LOCUS       sequence-244664-        7603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral empty backbone control vector with CMV promoter and 
            Blasticidin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Hwang Y, Kim LC, Song W, Edwards DN, Cook RS, Chen J
  TITLE     Disruption of the Scaffolding Function of mLST8 Selectively Inhibits
            mTORC2 Assembly and Function and Suppresses mTORC2-Dependent Tumor 
            Growth In Vivo.
  JOURNAL   Cancer Res. 2019 Jul 1;79(13):3178-3184. doi: 
            10.1158/0008-5472.CAN-18-3658. Epub 2019 May 13.
  PUBMED    31085701
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-18-3658"; journalName: "Cancer Res"; date: 
            "2019-07-1- 1"; volume: "79"; issue: "13"; pages: "3178-3184"
COMMENT     Sequence Label: pLentiCMV-Blast
FEATURES             Location/Qualifiers
     source          1..7603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..642
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(107..127)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(525..536)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(693..713)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        698..1027
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      878..1013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        1075..1122
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1141..1539
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1626..1859
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1931..2052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1968..1987)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2022..2041
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2092..2227
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2247..2266)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2248..2266)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2276..2293)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2276..2292)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2285..2307)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2434..2889
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2521..2540)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2731..2752
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2915..3019
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3020..3088,3089..3880)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3238..3257)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4051..4639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4540..4559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4793..4810
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4927..4948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4963..4968,4969..4986,4987..4993)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5001..5017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5006..5028
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5025..5041
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5025..5041
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5060..5080
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5062..5080
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5108..5334
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5335..5515
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5562..5687
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6180..6413
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6598..6642
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6909..7025
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7048..7351
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7352..7555
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7506..7526
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7552..7576
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gacctccata gaagacaccg actctagagg atccaccggt cggccgtgtc gacaatcaac
       61 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
      121 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
      181 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
      241 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
      301 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
      361 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
      421 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
      481 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
      541 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
      601 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt ctgcagatat
      661 ccggttagta atgagtttgg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
      721 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
      781 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      841 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
      901 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
      961 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     1021 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     1081 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     1141 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     1201 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     1261 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     1321 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     1381 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     1441 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     1501 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacaattcga gctcggtacc
     1561 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     1621 gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg cttgtactgg
     1681 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     1741 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     1801 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     1861 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     1921 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     1981 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     2041 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     2101 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     2161 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     2221 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     2281 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     2341 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     2401 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     2461 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     2521 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     2581 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     2641 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     2701 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     2761 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     2821 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     2881 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2941 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3001 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3061 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3121 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3181 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     3241 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     3301 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     3361 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     3421 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     3481 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3541 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     3601 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     3661 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     3721 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     3781 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     3841 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     3901 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     3961 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4021 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4081 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4141 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4201 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4261 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4321 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4381 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4441 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4501 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4561 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4621 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4681 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4741 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     4801 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     4861 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     4921 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     4981 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     5041 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     5101 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     5161 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     5221 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     5281 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     5341 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5401 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5461 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     5521 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     5581 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     5641 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     5701 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     5761 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     5821 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     5881 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     5941 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     6001 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     6061 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     6121 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     6181 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     6241 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     6301 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     6361 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     6421 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     6481 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     6541 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     6601 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     6661 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     6721 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     6781 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     6841 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     6901 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     6961 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa
     7021 attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     7081 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     7141 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     7201 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     7261 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     7321 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     7381 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     7441 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     7501 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
     7561 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt ttt
//
