LOCUS       sequence-244831-        2539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P7
FEATURES             Location/Qualifiers
     source          1..2539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(904..938)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1009..1668)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1583..1602
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1850..2438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2339..2358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgcaacgca tatgctgcaa tcacccaacg gaattaatta attaagtagt
       61 tacttacagt attgtaatgc catcccagcg tatcccagcc tagtgtatcc cagcctagcc
      121 tagcctaggc caaacctagg ccaaacctag gccaaaccta gccctctcta gcctagcgcc
      181 cagcagaaac accgatgaag caaagaagta acagcaggaa agaaaaacaa acacaacaaa
      241 aaaaaacaag cagcatagca tcaacagaaa tttctaaaga gaaccaaatt caccccagaa
      301 acaaccgcac aaatacgaca tccatccacc tttcttttat cttctttttc tgatctgata
      361 attagtttca tatacaatac gtagaaacag gcgcacagca cccagacctg gcttctgccc
      421 cagtgtataa gcaatgtagc ataattggaa aaaaaaacga aaaataccga aaataagtgg
      481 gaagctgggc cacaggagtg gggcgggatg cgactggttc tgagcgggac cgggtaataa
      541 ggttgaaaaa ctttgaattg atggaataag taacttcttt cttttcgctg gcgggagaag
      601 gaaaaaaaaa aatttttttt ccttctgttt agtactggga cattgagaag gcgtgtcaat
      661 tttgaataat tagagtggtc aaaaaaattt tttttgcttg ggataccctt tttcgataat
      721 gtaaattttt tttgaatata aaaggagatt gaaaaatttt ttctagcaga aatgttttca
      781 agttttaatt gcaagtttcg tttgagtatt cagttgtatt ttagttgatt tgtagtttat
      841 ttactagtat tctcatagtt ctaactccaa gagaagtaac attaaatatg tgagaccaga
      901 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
      961 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
     1021 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     1081 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     1141 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     1201 aaaactggtg aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc
     1261 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     1321 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     1381 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     1441 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     1501 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     1561 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     1621 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     1681 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     1741 gtgaaagttg gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt
     1801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2521 attctgtgga taaccgtag
//
