LOCUS       sequence-244676-        1351 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses siRNA resistant SENP2 (catalytic dead) in mammalian cells,
            Dox inducible in TetR cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Garvin AJ, Walker AK, Densham RM, Chauhan AS, Stone HR, Mackay HL, 
            Jamshad M, Starowicz K, Daza-Martin M, Ronson GE, Lanz AJ, Beesley 
            JF, Morris JR
  TITLE     The deSUMOylase SENP2 coordinates homologous recombination and 
            nonhomologous end joining by independent mechanisms.
  JOURNAL   Genes Dev. 2019 Mar 1;33(5-6):333-347. doi: 10.1101/gad.321125.118. 
            Epub 2019 Feb 22.
  PUBMED    30796017
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2019-03-1"; volume: "33(5-6):333-347. doi"; pages: " 
            10.1101/gad.321125.118. Epub 2019 Feb 22"
COMMENT     Sequence Label: FLAG-SENP2 C548A
FEATURES             Location/Qualifiers
     source          1..1351
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..53
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    55..73
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    76..94
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     104..128
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             228..251
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttnncgcaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta
       61 tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc
      121 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
      181 gatccagcct ccggactcta gcgtttaaac ttaagcttgg taccatggac tacaaggacg
      241 acgatgacaa gatcgattac agatggctgg ttaggattct cggcaccatt ttccgtttct
      301 gcgaccggtc ggtgccccct gcccgggccc tcctgaagag gcggcgctca gacagcactc
      361 tgttttctac agtggacact gatgaaatac cagccaaaag accaagatta gattgcttta
      421 ttcaccaagt gaaaaacagt ctctacaatg ctgccagctt atttggattc ccattccagc
      481 tgaccacaaa gcccatggta acttctgctt gtaatggaac acggaatgtg gccccttcag
      541 gagaggtatt ttcgaactct tcatcttgtg aactgacagg ttctggatcc tggaacaaca
      601 tgctgaaact gggtaataaa tctcctaatg gaataagtga ctatccaaag atcagagtga
      661 cagttacccg agatcagcca cgcagagtcc tgccttcctt tggttttact ttgaactcag
      721 aaggctgtaa tagaagacca ggtggccgtc gccatagcaa aggtaatcca gagagttctt
      781 taatgtggaa acctcaggaa caggctgtaa cagagatgat ttctgaagag agtggcaagg
      841 gtctgaggcg tccccattgt actgtggagg agggtgttca aaaagaggaa agagagaagt
      901 accgaaagtt attggaacga cttaaagaaa gtggtcatgg aaactctgtc tgtcctgtaa
      961 cttcaaatta tcacagttct caaagaagtc agatggacac attaaagacc aaaggctggg
     1021 gggaagagca aaatcacgga gtcaaaacaa ctcagtttgt tccaaaacaa tatagacttg
     1081 ttgaaacaag gggacctcta tgttcattga gaagtgaaaa gaggtgttca aaggggaaaa
     1141 ttactgatnn nnagacaatg gtcggaatca gatttgaaaa tgaaagtagg aggggaaacc
     1201 aactggagcc tgacctatca gagaaatgtc ggccnnannc ccntgggcnn ngaaacaatg
     1261 gcttacncgg aggaaagggn cataattgaa anaaggaang aattgnnnng gcaaagaagg
     1321 ganagaaaac ggacaattcc ttgaanttnn a
//
