
      181 cctgaattca gaagtattgg taatcgttga aaactcagtc gacgcactta ctgaagacgt
      241 cctattacac tcgtcgttgg aaactgaaga tgcggccgcg gaaacacaga aaaaagcccg
      301 cacctgacag tgcgggcttt ttttttcgac caaaggtagc gaacgacgag tcactgttga
      361 ggataaatac tttctctact aggcgcctgt tacacaggtc ctcagcggcg cgcctgctgc
      421 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      481 tttgccaggc atcaaataaa acgaaaggct cagtcggaag actgggcctt tcgttttatc
      541 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
      601 gttgtgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
      661 caaactaagc agaaggccat cctgacggat ggcctttttg cgtttcagat ctaccggtaa
      721 accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcat
      781 cgtggccgga tcttgcggcc cctcggcttg aacgaattgt tagacattat ttgccgacta
      841 ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg
      901 ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat
      961 actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc
     1021 cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag
     1081 cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct
     1141 gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt
     1201 ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac
     1261 ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac
     1321 gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc
     1381 tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt
     1441 catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgt
     1501 catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga
     1561 tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatac
     1621 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     1681 tatttgaatg tatttagaaa aataaacaaa tagctagctc actcggtcgc tacgctccgg
     1741 gcgtgagact gcggcgggcg ctgcggacac atacaaagtt acccacagat tccgtggata
     1801 agcaggggac taacatgtga ggcaaaacag cagggccgcg ccggtggcgt ttttccatag
     1861 gctccgccct cctgccagag ttcacataaa cagacgcttt tccggtgcat ctgtgggagc
     1921 cgtgaggctc aaccatgaat ctgacagtac gggcgaaacc cgacaggact taaagatccc
     1981 caccgtttcc ggcgggtcgc tccctcttgc gctctcctgt tccgaccctg ccgtttaccg
     2041 gatacctgtt ccgcctttct cccttacggg aagtgtggcg ctttctcata gctcacacac
     2101 tggtatctcg gctcggtgta ggtcgtttgc tccaagctgg gctgtaagca agaactcccc
     2161 gttcagcccg actgctgcgc cttatccggt aactgttcac ttgagtccaa cccggaaaag
     2221 cacggtaaaa cgccactggc agcagccatt ggtaactggg agttcgcaga ggatttgttt
     2281 agctaaacac gcggttgctc ttgaagtgtg cgccaaagtc cggctacact ggaaggacag
     2341 atttggttgc tgtgctctgc gaaagccagt taccacggtt aagcagttcc ccaactgact
     2401 taaccttcga tcaaaccacc tccccaggtg gttttttcgt ttacagggca aaagattacg
     2461 cgcagaaaaa aaggatctca agaagatcct ttgatc
//
