LOCUS       sequence-244681-        6980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PSPC1 Y523F in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6980)
  AUTHORS   Lang YD, Chen HY, Ho CM, Shih JH, Hsu EC, Shen R, Lee YC, Chen JW, 
            Wu CY, Yeh HW, Chen RH, Jou YS
  TITLE     PSPC1-interchanged interactions with PTK6 and beta-catenin synergize
            oncogenic subcellular translocations and tumor progression.
  JOURNAL   Nat Commun. 2019 Dec 16;10(1):5716. doi: 10.1038/s41467-019-13665-6.
  PUBMED    31844057
REFERENCE   2  (bases 1 to 6980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-16"; volume: "10(1):5716. doi"; pages: " 
            10.1038/s41467-019-13665-6"
COMMENT     Sequence Label: pCDNA3-HA PSPC1 Y523F
FEATURES             Location/Qualifiers
     source          1..6980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        74..453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        454..657
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     608..628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     702..721
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        702..720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             746..772
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2136..2147
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        complement(2372..2390)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2373..2390)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2410..2427)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2416..2640
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2686..3114
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2773..2792)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2983..3004
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3123..3143)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3128..3457
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3308..3443
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3370..3389
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3524..4318
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3578..3597)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4188..4207
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4492..4613
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4529..4548)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4583..4602
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4662..4678)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4662..4678)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4675..4697)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4686..4702
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4710..4716,4717..4734,4735..4740))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4755..4776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4893..4910)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5064..5652)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5144..5163)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5823..6614,6615..6683))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6446..6465
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6684..6788)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6863..6882)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
       61 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
      661 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
      721 gagacccaag cttggtacca tggcttaccc ttatgatgtg ccagattatg cctcccggga
      781 tccaatgatg ttaagaggaa acctgaagca agtgcgcatt gagaaaaacc cggcccgcct
      841 tcgcgccctg gagtccgcgg tgggcgagag cgagccggcg gccgcggcag ccatggcgct
      901 cgctcttgcc ggggagccgg caccgcccgc gcccgcgcct ccagaggacc acccggacga
      961 ggagatgggg ttcactatcg acatcaagag tttcctcaag ccgggcgaga agacgtacac
     1021 gcagcgctgc cgcctcttcg tgggaaatct gcccaccgac atcacggagg aggacttcaa
     1081 gaggctcttc gaacgctatg gcgagcccag cgaagtcttc atcaaccggg accgtggctt
     1141 cggcttcatc cgcttggaat ccagaaccct ggctgaaatt gcaaaagcag agctggacgg
     1201 caccattctc aagagcagac ctctacggat tcgcttcgct acacatggag cagccttgac
     1261 tgtcaagaac ctttctccag ttgtttccaa tgagctgcta gagcaagcat tttctcagtt
     1321 tggtccagta gagaaagctg ttgtggttgt ggatgatcgc ggtagagcta caggaaaagg
     1381 ttttgtagag tttgcagcaa aacctcctgc acgaaaggct ctggaaagat gtggtgatgg
     1441 ggcattcttg ctaacaacga cccctcgtcc agtcattgtg gaacccatgg agcagtttga
     1501 tgatgaagat ggcttgccag agaagctgat gcagaaaact caacaatatc ataaggaaag
     1561 agaacaacca ccacgttttg ctcaacctgg gacatttgaa tttgagtatg catctcgatg
     1621 gaaggctctt gatgaaatgg aaaagcagca gcgtgagcag gttgatagaa acatcagaga
     1681 agccaaagag aaactggagg cagaaatgga agcagctagg catgaacacc aattaatgct
     1741 aatgaggcaa gatctaatga ggcgtcaaga agaactcaga cgcttggaag aactcagaaa
     1801 ccaagagttg caaaaacgga agcaaataca actaagacat gaagaggagc atcggcggcg
     1861 tgaggaagaa atgatccgac acagagaaca ggaggaactg aggcgacagc aagagggctt
     1921 taagccaaac tacatggaaa atagagaaca ggaaatgaga atgggtgata tgggtccccg
     1981 tggagcaata aacatgggag atgcgtttag cccagcccct gctggtaacc aaggtcctcc
     2041 tccaatgatg ggtatgaata tgaacaacag agcaactata cctggcccac caatgggtcc
     2101 tggtcctgcc atgggaccag aaggagccgc aaatatggga actccaatga tgccagataa
     2161 tggagcagtg cacaatgaca gatttcctca aggaccacca tctcagatgg gttcacctat
     2221 ggggagtaga acaggttctg aaacccctca agcaccaatg agtggtgtag gtcctgtgag
     2281 tggtggtcct ggtggctttg gtagaggaag tcaagggggc aactttgaag gccctaataa
     2341 gcgtcgtaga ttttaatcta gagggcccta ttctatagtg tcacctaaat gctagagctc
     2401 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     2461 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     2521 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     2581 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     2641 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     2701 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2761 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2821 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2881 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     2941 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3001 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     3061 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     3121 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3181 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     3241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3301 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3361 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     3421 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     3481 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     3541 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     3601 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     3661 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     3721 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     3781 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     3841 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3901 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     3961 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     4021 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     4081 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     4141 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     4201 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     4261 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     4321 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     4381 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     4441 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     4501 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4561 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     4621 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     4681 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4741 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4801 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     4861 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4921 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4981 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5041 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5101 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5161 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5221 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5281 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5341 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5401 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5461 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5521 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5581 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5641 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5701 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5761 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5821 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5881 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5941 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6001 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6061 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6121 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6181 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6241 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6301 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6361 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6421 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6481 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6541 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6601 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6661 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6721 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6781 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     6841 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     6901 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     6961 caaggcaagg cttgaccgac
//
