LOCUS       sequence-245293-       15100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the inducible expression of ER-targeted GFP1–10 
            fragment that can be used to detect a protein tagged with GFP11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15100)
  AUTHORS   Liu TY
  TITLE     Using Tripartite Split-sfGFP for the Study of Membrane 
            Protein-Protein Interactions.
  JOURNAL   Methods Mol Biol. 2021;2200:323-336. doi: 
            10.1007/978-1-0716-0880-7_15.
  PUBMED    33175385
REFERENCE   2  (bases 1 to 15100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2021"; volume: "2200:323-336. doi"; pages: " 
            10.1007/978-1-0716-0880-7_15"
COMMENT     Sequence Label: UBQ10:sXVE:ER-GFP1–10
FEATURES             Location/Qualifiers
     source          1..15100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(28..831)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(139..158)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     749..768
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(881..1060)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4322..4368
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      5202..5454
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5621..5645
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6110..6760
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(6948..6965)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7199..7218)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            7441..7550
                     /note="incP origin of transfer"
     CDS             7583..7954
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      8081..8792
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  9414..10181
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             10373..11167
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             11466..12614
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14210..14234
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(14481..14502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14693..14712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14837..14859
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14851..14868
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14852..14868
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(14881..15081)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
ORIGIN
        1 atccgtcgaa gctagcttgg gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
       61 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
      121 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
      181 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
      241 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
      301 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
      361 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
      421 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
      481 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
      541 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
      601 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc
      661 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
      721 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
      781 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc caagctccca tgggccctcg
      841 actagagtcg agatctggat tgagagtgaa tatgagactc taattggata ccgaggggaa
      901 tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt
      961 aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc
     1021 agaaacccgc ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa
     1081 cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg
     1141 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     1201 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
     1261 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
     1321 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
     1381 accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg
     1441 agtgcttgcg gcagcgtgaa gcttcgacga gtcagtaata aacggcgtca aagtggttgc
