LOCUS       sequence-24471-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Lee HH, Kim YS, Kim KH, Heo I, Kim SK, Kim O, Kim HK, Yoon JY, Kim 
            HS, Kim do J, Lee SJ, Yoon HJ, Kim SJ, Lee BG, Song HK, Kim VN, Park
            CM, Suh SW
  TITLE     Structural and functional insights into dom34, a key component of 
            no-go mRNA decay.
  JOURNAL   Mol Cell. 2007 Sep 21. 27(6):938-50.
  PUBMED    17889667
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2007 Sep 21. 27(6):938-50."
COMMENT     Sequence Label: Ta1098(130-242)
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..106
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             457..474
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(527..545)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     primer_bind     complement(714..733)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgcganattc cctctgaata ttttgtttac tttaagaagg agatatacca tgggcagcag
       61 ccatcatcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata tggttacggt
      121 atacaccgct gtagccatgg atgaggatga agcgcagatt tttctgatac atccgtacgg
      181 aatccagcag gtcggcactg tctattcagg gagatccggt aaatacgcag agggcaacta
      241 ttccgaagcc tcatatttcg atcagattgt gaacgcgctg aagaactaca gcaacagtat
      301 cattattctc ggcccgggat ttgccaggga caggttcgca aggtactgcg ctcagagggg
      361 agtaaatgtc atcggcagtt ttccggcaaa caggactgat tctggcgctg tttatgaatt
      421 cataacctcg ccgatggggc gaagctgctg ctcgagcacc accaccacca ccactgagat
      481 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      541 ctagcataac cccttggggc ctctaaacgg gttgggaggg gtttttgngc tganaagnag
      601 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
      661 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      721 cgctttcttc ccttcnttct cgcacgttcg cngctttccn gtcagtctaa tcggggctcc
      781 nttagggtct gattatgctt acgcnctcga ccnaaaactg ataggtgagg tcnnatggcn
      841 tcgctgaaac gtttccntna gtgac
//