     1501 agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa
     1561 aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata cacgtgtcat
     1621 tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt
     1681 ttcttcttct tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt
     1741 tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat
     1801 ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt
     1861 atatgttctt tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat
     1921 cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa
     1981 taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta gttctggtgt
     2041 tagtttctag tttgtgcgat cgaatttgtc gattaatctg agtttttctg attaacagac
     2101 tagcccgcgg aaaatgaagg ctcttactgc tagacaacaa gaagtttttg atcttattag
     2161 agatcatatt tctcaaactg gaatgcctcc tactagagct gaaattgctc aaagacttgg
     2221 atttaggtct cctaatgctg ctgaagaaca tcttaaggct cttgctagaa agggagttat
     2281 tgaaattgtt tctggagctt caagaggaat tagacttctt caagaagaag aagaaggact
     2341 tcctcttgtt ggaagagttg ctgctggaga accttcttct gctcctccta ctgatgtttc
     2401 tcttggagat gaacttcatc ttgatggaga agatgttgct atggctcatg ctgatgctct
     2461 tgatgatttt gatcttgata tgcttggaga tggagattct cctggacctg gatttactcc
     2521 tcatgattct gctccttatg gagcacttga tatggctgat tttgaatttg aacaaatgtt
     2581 tactgatgct cttggaattg atgaatatgg aggagatcct tctgctggag atatgagagc
     2641 tgctaatctt tggccttctc ctcttatgat taagaggtct aagaagaact ctcttgctct
     2701 ttctcttact gctgaccaaa tggtttctgc tcttcttgat gctgaacctc ctattcttta
     2761 ttctgaatat gatcctacta gacctttttc tgaggcttct atgatgggac ttcttactaa
     2821 tcttgctgat agagaacttg ttcatatgat taattgggct aagagagttc ctggatttgt
     2881 tgatcttact cttcatgacc aagttcatct tcttgaatgt gcttggcttg aaattcttat
     2941 gattggactt gtttggaggt ctatggaaca tcctgttaaa cttctttttg ctcctaatct
     3001 tcttcttgat agaaatcaag gaaagtgtgt tgaaggaatg gttgaaattt ttgatatgct
     3061 tcttgctact tcttcaagat ttagaatgat gaatcttcaa ggagaagaat ttgtttgtct
     3121 taagtctatt attcttctta attctggagt ttatactttt ctttcttcta ctcttaagtc
     3181 tcttgaagaa aaggatcata ttcatagagt tcttgataag attactgata ctcttattca
     3241 tcttatggct aaggctggac ttactcttca acaacaacat caaagacttg ctcaacttct
     3301 tcttattctt tctcatatta gacatatgtc taataaggga atggaacatc tttattctat
     3361 gaagtgtaag aatgttgttc ctctttatga tcttcttctt gaaatgcttg atgctcatag
     3421 acttcatgct cctacttcaa gaggaggagc ttctgttgaa gaaactgacc aatctcatct
     3481 tgctactgct ggatctactt cttctcattc tcttcaaaag tattatatta ctggagaagc
     3541 tgaaggattt cctgctactg tttgaagatc ttgatcaagc tccctggcca attcccagag
     3601 atgttagctg aaatcatcac taatcagata ccaaaatatt caaatggaaa tatcaaatag
     3661 cttctgtttc atcaaaaatg actcgaccta actgagtaag catgcttgtt cgagtattat
     3721 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt
     3781 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga
     3841 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt
     3901 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa
     3961 tcgtggcctc taatgaccga agttaatatg aggagtaaaa catcccaaac gcggccgcaa
     4021 ccggtcttgc atccagctgg gctgcaggtc gaggctaaaa aactaatcgc attatcatcc
     4081 cctcgacgta ctgtacatat aaccactggt tttatataca gcagtactgt acatataacc
     4141 actggtttta tatacagcag tcgacgtact gtacatataa ccactggttt tatatacagc
     4201 agtactgtac atataaccac tggttttata tacagcagtc gaggtaagat tagatatgga
     4261 tatgtatatg gatatgtata tggtggtaat gccatgtaat atgctcgact ctaggatctt
     4321 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaagct
     4381 agtcgactct agccaaatct aggggatccg gcgcgcccgg gttaattaac tcgagatgaa
     4441 ggtacaggag ggtttgttcg tggtggctgt tttctacctt gcttatacgc agctagtcaa
     4501 ggggcaacct cgcaaggagt gcggatccat gtccaaagga gaagaacttt tcactggagt
     4561 tgtcccaatt cttgtcgaat tagatggtga tgttaatggg cacaaatttt cagtccgtgg
     4621 agagggtgaa ggtgatgcaa caatcggaaa acttacactt aaatttattt gcactactgg
     4681 aaaactacct gttccctggc caacacttgt cactaccctt acctatggtg ttcaatgctt
     4741 ttcaagatac ccagatcaca tgaagcggca cgacttcttc aagagcgcca tgcctgaggg
     4801 atacgtgcag gagaggacca tctcgttcaa ggacgacggg aaatacaaga cacgtgctgt
     4861 tgtcaagttt gagggagaca ccctcgtcaa caggatcgag cttaagggaa cagatttcaa
     4921 ggaggacgga aacatcctcg gccacaagtt ggaatacaac tttaactccc acaacgtata
     4981 catcacggcc gacaagcaaa agaacggcat caaagccaac ttcacagtac gccacaacgt
     5041 tgaagacggc tctgtgcaac tcgctgatca ttatcaacaa aatactccaa ttggcgatgg
     5101 ccctgtcctt ttaccagaca accattacct gtccacacaa acagtccttt ccaaagatcc
     5161 caacgaaaag catgacgagc tgtaagagct cgaatttccc cgatcgttca aacatttggc
     5221 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
     5281 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     5341 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     5401 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat
     5461 tcagatcggc tgagtggctc cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg
     5521 cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc ttaattctcc gctcatgatc
     5581 agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg
     5641 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
     5701 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccca gatctggcgc
     5761 cggccagcga gacgagcaag attggccgcc gcccgaaacg atccgacagc gcgcccagca
     5821 caggtgcgca ggcaaattgc accaacgcat acagcgccag cagaatgcca tagtgggcgg
     5881 tgacgtcgtt cgagtgaacc agatcgcgca ggaggcccgg cagcaccggc ataatcaggc
     5941 cgatgccgac agcgtcgagc gcgacagtgc tcagaattac gatcaggggt atgttgggtt
     6001 tcacgtctgg cctccggacc agcctccgct ggtccgattg aacgcgcgga ttctttatca
     6061 ctgataagtt ggtggacata ttatgtttat cagtgataaa gtgtcaagca tgacaaagtt
     6121 gcagccgaat acagtgatcc gtgccgccct ggacctgttg aacgaggtcg gcgtagacgg
     6181 tctgacgaca cgcaaactgg cggaacggtt gggggttcag cagccggcgc tttactggca
     6241 cttcaggaac aagcgggcgc tgctcgacgc actggccgaa gccatgctgg cggagaatca
     6301 tacgcattcg gtgccgagag ccgacgacga ctggcgctca tttctgatcg ggaatgcccg
     6361 cagcttcagg caggcgctgc tcgcctaccg cgatggcgcg cgcatccatg ccggcacgcg
     6421 accgggcgca ccgcagatgg aaacggccga cgcgcagctt cgcttcctct gcgaggcggg
     6481 tttttcggcc ggggacgccg tcaatgcgct gatgacaatc agctacttca ctgttggggc
     6541 cgtgcttgag gagcaggccg gcgacagcga tgccggcgag cgcggcggca ccgttgaaca
     6601 ggctccgctc tcgccgctgt tgcgggccgc gatagacgcc ttcgacgaag ccggtccgga
     6661 cgcagcgttc gagcagggac tcgcggtgat tgtcgatgga ttggcgaaaa ggaggctcgt
     6721 tgtcaggaac gttgaaggac cgagaaaggg tgacgattga tcaggaccgc tgccggagcg
     6781 caacccactc actacagcag agccatgtag acaacatccc ctcccccttt ccaccgcgtc
     6841 agacgcccgt agcagcccgc tacgggcttt ttcatgccct gccctagcgt ccaagcctca
     6901 cggccgcgct cggcctctct ggcggccttc tggcgctctt ccgcttcctc gctcactgac
     6961 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7021 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7081 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7141 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7201 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7261 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttt ccgctgcata
     7321 accctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata
     7381 tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc
     7441 gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta
     7501 ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccgg ctaccgccgg
     7561 cgtaacagat gagggcaagc ggatggctga tgaaaccaag ccaaccagga agggcagccc
     7621 acctatcaag gtgtactgcc ttccagacga acgaagagcg attgaggaaa aggcggcggc
     7681 ggccggcatg agcctgtcgg cctacctgct ggccgtcggc cagggctaca aaatcacggg
     7741 cgtcgtggac tatgagcacg tccgcgagct ggcccgcatc aatggcgacc tgggccgcct
     7801 gggcggcctg ctgaaactct ggctcaccga cgacccgcgc acggcgcggt tcggtgatgc
     7861 cacgatcctc gccctgctgg cgaagatcga agagaagcag gacgagcttg gcaaggtcat
     7921 gatgggcgtg gtccgcccga gggcagagcc atgacttttt tagccgctaa aacggccggg
     7981 gggtgcgcgt gattgccaag cacgtcccca tgcgctccat caagaagagc gacttcgcgg
     8041 agctggtgaa gtacatcacc gacgagcaag gcaagaccga gcgcctttcc gacgctcacc
     8101 gggctggttg ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa
     8161 acgccgtcga agccgtgtgc gagacaccgc ggccgccggc gttgtggata cctcgcggaa
     8221 aacttggccc tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc
     8281 ggcgcggcgt tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca
     8341 gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca
     8401 agcctgggga taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga
     8461 ggggcgcgat ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg
     8521 acatttgagg ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt
     8581 ttcggccacc gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt
     8641 tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct
     8701 tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc
     8761 ggccgcgaac ggcctcaccc caaaaatggc agcgctggca gtccttgcca ttgccgggat
     8821 cggggcagta acgggatggg cgatcagccc gagcgcgacg cccggaagca ttgacgtgcc
     8881 gcaggtgctg gcatcgacat tcagcgacca ggtgccgggc agtgagggcg gcggcctggg
     8941 tggcggcctg cccttcactt cggccgtcgg ggcattcacg gacttcatgg cggggccggc
     9001 aatttttacc ttgggcattc ttggcatagt ggtcgcgggt gccgtgctcg tgttcggggg
     9061 tgcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     9121 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     9181 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     9241 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     9301 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     9361 aatgcttgaa accaggacaa taaccttata gcttgtaaat tctatcataa ttgggtaatg
     9421 actccaactt attgatagtg ttttatgttc agataatgcc cgatgacttt gtcatgcagc
     9481 tccaccgatt ttgagaacga cagcgacttc cgtcccagcc gtgccaggtg ctgcctcaga
     9541 ttcaggttat gccgctcaat tcgctgcgta tatcgcttgc tgattacgtg cagctttccc
     9601 ttcaggcggg attcatacag cggccagcca tccgtcatcc atatcaccac gtcaaagggt
     9661 gacagcaggc tcataagacg ccccagcgtc gccatagtgc gttcaccgaa tacgtgcgca
     9721 acaaccgtct tccggagact gtcatacgcg taaaacagcc agcgctggcg cgatttagcc
     9781 ccgacatagc cccactgttc gtccatttcc gcgcagacga tgacgtcact gcccggctgt
     9841 atgcgcgagg ttaccgactg cggcctgagt tttttaagtg acgtaaaatc gtgttgaggc
     9901 caacgcccat aatgcgggct gttgcccggc atccaacgcc attcatggcc atatcaatga
     9961 ttttctggtg cgtaccgggt tgagaagcgg tgtaagtgaa ctgcagttgc catgttttac
    10021 ggcagtgaga gcagagatag cgctgatgtc cggcggtgct tttgccgtta cgcaccaccc
    10081 cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga gcacctcaaa
    10141 aacaccatca tacactaaat cagtaagttg gcagcatcac ccataattgt ggtttcaaaa
    10201 tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt
    10261 tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat
    10321 tagcttcttg gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa
    10381 aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac
    10441 ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt
    10501 aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat
    10561 gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga
    10621 tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga
    10681 agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca
    10741 ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt
    10801 ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac
    10861 tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga
    10921 acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt
    10981 aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt
    11041 ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga
    11101 cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt
    11161 gttttagtac ctagatgtgg cgcaacgatg ccggcgacaa gcaggagcgc accgacttct
    11221 tccgcatcaa gtgttttggc tctcaggccg aggcccacgg caagtatttg ggcaaggggt
    11281 cgctggtatt cgtgcagggc aagattcgga ataccaagta cgagaaggac ggccagacgg
    11341 tctacgggac cgacttcatt gccgataagg tggattatct ggacaccaag gcaccaggcg
    11401 ggtcaaatca ggaataaggg cacattgccc cggcgtgagt cggggcaatc ccgcaaggag
    11461 ggtgaatgaa tcggacgttt gaccggaagg catacaggca agaactgatc gacgcggggt
    11521 tttccgccga ggatgccgaa accatcgcaa gccgcaccgt catgcgtgcg ccccgcgaaa
    11581 ccttccagtc cgtcggctcg atggtccagc aagctacggc caagatcgag cgcgacagcg
    11641 tgcaactggc tccccctgcc ctgcccgcgc catcggccgc cgtggagcgt tcgcgtcgtc
    11701 tcgaacagga ggcggcaggt ttggcgaagt cgatgaccat cgacacgcga ggaactatga
    11761 cgaccaagaa gcgaaaaacc gccggcgagg acctggcaaa acaggtcagc gaggccaagc
    11821 aggccgcgtt gctgaaacac acgaagcagc agatcaagga aatgcagctt tccttgttcg
    11881 atattgcgcc gtggccggac acgatgcgag cgatgccaaa cgacacggcc cgctctgccc
    11941 tgttcaccac gcgcaacaag aaaatcccgc gcgaggcgct gcaaaacaag gtcattttcc
    12001 acgtcaacaa ggacgtgaag atcacctaca ccggcgtcga gctgcgggcc gacgatgacg
    12061 aactggtgtg gcagcaggtg ttggagtacg cgaagcgcac ccctatcggc gagccgatca
    12121 ccttcacgtt ctacgagctt tgccaggacc tgggctggtc gatcaatggc cggtattaca
    12181 cgaaggccga ggaatgcctg tcgcgcctac aggcgacggc gatgggcttc acgtccgacc
    12241 gcgttgggca cctggaatcg gtgtcgctgc tgcaccgctt ccgcgtcctg gaccgtggca
    12301 agaaaacgtc ccgttgccag gtcctgatcg acgaggaaat cgtcgtgctg tttgctggcg
    12361 accactacac gaaattcata tgggagaagt accgcaagct gtcgccgacg gcccgacgga
    12421 tgttcgacta tttcagctcg caccgggagc cgtacccgct caagctggaa accttccgcc
    12481 tcatgtgcgg atcggattcc acccgcgtga agaagtggcg cgagcaggtc ggcgaagcct
    12541 gcgaagagtt gcgaggcagc ggcctggtgg aacacgcctg ggtcaatgat gacctggtgc
    12601 attgcaaacg ctagggcctt gtggggtcag ttccggctgg gggttcagca gccagcgctt
    12661 tactggcatt tcaggaacaa gcgggcactg ctcgacgcac ttgcttcgct cagtatcgct
    12721 cgggacgcac ggcgcgctct acgaactgcc gataaacaga ggattaaaat tgacaattgt
    12781 gattaaggct cagattcgac ggcttggagc ggccgacgtg caggatttcc gcgagatccg
    12841 attgtcggcc ctgaagaaag ctccagagat gttcgggtcc gtttacgagc acgaggagaa
    12901 aaagcccatg gaggcgttcg ctgaacggtt gcgagatgcc gtggcattcg gcgcctacat
    12961 cgacggcgag atcattgggc tgtcggtctt caaacaggag gacggcccca aggacgctca
    13021 caaggcgcat ctgtccggcg ttttcgtgga gcccgaacag cgaggccgag gggtcgccgg
    13081 tatgctgctg cgggcgttgc cggcgggttt attgctcgtg atgatcgtcc gacagattcc
    13141 aacgggaatc tggtggatgc gcatcttcat cctcggcgca cttaatattt cgctattctg
    13201 gagcttgttg tttatttcgg tctaccgcct gccgggcggg gtcgcggcga cggtaggcgc
    13261 tgtgcagccg ctgatggtcg tgttcatctc tgccgctctg ctaggtagcc cgatacgatt
    13321 gatggcggtc ctgggggcta tttgcggaac tgcgggcgtg gcgctgttgg tgttgacacc
    13381 aaacgcagcg ctagatcctg tcggcgtcgc agcgggcctg gcgggggcgg tttccatggc
    13441 gttcggaacc gtgctgaccc gcaagtggca acctcccgtg cctctgctca cctttaccgc
    13501 ctggcaactg gcggccggag gacttctgct cgttccagta gctttagtgt ttgatccgcc
    13561 aatcccgatg cctacaggaa ccaatgttct cggcctggcg tggctcggcc tgatcggagc
    13621 gggtttaacc tacttccttt ggttccgggg gatctcgcga ctcgaaccta cagttgtttc
    13681 cttactgggc tttctcagcc ccagatctgg ggtcgatcag ccggggatgc atcaggccga
    13741 cagtcggaac ttcgggtccc cgacctgtac cattcggtga gcaatggata ggggagttga
    13801 tatcgtcaac gttcacttct aaagaaatag cgccactcag cttcctcagc ggctttatcc
    13861 agcgatttcc tattatgtcg gcatagttct caagatcgac agcctgtcac ggttaagcga
    13921 gaaatgaata agaaggctga taattcggat ctctgcgagg gagatgatat ttgatcacag
    13981 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
    14041 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
    14101 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
    14161 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
    14221 attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa
    14281 tgtactgggg tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc
    14341 gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa
    14401 tcctgtttga tggtggttcc gaaatcggca aaatccctta taaatcaaaa gaatagcccg
    14461 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
    14521 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
    14581 ccaaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
    14641 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
    14701 aagcgaaagg agcgggcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
    14761 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
    14821 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattccc
    14881 atcttgaaag aaatatagtt taaatattta ttgataaaat aacaagtcag gtattatagt
    14941 ccaagcaaaa acataaattt attgatgcaa gtttaaattc agaaatattt caataactga
    15001 ttatatcagc tggtacattg ccgtagatga aagactgagt gcgatattat gtgtaataca
    15061 taaattgatg atatagctag cttagctcat cgggggatcg
//
